Crystallization and preliminary X-ray diffraction analysis of human DNA primase
2014 ◽
Vol 70
(2)
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pp. 206-210
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Keyword(s):
X Ray
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Human primase synthesizes RNA primers and transfers them to the active site of Pol α with subsequent extension with dNTPs. Human primase is a heterodimer of two subunits: a small catalytic subunit (p49) and a large subunit (p58). The structural details of the initiation and elongation steps of primer synthesis, as well as primer length counting, are not known. To address these questions, structural studies of human primase were initiated. Two types of crystals were obtained. The best diffracting crystals belonged to space groupP1, with unit-cell parametersa= 86.2,b= 88.9,c= 94.68 Å, α = 93.82, β = 96.57, γ = 111.72°, and contained two heterodimers of full-length p49 and p59 subunits in the asymmetric unit.
1999 ◽
Vol 55
(9)
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pp. 1616-1617
2014 ◽
Vol 70
(6)
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pp. 777-780
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2000 ◽
Vol 56
(1)
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pp. 89-91
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2012 ◽
Vol 68
(2)
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pp. 190-192
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1999 ◽
Vol 55
(6)
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pp. 1231-1233
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