scholarly journals Function of ex vivo stimulated Natural killer T cells from patients with chronic HIV-1 infection

2019 ◽  
Author(s):  
Prakash Thapa ◽  
Pramod Nehete ◽  
Hong He ◽  
Bharti Nehete ◽  
Stephanie J. Buchl ◽  
...  
Keyword(s):  
Natural Killer ◽  
Peripheral Blood Mononuclear Cells ◽  
Immune Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Nkt Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Hiv 1

AbstractNatural killer T (NKT) cells are innate immune cells that are responsible for the first line of antiviral defense, through crosstalk with downstream antigen-presenting cells, natural killer cells, and adaptive immune cells. Previous studies have indicated that NKT cell function is severely impaired in patients with chronic HIV-1 infection. It was reported that alpha-galactosylceramide, a potent agonist antigen for NKT cells, failed to trigger the expansion of NKT cells, or the production of anti-viral cytokines by NKT cells from HIV-1 infected patients in an in vitro assay, in which peripheral blood mononuclear cells (PBMCs) were cultured in the presence of alpha-galactosylceramide. In this study, we stimulated banked peripheral blood mononuclear cells from HIV-1-infected patients with dendritic cells (DC) generated ex vivo and loaded with alpha-galactosylceramide. The results showed that NKT cells were expanded in HIV infected subjects except in patients with advanced AIDS. Expanded NKT cells were capable of producing antiviral cytokines. Our results indicate that NKT cells in HIV infected individuals are potential targets for therapeutic intervention.

scholarly journals Profil Peripheral Blood Mononuclear Cells (PBMC) Pasien dengan Berbagai Usia Menggunakan Flow Cytometry di Klinik Hayandra

2018 ◽  
Vol 4 (4) ◽  
pp. 208
Author(s):  
Masayu Nadhira ◽  
Riris Lindiawati Puspitasari ◽  
Karina F Moegni ◽  
Imam Rosadi ◽  
Iis Rosliana
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Peripheral Blood Mononuclear Cells ◽  
Immune Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Group 2 ◽  
Cluster Of Differentiation

<p><em>Abstrak</em> - <strong>Profil Peripheral Blood Mononuclear Cells (PBMC) setiap orang dapat berbeda-beda. Salah satu faktor yang diduga mempengaruhi adanya perbedaan tersebut adalah usia. Manusia berusia lanjut lebih mudah terserang penyakit karena terjadinya penurunan fungsi dan jumlah sel imun. Oleh karena itu, perlu dilakukan analisis komposisi PBMC untuk mengetahui profil PBMC seseorang berdasarkan usia, sehingga dapat diketahui jumlah sel imun dalam tubuh manusia. Tujuan dari penelitian ini adalah untuk menganalisis profil PBMC pasien dengan berbagai usia di Klinik Hayandra menggunakan flow cytometry. Manfaat dilakukannya kegiatan penelitian ini adalah diperolehnya informasi mengenai profil PBMC pasien di Klinik Hayandra dan kaitannya dengan usia. Metode yang dilakukan diantaranya adalah isolasi PBMC, proses pewarnaan PBMC, dan penentuan populasi sel menggunakan flow cytometry. Cluster of Differentiation (CD) yang digunakan pada kegiatan penelitian ini diantaranya adalah CD3, CD19, CD8, CD56, dan CD314 atau disebut juga NKG2D (Natural Killer Group 2 member D). Hasil yang diperoleh pada kegiatan penelitian ini adalah ditemukannya korelasi negatif yang bermakna antara persentase jumlah sel limfosit T sitotoksik (CD3+/CD8+) dengan usia dan ditemukannya korelasi positif yang bermakna antara persentase jumlah sel NKT (CD3+/CD56+) dengan usia. Dengan demikian usia pasien dapat mempengaruhi jumlah sel imun pasien.</strong></p><p><em>Abstract</em> - <strong>Peripheral Blood Mononuclear Cells (PBMC) profiles of each person can different. One of the factors that allegedly affects the difference is age. Elderly human are more susceptible attacked disease due to decreased function and number of immune cells. Therefore, it is necessary to do analyze PBMC composition to know the PBMC profile of person, so that can be known the number of immune cells in the human body. The purpose of this research is to analyze the PBMC profile of patients with various ages in the Hayandra Clinic using flow cytometry. The benefit of doing this practical work is obtained information about the PBMC profile of patients in the Hayandra Clinic and its relation to age. Methods which are conducted among others are PBMC isolation, PBMC staining process, running flow cytometry, and determination of cell population. Cluster of Differentiation (CD) used in this practical work among others are CD3, CD19, CD8, CD56, and CD314 or also called NKG2D (Natural Killer Group 2 member D). The results obtained in this research were the found of significant negative correlation between percentage of cytotoxic T cell lymphocytes numbers (CD3+/CD8+) with age and found a significant positive correlation between percentage of NKT cell numbers (CD3+/CD56+) with age. So it can be concluded that the patient's age can affect the number of the patient immune cells.</strong></p><p><em><strong>Keywords</strong></em> - <em>Peripheral Blood Mononuclear Cells (PBMC), flow cytometry, age, Cluster of Differentiation (CD)</em></p>

Evidence for late stage compartmentalization of HIV-1 resistance mutations between lymph node and peripheral blood mononuclear cells

AIDS ◽  
2000 ◽  
Vol 14 (15) ◽  
pp. 2273-2281 ◽  
Author(s):  
Da' N. Haddad ◽  
Christopher Birch ◽  
Tracy Middleton ◽  
Dominic E. Dwyer ◽  
Anthony L. Cunningham ◽  
...  
Keyword(s):  
Lymph Node ◽  
Peripheral Blood Mononuclear Cells ◽  
Late Stage ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Resistance Mutations ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Hiv 1
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