scholarly journals A YAC-based contig of 1.5 Mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines.

1995 ◽  
Vol 5 (3) ◽  
pp. 233-244 ◽  
Author(s):  
K Torigoe ◽  
S Sato ◽  
H Kusaba ◽  
K Kohno ◽  
M Kuwano ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Gene Region ◽  
Multidrug Resistance Gene

Localization of 67 Exons on a YAC Contig Spanning 1.5 Mb around the Multidrug Resistance Gene Region of Human Chromosome 7q21.1

Genomics ◽  
1998 ◽  
Vol 49 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Kiyoyuki Torigoe ◽  
Taishi Harada ◽  
Hitoshi Kusaba ◽  
Takeshi Uchiumi ◽  
Kimitoshi Kohno ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Human Chromosome ◽  
Resistance Gene ◽  
Gene Region ◽  
Multidrug Resistance Gene ◽  
Yac Contig

Isolation and characterization of Drosophila multidrug resistance gene homologs

1991 ◽  
Vol 11 (8) ◽  
pp. 3940-3948
Author(s):  
C T Wu ◽  
M Budding ◽  
M S Griffin ◽  
J M Croop
Keyword(s):  
Amino Acid ◽  
Cell Lines ◽  
Multidrug Resistant ◽  
Amino Acid Identity ◽  
Resistant Cell ◽  
Cross Resistance ◽  
Multidrug Resistance Gene ◽  
Isolation And Characterization ◽  
P Glycoprotein

Mammalian multidrug-resistant cell lines, selected for resistance to a single cytotoxic agent, display cross-resistance to a broad spectrum of structurally and functionally unrelated compounds. These cell lines overproduce a membrane protein, the P-glycoprotein, which is encoded by a member(s) of a multigene family, termed mdr or pgp. The amino acid sequence of the P-glycoprotein predicts an energy-dependent transport protein with homology to a large superfamily of proteins which transport a wide variety of substances. This report describes the isolation and characterization of two Drosophila homologs of the mammalian mdr gene. These homologs, located in chromosomal sections 49EF and 65A, encode proteins that share over 40% amino acid identity to the human and murine mdr P-glycoproteins. Fly strains bearing disruptions in the homolog in section 49EF have been constructed and implicate this gene in conferring colchicine resistance to the organism. This work sets the foundation for the molecular and genetic analysis of mdr homologs in Drosophila melanogaster.

scholarly journals Enhancing the Therapeutic Efficacy of Daunorubicin and Mitoxantrone with Bavachinin, Candidone, and Tephrosin

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sina Darzi ◽  
Seyed Abbas Mirzaei ◽  
Fatemeh Elahian ◽  
Sadegh Shirian ◽  
Amir Peymani ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cancer Treatment ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell Line ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Dependent Manner ◽  
Expression Levels ◽  
Treatment Research

The capability of flavonoids in sensitizing cancer cells was demonstrated in numerous works to chemotherapy and converse multidrug resistance by modulating efflux pumps and apoptosis mechanisms. Three flavonoids, namely, bavachinin, tephrosin, and candidone, have been recently introduced to cancer treatment research presenting various activities, such as antibacterial, immunomodulatory, cell death, and anticancer. Less information exists regarding the therapeutic significance of these flavonoids in cancer treatment, especially in overcoming multidrug resistance (MDR). Here, we tempted to investigate the potency of these agents in reversing MDR by analyzing their effects as chemosensitizers on cell cytotoxicity, P-gp and ABCG2 protein expression levels, and their function on two multidrug-resistant cell lines, P-gp-overexpressing human gastric adenocarcinoma cell line (EPG85.257RDB) and ABCG2-overexpressing human epithelial breast cancer cell line (MCF7/MX). The inhibitory concentration of 10% (IC10) of bavachinin, tephrosin, and candidone in EPG85.257RDB cells was 1588.7 ± 202.2, 264.8 ± 86.15, and 1338.6 ± 114.11 nM, respectively. Moreover, these values in MCF7/MX cell were 2406.4 ± 257.63, 38.8 ± 4.28, and 27.9 ± 5.59 nM, respectively. Expression levels of ABCG2 and P-gp were not significantly downregulated by these flavonoids. Maximum levels of daunorubicin and mitoxantrone accumulations and minimum rates of drug efflux in both cell lines were detected 48 hrs posttreatment with tephrosin and bavachinin, respectively. Chemosensitization to mitoxantrone and daunorubicin treatments was, respectively, achieved in MCF7/MX and EPG85.257RDB cells in response to IC10 of bavachinin and tephrosin, independently. These effects did not follow time-dependent manner, and each flavonoid had its cell-dependent patterns. Overall, bavachinin, tephrosin, and candidone showed potency to sensitize MDR cells to daunorubicin and mitoxantrone and could be considered as attractive MDR modulators for cancer treatment. However, their action was time and cell specific.

scholarly journals Isolation and characterization of Drosophila multidrug resistance gene homologs.

1991 ◽  
Vol 11 (8) ◽  
pp. 3940-3948 ◽  
Author(s):  
C T Wu ◽  
M Budding ◽  
M S Griffin ◽  
J M Croop
Keyword(s):  
Amino Acid ◽  
Cell Lines ◽  
Multidrug Resistant ◽  
Amino Acid Identity ◽  
Resistant Cell ◽  
Cross Resistance ◽  
Multidrug Resistance Gene ◽  
Isolation And Characterization ◽  
P Glycoprotein

Mammalian multidrug-resistant cell lines, selected for resistance to a single cytotoxic agent, display cross-resistance to a broad spectrum of structurally and functionally unrelated compounds. These cell lines overproduce a membrane protein, the P-glycoprotein, which is encoded by a member(s) of a multigene family, termed mdr or pgp. The amino acid sequence of the P-glycoprotein predicts an energy-dependent transport protein with homology to a large superfamily of proteins which transport a wide variety of substances. This report describes the isolation and characterization of two Drosophila homologs of the mammalian mdr gene. These homologs, located in chromosomal sections 49EF and 65A, encode proteins that share over 40% amino acid identity to the human and murine mdr P-glycoproteins. Fly strains bearing disruptions in the homolog in section 49EF have been constructed and implicate this gene in conferring colchicine resistance to the organism. This work sets the foundation for the molecular and genetic analysis of mdr homologs in Drosophila melanogaster.

Cross-resistance to antifolates in multidrug resistant cell lines with P-glycoprotein or multidrug resistance protein expression

1997 ◽  
Vol 53 (12) ◽  
pp. 1855-1866 ◽  
Author(s):  
Baukelien van Triest ◽  
Herbert M. Pinedo ◽  
Frank Telleman ◽  
Clasina L. van der Wilt ◽  
Gerrit Jansen ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Protein Expression ◽  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Cross Resistance ◽  
Multidrug Resistance Protein ◽  
P Glycoprotein ◽  
Resistance Protein

Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

1992 ◽  
Vol 251 (2) ◽  
pp. 79-86 ◽  
Author(s):  
S. Sekiya ◽  
T. Nunoyama ◽  
H. Shirasawa ◽  
H. Kimura ◽  
M. Kawata ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Ovarian Carcinoma ◽  
Resistance Gene ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Multidrug Resistance Gene ◽  
Human Ovarian Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Ovarian Carcinoma

Expression of a Multidrug Resistance Gene in Blast Crisis of Chronic Myelogenous Leukemia

1989 ◽  
Vol 1 (2) ◽  
pp. 141-144 ◽  
Author(s):  
Robert Pirker ◽  
Lori J. Goldstein ◽  
Heinz Ludwig ◽  
Werner Linkesch ◽  
Christina Lechner ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Chronic Myelogenous Leukemia ◽  
Blast Crisis ◽  
Myelogenous Leukemia ◽  
Multidrug Resistance Gene
Sign in / Sign up

Export Citation Format

Share Document