Expression, purification and crystallization ofChaetomium thermophilumCu,Zn superoxide dismutase
2010 ◽
Vol 66
(9)
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pp. 1089-1092
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Keyword(s):
Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungusChaetomium thermophilumwas expressed inPichia pastorisand purified. Crystals were grown in over 120 conditions but only those produced with 1.4 Msodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 Å resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space groupP61/P65, with unit-cell parametersa= 90.2,c= 314.5 Å and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure–activity relationship, assembly and thermal stability of Cu,ZnSODs.
2015 ◽
Vol 71
(6)
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pp. 680-683
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1999 ◽
Vol 55
(8)
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pp. 1459-1461
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1999 ◽
Vol 55
(2)
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pp. 522-524
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2015 ◽
Vol 71
(3)
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pp. 261-265
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2014 ◽
Vol 70
(10)
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pp. 1424-1427
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1999 ◽
Vol 55
(9)
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pp. 1616-1617
2014 ◽
Vol 70
(6)
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pp. 765-768
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