scholarly journals Tobacco O-Methyltransferases Involved in Phenylpropanoid Metabolism. The Different Caffeoyl-Coenzyme A/5-Hydroxyferuloyl-Coenzyme A 3/5-O-Methyltransferase and Caffeic Acid/5-Hydroxyferulic Acid 3/5-O-Methyltransferase Classes Have Distinct Substrate Specificities and Expression Patterns

1999 ◽  
Vol 121 (1) ◽  
pp. 215-224 ◽  
Author(s):  
Stéphane Maury ◽  
Pierrette Geoffroy ◽  
Michel Legrand
Keyword(s):  
Caffeic Acid ◽  
Coenzyme A ◽  
Expression Patterns ◽  
Phenylpropanoid Metabolism ◽  
Substrate Specificities

scholarly journals Developmental Expression and Substrate Specificities of Alfalfa Caffeic Acid 3-O-Methyltransferase and Caffeoyl Coenzyme A 3-O-Methyltransferase in Relation to Lignification

1998 ◽  
Vol 117 (3) ◽  
pp. 761-770 ◽  
Author(s):  
Kentaro Inoue ◽  
Vincent J.H. Sewalt ◽  
G. Murray Ballance ◽  
Weiting Ni ◽  
Cornelia Stürzer ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Coenzyme A ◽  
Developmental Expression ◽  
Substrate Specificities

scholarly journals Genome-wide identification and characterization of caffeoyl-coenzyme A O-methyltransferase genes related to the Fusarium head blight response in wheat

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Guang Yang ◽  
Wenqiu Pan ◽  
Ruoyu Zhang ◽  
Yan Pan ◽  
Qifan Guo ◽  
...  
Keyword(s):  
Coenzyme A ◽  
Expression Patterns ◽  
Lignin Biosynthesis ◽  
Head Blight ◽  
Genome Wide ◽  
Genome Search ◽  
Gene Structures ◽  
Main Components ◽  
Main Regulator ◽  
Group Sharing

Abstract Background Lignin is one of the main components of the cell wall and is directly associated with plant development and defence mechanisms in plants, especially in response to Fusarium graminearum (Fg) infection. Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) is the main regulator determining the efficiency of lignin synthesis and composition. Although it has been characterized in many plants, to date, the importance of the CCoAOMT family in wheat is not well understood. Results Here, a total of 21 wheat CCoAOMT genes (TaCCoAOMT) were identified through an in silico genome search method and they were classified into four groups based on phylogenetic analysis, with the members of the same group sharing similar gene structures and conserved motif compositions. Furthermore, the expression patterns and co-expression network in which TaCCoAOMT is involved were comprehensively investigated using 48 RNA-seq samples from Fg infected and mock samples of 4 wheat genotypes. Combined with qRT-PCR validation of 11 Fg-responsive TaCCoAOMT genes, potential candidates involved in the FHB response and their regulation modules were preliminarily suggested. Additionally, we investigated the genetic diversity and main haplotypes of these CCoAOMT genes in bread wheat and its relative populations based on resequencing data. Conclusions This study identified and characterized the CCoAOMT family in wheat, which not only provided potential targets for further functional analysis, but also contributed to uncovering the mechanism of lignin biosynthesis and its role in FHB tolerance in wheat and beyond.

scholarly journals Characterization and functional analysis of two novel 3-hydroxy-3-methylglutaryl-coenzyme A reductase genes (GbHMGR2 and GbHMGR3) from Ginkgo biloba

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shen Rao ◽  
Xiangxiang Meng ◽  
Yongling Liao ◽  
Tian Yu ◽  
Jie Cao ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
High Performance ◽  
Coenzyme A ◽  
Expression Patterns ◽  
Mevalonic Acid ◽  
Regulatory Elements ◽  
Promoter Regions ◽  
Mva Pathway ◽  
Cdna Sequences ◽  
Coenzyme A Reductase

Abstract Terpene trilactones (TTLs) are the main secondary metabolites of Ginkgo biloba. As one of the rate-limiting enzymes in the mevalonic acid (MVA) pathway of TTL biosynthesis, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the 3-hydroxy-3-methylglutaryl coenzyme A to form MVA. In this study, two cDNA sequences of HMGR genes, namely, GbHMGR2 and GbHMGR3, were cloned from G. biloba. The protein sequences of GbHMGR2 and GbHMGR3, which contain several functional domains, were analyzed. Regulatory elements related to light, hormone, and stress response were detected in the promoter regions of GbHMGR2 and GbHMGR3. The catalytic activity of these genes was verified by a functional complement experiment in yeast. Quantitative real-time PCR (qRT-PCR) showed the distinct expression patterns of the two genes in different organs. The TTL contents in the organs were detected by high-performance liquid chromatography– evaporative light scattering detector. GbHMGR2 and GbHMGR3 were responded to cold, dark, methyl jasmonate (MJ), abscisic acid (ABA), salicylic acid (SA), and ethephon (Eth) treatments. The TTL contents were also regulated by cold, dark, MJ, ABA, SA, and Eth treatment. In conclusion, GbHMGR2 and GbHMGR3 may participate in the MVA pathway of TTL biosynthesis.

scholarly journals Substrate Specificities and Expression Patterns Reflect the Evolutionary Divergence of Maltose ABC Transporters in Thermotoga maritima

2005 ◽  
Vol 187 (6) ◽  
pp. 2002-2009 ◽  
Author(s):  
Dhaval M. Nanavati ◽  
Tu N. Nguyen ◽  
Kenneth M. Noll
Keyword(s):  
Guar Gum ◽  
Thermotoga Maritima ◽  
Expression Patterns ◽  
Regulatory Protein ◽  
Evolutionary Relationship ◽  
Growth Conditions ◽  
Evolutionary Divergence ◽  
Reading Frame ◽  
Substrate Specificities ◽  
Transcriptional Regulatory

ABSTRACT Duplication of transporter genes is apparent in the genome sequence of the hyperthermophilic bacterium Thermotoga maritima. The physiological impacts of these duplications are not well understood, so we used the bacterium's two putative maltose transporters to begin a study of the evolutionary relationship between a transporter's function and the control of expression of its genes. We show that the substrate binding proteins encoded by these operons, MalE1 and MalE2, have different substrate specificities and affinities and that they are expressed under different growth conditions. MalE1 binds maltose (dissociation constant [KD ], 24 ± 1 μM), maltotriose (KD , 8 ± 0.5 nM), and β-(1→4)-mannotetraose (KD , 38 ± 1 μM). In contrast, MalE2 binds maltose (KD , 8.4 ± 1 μM), maltotriose (KD , 11.5 ± 1.5 μM), and trehalose (KD , 9.5 ± 1.0 μM) confirming the findings of Wassenberg et al. (J. Mol. Biol. 295:279-288, 2000). Neither protein binds lactose. We examined the expression of these operons at both the transcriptional and translational levels and found that MalE1 is expressed in cells grown on lactose or guar gum and that MalE2 is highly expressed in starch- and trehalose-grown cells. Evidence is provided that malE1, malF1, and perhaps malG1 are cotranscribed and so constitute an operon. An open reading frame encoding a putative transcriptional regulatory protein adjacent to this operon (TM1200) is also up-regulated in response to growth on lactose. These evolutionarily related transporter operons have diverged both in function and expression to assume apparently different physiological roles.

scholarly journals Genome-wide identification and characterization of caffeoyl-coenzyme A O-methyltransferase genes related to Fusarium head blight response in wheat

2021 ◽  
Author(s):  
Guang Yang ◽  
Wenqiu Pan ◽  
Ruoyu Zhang ◽  
Yan Pan ◽  
Qifan Guo ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Defense Mechanisms ◽  
Coenzyme A ◽  
Expression Patterns ◽  
Lignin Biosynthesis ◽  
Head Blight ◽  
Gene Structures ◽  
Main Components ◽  
Main Regulator ◽  
And Control

Abstract Background Lignin is one of the main components of cell wall, which directly associates with the development and defense mechanisms in plants, especially in response to Fusarium head blight (FHB) tolerance. Caffeoyl-coenzyme A Omethyltransferase (CCoAOMT) is the main regulator determining the efficiency of lignin synthesis and composition. Although it has been widely characterized in many plants, the importance of CCoAOMT family in wheat is not well understood up to now. Results Here, a total 21 CCoAOMT genes were identified in wheat (TaCCoAOMT) through a in silico genome search method and they were classified into four groups based on phylogenetic analysis with the members in the same group sharing similar gene structures and conserved motif compositions. Furthermore, the expression patterns and co-expression network which these TaCCoAOMT involved in were comprehensively investigated using 48 RNA-seq samples from Fusarium graminearum-infected and control samples of 4 wheat genotypes. Combined with qRT-PCR validation of 11 Fg-responsive TaCCoAOMT genes, the potential candidates involving in FHB response and their regulation modules were preliminarily revealed. Additionally, we also investigated the genetic diversity and main haplotypes of these CCoAOMT genes in bread wheat and its relative populations based on resequencing data. Conclusion This study systematically identified and characterized the CCoAOMT gene family in wheat, which not only provided the targets for further functional analysis, but also contribute to the mechanism of lignin biosynthesis and its role in FHB tolerance in wheat and beyond.

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