Influence of the perfusion bioreactor on Stratified and Distributed approaches for multilayered tissue engineering on biodegradable scaffolds

2021 ◽  
Author(s):  
Kazutomo Baba ◽  
Andrey Mikhailov ◽  
Yoshiyuki Sankai
Keyword(s):  
Tissue Engineering ◽  
Perfusion Bioreactor ◽  
Biodegradable Scaffolds

Techniques and materials used to optimize attached gingiva size: literature review

2021 ◽  
Vol 1 (12) ◽  
pp. 42-47
Author(s):  
Z. S. Khabadze ◽  
A. B. Adzhieva ◽  
A. A. Nedashkovsky ◽  
Yu. A. Generalova ◽  
M. G. Sherozia ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Closure ◽  
Soft Tissue Defects ◽  
Autologous Tissue ◽  
Biodegradable Scaffolds ◽  
Attached Gingiva ◽  
Materials Used ◽  
Oral Soft Tissue ◽  
Tissue Defects ◽  
Interesting Alternative

The aim of this review was to examine the techniques for performing keratinized gingival augmentation and grafts, as well as the materials used, which are often required to ensure proper wound closure. Tissue engineering of the oral mucosa represents an interesting alternative to obtain sufficient autologous tissue to repair oral soft tissue defects using biodegradable scaffolds and can improve vascularization and epithelialization, which are critical for successful outcomes.

Development of a Perfusion Bioreactor System for Alveolar Bone Tissue Engineering

2009 ◽  
Author(s):  
Ki Taek Lim ◽  
Pill Hoon Choung ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
Hoon Seonwoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Perfusion Bioreactor ◽  
Bioreactor System

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

scholarly journals 3D printing biodegradable scaffolds with chitosan materials for tissue engineering

2018 ◽  
Vol 347 ◽  
pp. 012009 ◽  
Author(s):  
K N Bardakova ◽  
T S Demina ◽  
E A Grebenik ◽  
N V Minaev ◽  
T A Akopova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Biodegradable Scaffolds

scholarly journals A modular, standalone perfusion bioreactor for robust, monitored and controlled tissue engineering

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S81-S82
Author(s):  
S. de Bournonville ◽  
T. Lambrechts ◽  
J. Vanhulst ◽  
I. Papantoniou ◽  
L. Geris
Keyword(s):  
Tissue Engineering ◽  
Perfusion Bioreactor

scholarly journals Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 619 ◽  
Author(s):  
Jahangirian ◽  
Azizi ◽  
Rafiee-Moghaddam ◽  
Baratvand ◽  
Webster
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Review Paper ◽  
Wheat Gluten ◽  
Environmentally Friendly ◽  
Plant Proteins ◽  
Current Review ◽  
Biodegradable Scaffolds ◽  
New Strategy

In recent decades, regenerative medicine has merited substantial attention from scientific and research communities. One of the essential requirements for this new strategy in medicine is the production of biocompatible and biodegradable scaffolds with desirable geometric structures and mechanical properties. Despite such promise, it appears that regenerative medicine is the last field to embrace green, or environmentally-friendly, processes, as many traditional tissue engineering materials employ toxic solvents and polymers that are clearly not environmentally friendly. Scaffolds fabricated from plant proteins (for example, zein, soy protein, and wheat gluten), possess proper mechanical properties, remarkable biocompatibility and aqueous stability which make them appropriate green biomaterials for regenerative medicine applications. The use of plant-derived proteins in regenerative medicine has been especially inspired by green medicine, which is the use of environmentally friendly materials in medicine. In the current review paper, the literature is reviewed and summarized for the applicability of plant proteins as biopolymer materials for several green regenerative medicine and tissue engineering applications.

scholarly journals X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

2015 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Kenta Takashima ◽  
Masato Hoshino ◽  
Kentaro Uesugi ◽  
Naoto Yagi ◽  
Shojiro Matsuda ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
Tissue Engineering ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Injured Spinal Cord ◽  
X Ray ◽  
Biodegradable Scaffolds ◽  
Cord Injury ◽  
Grating Interferometer

Tissue engineering strategies for spinal cord repair are a primary focus of translational medicine after spinal cord injury (SCI). Many tissue engineering strategies employ three-dimensional scaffolds, which are made of biodegradable materials and have microstructure incorporated with viable cells and bioactive molecules to promote new tissue generation and functional recovery after SCI. It is therefore important to develop an imaging system that visualizes both the microstructure of three-dimensional scaffolds and their degradation process after SCI. Here, X-ray phase-contrast computed tomography imaging based on the Talbot grating interferometer is described and it is shown how it can visualize the polyglycolic acid scaffold, including its microfibres, after implantation into the injured spinal cord. Furthermore, X-ray phase-contrast computed tomography images revealed that degradation occurred from the end to the centre of the braided scaffold in the 28 days after implantation into the injured spinal cord. The present report provides the first demonstration of an imaging technique that visualizes both the microstructure and degradation of biodegradable scaffolds in SCI research. X-ray phase-contrast imaging based on the Talbot grating interferometer is a versatile technique that can be used for a broad range of preclinical applications in tissue engineering strategies.

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