Observable Real-Time Pulsed-Field Gel Electrophoresis

2017 ◽  
Author(s):  
Deniz Ece Kaya ◽  
Tanıl Kocagöz ◽  
Sesin Kocagöz ◽  
Cengizhan Öztürk
Keyword(s):  
Real Time ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Use of real time pulsed field gel electrophoresis to guide interventions during a nursery outbreak of Serratia marcescens infection

1999 ◽  
Vol 18 (4) ◽  
pp. 357-360 ◽  
Author(s):  
CLAUDIA HOYEN ◽  
LOUIS RICE ◽  
SUSAN CONTE ◽  
MICHAEL R. JACOBS ◽  
MICHELE WALSH-SUKYS ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Real Time ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Application of Fourier transform infrared spectroscopy spectroscopy for real-time typing of Acinetobacter baumannii outbreak in intensive care unit

2021 ◽  
Author(s):  
Donatella Lombardo ◽  
Miriam Cordovana ◽  
Francesca Deidda ◽  
Marco Pane ◽  
Simone Ambretti
Keyword(s):  
Intensive Care ◽  
Acinetobacter Baumannii ◽  
Real Time ◽  
Gel Electrophoresis ◽  
Index Case ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Promising Tool ◽  
Detection And Tracking

Aim: Acinetobacter baumannii is a pathogen of serious concern, often exhibiting multiple antibiotic resistance, frequently associated with hospital outbreaks in intensive care units. A prompt detection and tracking of these isolates is crucial. Reference methods for typing (pulsed-field gel electrophoresis, whole-genome sequencing) are accurate, but expensive and time-consuming, therefore limited to retrospective analysis. Materials & methods: In this study, the application of the FTIR-based IR Biotyper® (IRBT) to track and monitor in real time the spread of a multidrug-resistant A. baumannii outbreak was investigated. The index case and the multidrug-resistant  A. baumannii isolates collected in the following 3 weeks were investigated. Results: IR Biotyper® clustering results were fully confirmed by pulsed-field gel electrophoresis results. Conclusions: IR Biotyper represent a promising tool for real-time hospital hygiene, enabling a prompt and reliable typing.

scholarly journals Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-Resistant Staphylococcus aureus Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 602-610
Author(s):  
Eugene Y. H. Yeung ◽  
Ivan Gorn
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pelvic Trauma ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Clinical Cases

Pulsed-field gel electrophoresis (PFGE) has historically been considered the gold standard in fingerprinting bacterial strains in epidemiological studies and outbreak investigations; little is known regarding its use in individual clinical cases. The current study detailed two clinical cases in which PFGE helped to determine the source of their methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Patient A was found to have MRSA bacteremia after trauma in her pelvic area. MRSA was also found in her groin but not in her nostril and rectum. PFGE was performed that showed variable bands of her MRSA isolates from blood and groin, suggestive of different strains of MRSA. Her MRSA bacteremia was determined to be unrelated to her pelvic trauma. Patient B was found to have MRSA bacteremia after colonoscopy. MRSA was also found in his nostril and rectum. PFGE was performed that showed variable bands of his MRSA isolates from blood and rectum but identical bands of MRSA isolates from his blood and nostril. His MRSA bacteremia was determined to be unrelated to his colonoscopy procedure. The current study demonstrates the use of PFGE to rule out the source of bacteremia in individual clinical cases.

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