Protecting Deep Cerebrospinal Fluid Cell Image Processing Models with Backdoor and Semi-Distillation

Author(s):  
Fang-Qi Li ◽  
Shi-Lin Wang ◽  
Zhen-Hai Wang
2021 ◽  
Vol 16 (2) ◽  
pp. 207-214
Author(s):  
Chi-Lu Chiang ◽  
Cheng-Chia Lee ◽  
Hsu-Ching Huang ◽  
Chia-Hung Wu ◽  
Yi-Chen Yeh ◽  
...  

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Zimeng Ye ◽  
Zac Chatterton ◽  
Jahnvi Pflueger ◽  
John A Damiano ◽  
Lara McQuillan ◽  
...  

Abstract Brain somatic mutations are an increasingly recognized cause of epilepsy, brain malformations and autism spectrum disorders and may be a hidden cause of other neurodevelopmental and neurodegenerative disorders. At present, brain mosaicism can be detected only in the rare situations of autopsy or brain biopsy. Liquid biopsy using cell-free DNA derived from cerebrospinal fluid has detected somatic mutations in malignant brain tumours. Here, we asked if cerebrospinal fluid liquid biopsy can be used to detect somatic mosaicism in non-malignant brain diseases. First, we reliably quantified cerebrospinal fluid cell-free DNA in 28 patients with focal epilepsy and 28 controls using droplet digital PCR. Then, in three patients we identified somatic mutations in cerebrospinal fluid: in one patient with subcortical band heterotopia the LIS1 p. Lys64* variant at 9.4% frequency; in a second patient with focal cortical dysplasia the TSC1 p. Phe581His*6 variant at 7.8% frequency; and in a third patient with ganglioglioma the BRAF p. Val600Glu variant at 3.2% frequency. To determine if cerebrospinal fluid cell-free DNA was brain-derived, whole-genome bisulphite sequencing was performed and brain-specific DNA methylation patterns were found to be significantly enriched (P = 0.03). Our proof of principle study shows that cerebrospinal fluid liquid biopsy is valuable in investigating mosaic neurological disorders where brain tissue is unavailable.


2017 ◽  
Vol 473 ◽  
pp. 133-138 ◽  
Author(s):  
Michela Seghezzi ◽  
Barbara Manenti ◽  
Giulia Previtali ◽  
Maria Grazia Alessio ◽  
Paola Dominoni ◽  
...  

2020 ◽  
Author(s):  
Mamata Anil Parab ◽  
Ninad Dileep Mehendale

AbstractIn the medical field, the analysis of the blood sample of the patient is a critical task. Abnormalities in blood cells are accountable for various health issues. Red blood cells (RBCs) are one of the major components of blood. Classifying the RBC can allow us to diagnose different diseases. The traditional time consuming technique of visualizing RBC manually under the microscope is a tedious task and may lead to wrong interpretation because of the human error. The various health conditions can change the shape, texture, and size of normal RBCs. The proposed method has involved the use of image processing to classify the RBCs with the help of Convolution Neural Networks (CNN). The algorithm can extract the feature of each segmented cell image and classify it in various types as Microcytes, Elliptocytes, Stomatocytes, Macrocytes, Teardrop RBCs, Codocytes, Spherocytes, Sickel cell RBCs and Howell jolly RBCs. Classification is done with respect to the size, shape, and appearance of RBCs. The experiment was conducted on the blood slide collected from the hospital and RBC images were extracted from those blood slide images. The obtained results compared with reports obtained by the pathology lab and realized 98.5% accuracy. The developed system provides accurate and fast results due to which it may save the life of patients.


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