A new VLSI algorithm for a high-throughput implementation of type IV DCT

2016 ◽  
Author(s):  
Doru Florin Chiper
Keyword(s):  
High Throughput ◽  
Type Iv ◽  
Vlsi Algorithm

scholarly journals High-Throughput Screen for Inhibitors of the Type IV Pilus Assembly ATPase PilB

mSphere ◽  
2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Keane J. Dye ◽  
Nancy J. Vogelaar ◽  
Pablo Sobrado ◽  
Zhaomin Yang
Keyword(s):  
High Throughput ◽  
Resistant Bacteria ◽  
High Throughput Screen ◽  
Type Iv Pilus ◽  
Antibiotic Resistant ◽  
Promising Alternative ◽  
Content Type ◽  
Type Iv ◽  
Compound Libraries

ABSTRACT The bacterial type IV pilus (T4P) is a prominent virulence factor in many significant human pathogens, some of which have become increasingly antibiotic resistant. Antivirulence chemotherapeutics are considered a promising alternative to antibiotics because they target the disease process instead of bacterial viability. However, a roadblock to the discovery of anti-T4P compounds is the lack of a high-throughput screen (HTS) that can be implemented relatively easily and economically. Here, we describe the first HTS for the identification of inhibitors specifically against the T4P assembly ATPase PilB in vitro. Chloracidobacterium thermophilum PilB (CtPilB) had been demonstrated to have robust ATPase activity and the ability to bind its expected ligands in vitro. We utilized CtPilB and MANT-ATP, a fluorescent ATP analog, to develop a binding assay and adapted it for an HTS. As a proof of principle, we performed a pilot screen with a small compound library of kinase inhibitors and identified quercetin as a PilB inhibitor in vitro. Using Myxococcus xanthus as a model bacterium, we found quercetin to reduce its T4P-dependent motility and T4P assembly in vivo. These results validated our HTS as effective in identifying PilB inhibitors. This assay may prove valuable in seeking leads for the development of antivirulence chemotherapeutics against PilB, an essential and universal component of all bacterial T4P systems. IMPORTANCE Many bacterial pathogens use their type IV pili (T4P) to facilitate and maintain infection of a human host. Small chemical compounds that inhibit the production or assembly of T4P hold promise in the treatment and prevention of infections, especially in the era of increasing threats from antibiotic-resistant bacteria. However, few chemicals are known to have inhibitory or anti-T4P activity. Their identification has not been easy due to the lack of a method for the screening of compound collections or libraries on a large scale. Here, we report the development of an assay that can be scaled up to screen compound libraries for inhibitors of a critical T4P assembly protein. We further demonstrate that it is feasible to use whole cells to examine potential inhibitors for their activity against T4P assembly in a bacterium.

scholarly journals A new VLSI algorithm and architecture for the hardware implementation of type IV discrete cosine transform using a pseudo-band correlation structure

2011 ◽  
Vol 1 (2) ◽  
Author(s):  
Doru Chiper
Keyword(s):  
Discrete Cosine Transform ◽  
Systolic Array ◽  
Correlation Structure ◽  
Efficient Design ◽  
Hardware Complexity ◽  
Cosine Transform ◽  
Type Iv ◽  
Vlsi Chip ◽  
Array Architecture ◽  
Vlsi Algorithm

AbstractA new VLSI algorithm and its associated systolic array architecture for a prime length type IV discrete cosine transform is presented. They represent the basis of an efficient design approach for deriving a linear systolic array architecture for type IV DCT. The proposed algorithm uses a regular computational structure called pseudoband correlation structure that is appropriate for a VLSI implementation. The proposed algorithm is then mapped onto a linear systolic array with a small number of I/O channels and low I/O bandwidth. The proposed architecture can be unified with that obtained for type IV DST due to a similar kernel. A highly efficient VLSI chip can be thus obtained with good performance in the architectural topology, computing parallelism, processing speed, hardware complexity and I/O costs similar to those obtained for circular correlation and cyclic convolution computational structures.

The LDE-Type Flare Occurrence (1969-1993)

1994 ◽  
Vol 144 ◽  
pp. 279-282
Author(s):  
A. Antalová
Keyword(s):  
Time Series ◽  
Fourier Analysis ◽  
Sunspot Cycle ◽  
List Type ◽  
Type Number ◽  
Solar Cycles ◽  
Type Iv ◽  
Long Duration ◽  
Cycle Maximum ◽  
Flare Index

AbstractThe occurrence of LDE-type flares in the last three cycles has been investigated. The Fourier analysis spectrum was calculated for the time series of the LDE-type flare occurrence during the 20-th, the 21-st and the rising part of the 22-nd cycle. LDE-type flares (Long Duration Events in SXR) are associated with the interplanetary protons (SEP and STIP as well), energized coronal archs and radio type IV emission. Generally, in all the cycles considered, LDE-type flares mainly originated during a 6-year interval of the respective cycle (2 years before and 4 years after the sunspot cycle maximum). The following significant periodicities were found:• in the 20-th cycle: 1.4, 2.1, 2.9, 4.0, 10.7 and 54.2 of month,• in the 21-st cycle: 1.2, 1.6, 2.8, 4.9, 7.8 and 44.5 of month,• in the 22-nd cycle, till March 1992: 1.4, 1.8, 2.4, 7.2, 8.7, 11.8 and 29.1 of month,• in all interval (1969-1992):a)the longer periodicities: 232.1, 121.1 (the dominant at 10.1 of year), 80.7, 61.9 and 25.6 of month,b)the shorter periodicities: 4.7, 5.0, 6.8, 7.9, 9.1, 15.8 and 20.4 of month.Fourier analysis of the LDE-type flare index (FI) yields significant peaks at 2.3 - 2.9 months and 4.2 - 4.9 months. These short periodicities correspond remarkably in the all three last solar cycles. The larger periodicities are different in respective cycles.

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