Direct antagonistic activity of chitinase produced by Trichoderma sp. SANA20 as biological control agent for grey mould caused by Botrytis cinerea

AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

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The potential biocontrol agent Paenibacillus polymyxa TP3 produces fusaricidin-type compounds involved in the antagonism against gray mold pathogen Botrytis cinerea

Phytopathology ◽

10.1094/phyto-04-21-0178-r ◽

2021 ◽

Author(s):

Shuen-Huang Tsai ◽

Yu-Ting Chen ◽

Yu-Liang Yang ◽

Bo-Yi Lee ◽

Chien-Jui Huang ◽

...

Keyword(s):

Mass Spectrometry ◽

Botrytis Cinerea ◽

Biological Control Agent ◽

Imaging Mass Spectrometry ◽

Paenibacillus Polymyxa ◽

Field Trials ◽

Gray Mold ◽

Control Agent ◽

Antagonistic Activity ◽

New Variant

Paenibacillus polymyxa is a beneficial bacterium for plant health. Paenibacillus polymyxa TP3 exhibits antagonistic activity toward Botrytis cinerea and alleviates gray mold symptoms on the leaves of strawberry plants. Moreover, suppression of gray mold on the flowers and fruits of strawberry plants in field trials, including vegetative cells and endospores, was demonstrated, indicating the potential of strain TP3 as a biological control agent. To examine the anti-B. cinerea compounds produced by P. polymyxa TP3, matrix‐assisted laser‐desorption/ionization time‐of‐flight mass spectrometry was performed and fusaricidin-corresponding mass spectra were detected. Moreover, fusaricidin-related signals appeared in imaging mass spectrometry of TP3 when confronted with B. cinerea. By using liquid chromatography-mass spectrometry-based molecular networking approach, several fusaricidins were identified including a new variant of m/z 917.5455 with serine in the first position of the hexapeptide. Via advanced mass spectrometry and network analysis, fusaricidin-type compounds produced by P. polymyxa TP3 were efficiently disclosed and were presumed to play roles in the antagonism against gray mold pathogen B. cinerea.

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Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

The Scientific World JOURNAL ◽

10.1100/2012/723293 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

M. M. Rahman ◽

M. E. Ali ◽

A. A. Khan ◽

A. M. Akanda ◽

Md. Kamal Uddin ◽

...

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Erwinia Carotovora ◽

Soft Rot ◽

Control Agent ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Antagonistic Bacteria ◽

And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

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Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Toxins ◽

10.3390/toxins11060322 ◽

2019 ◽

Vol 11 (6) ◽

pp. 322 ◽

Cited By ~ 11

Author(s):

Shuwu Zhang ◽

Qi Zheng ◽

Bingliang Xu ◽

Jia Liu

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Fungal Pathogens ◽

Biological Control Agent ◽

Storage Period ◽

Control Agent ◽

Antagonistic Activity ◽

Postharvest Disease ◽

Economic Losses ◽

Control Mechanisms

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

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The potential of organic and inorganic soil amendments, and a biological control agent (Trichoderma sp.) for the management of Phytophthora root rot of papaw in far northern Queensland

Australasian Plant Pathology ◽

10.1071/ap02050 ◽

2002 ◽

Vol 31 (4) ◽

pp. 391 ◽

Cited By ~ 7

Author(s):

L. L. Vawdrey ◽

T. M. Martin ◽

J. De Faveri

Keyword(s):

Biological Control ◽

Root Rot ◽

Biological Control Agent ◽

Soil Amendments ◽

Control Agent ◽

Phytophthora Root Rot ◽

Trichoderma Sp

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Evaluating a preventive biological control agent applied on grapevines against Botrytis cinerea and its influence on winemaking

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.8977 ◽

2018 ◽

Vol 98 (12) ◽

pp. 4517-4526 ◽

Cited By ~ 6

Author(s):

Rocío Escribano-Viana ◽

Javier Portu ◽

Patrocinio Garijo ◽

Ana Rosa Gutiérrez ◽

Pilar Santamaría ◽

...

Keyword(s):

Biological Control ◽

Botrytis Cinerea ◽

Biological Control Agent ◽

Control Agent

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Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽

10.3390/agronomy9120891 ◽

2019 ◽

Vol 9 (12) ◽

pp. 891

Author(s):

Mila Santos ◽

Fernando Diánez ◽

Alejandro Moreno-Gavíra ◽

Brenda Sánchez-Montesinos ◽

Francisco J. Gea

Keyword(s):

Biological Control ◽

Fusarium Oxysporum ◽

Biocontrol Agent ◽

Biological Control Agent ◽

Control Agent ◽

Phytopathogenic Fungi ◽

Antagonistic Activity ◽

Dual Culture ◽

Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

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Biological Control of Fungal Diseases by Trichoderma aggressivum f. europaeum and Its Compatibility with Fungicides

Journal of Fungi ◽

10.3390/jof7080598 ◽

2021 ◽

Vol 7 (8) ◽

pp. 598

Author(s):

Brenda Sánchez-Montesinos ◽

Mila Santos ◽

Alejandro Moreno-Gavíra ◽

Teresa Marín-Rodulfo ◽

Francisco J. Gea ◽

...

Keyword(s):

Biological Control ◽

Growth Rates ◽

Biological Control Agent ◽

Disease Incidence ◽

Management Strategies ◽

High Growth ◽

Control Agent ◽

Antagonistic Activity ◽

La Mancha

Our purpose was to evaluate the ability of Trichoderma aggressivum f. europaeum as a biological control agent against diseases from fungal phytopathogens. Twelve isolates of T. aggressivum f. europaeum were obtained from several substrates used for Agaricus bisporus cultivation from farms in Castilla-La Mancha (Spain). Growth rates of the 12 isolates were determined, and their antagonistic activity was analysed in vitro against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium solani f. cucurbitae, Pythium aphanidermatum, Rhizoctonia solani, and Mycosphaerella melonis, and all isolates had high growth rates. T. aggressivum f. europaeum showed high antagonistic activity for different phytopathogens, greater than 80%, except for P. aphanidermatum at approximately 65%. The most effective isolate, T. aggressivum f. europaeum TAET1, inhibited B. cinerea, S. sclerotiorum, and M. melonis growth by 100% in detached leaves assay and inhibited germination of S. sclerotiorum sclerotia. Disease incidence and severity in plant assays for pathosystems ranged from 22% for F. solani to 80% for M. melonis. This isolate reduced the incidence of Podosphaera xanthii in zucchini leaves by 66.78%. The high compatibility by this isolate with fungicides could allow its use in combination with different pest management strategies. Based on the results, T. aggressivum f. europaeum TAET1 should be considered for studies in commercial greenhouses as a biological control agent.

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Draft Genome Sequence of Bacillus subtilis ALBA01, a Strain with Antagonistic Activity against the Soilborne Fungal Pathogen of Onion Setophoma terrestris

Genome Announcements ◽

10.1128/genomea.00455-16 ◽

2016 ◽

Vol 4 (3) ◽

Cited By ~ 4

Author(s):

Andrea G. Albarracín Orio ◽

Romina A. Tobares ◽

Daniel A. Ducasse ◽

Andrea M. Smania

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Activity ◽

Genome Sequence ◽

Fungal Pathogen ◽

Biological Control Agent ◽

Draft Genome ◽

Control Agent ◽

Antagonistic Activity ◽

Setophoma Terrestris

Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris .

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