Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 fromStaphylococcus aureusMu50
2013 ◽
Vol 69
(4)
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pp. 405-407
Keyword(s):
X Ray
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SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistantStaphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space groupP3121, with unit-cell parametersa=b= 81.48,c= 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.
2010 ◽
Vol 66
(9)
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pp. 1050-1052
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2012 ◽
Vol 68
(11)
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pp. 1351-1353
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2014 ◽
Vol 70
(11)
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pp. 1560-1562
2015 ◽
Vol 71
(1)
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pp. 54-56
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1999 ◽
Vol 55
(6)
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pp. 1231-1233
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2015 ◽
Vol 71
(8)
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pp. 1109-1113
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