Statistical features of REMA data of antimycobacterial drug screening and determining the minimal inhibitory concentration

Author(s):  
Eugene B. Postnikov ◽  
Anastasia I. Lavrova
Author(s):  
Lê Văn Bảo Duy ◽  
Dương Thị Thủy ◽  
Nguyễn Ngọc Phước ◽  
Trương Thị Hoa ◽  
Nguyễn Đức Quỳnh Anh

Nghiên cứu được tiến hành nhằm xác định nồng độ ức chế tối thiểu (Minimal Inhibitory Concentration - MIC) của một số loại kháng sinh đến vi khuẩn phân lập được từ cá dìa thương phẩm mắc bệnh lở loét (Siganus guttatus). Từ kết quả phân lập định danh cho thấy 2 chủng Vibrio parahaemolyticus VPMP22 và Vibrio tubiashii ATCC 19109 có mặt trên các vết lở loét ở cá dìa thương phẩm. Kết quả thử nghiệm MIC cho thấy các loại kháng sinh Cefuroxim, Cefotaxim, Tetracycline, Erythromicin, Rifamicin có nồng độ ức chế vi khuẩn Vibrio parahaemolyticus VPMP22 tốt nhất dưới 0.21 µg/ml. Các kháng sinh có Cefuroxim, Cefotaxim, Oxytetraciline, Erythromicin, Trimethoprim nồng độ ức chế vi khuẩn Vibrio tubiashii ATCC 19109 tốt nhất dưới 1.25 µg/ml. Penicillin có nồng độ ức chế tối thiểu cao nhất đối với cả 2 chủng vi khuẩn trên (80 µg/ml), cho thấy 2 chủng vi khuẩn trên đã có sự kháng thuốc đối với loại kháng sinh này. Do đó, trong phòng trị bệnh lở loét trên cá dìa nên sử dụng Cefuroxim và Cefotaxim để có hiệu quả cao nhất trong phòng trị bệnh.


1993 ◽  
Vol 21 (2) ◽  
pp. 151-155
Author(s):  
Gustaw Kerszman

The toxicity of the first ten MEIC chemicals to Escherichia coli and Bacillus subtilis was examined. Nine of the chemicals were toxic to the bacteria, with the minimal inhibitory concentration (MIC) ranging from 10-3 to 4.4M. The sensitivities of both organisms were similar, but the effect on E. coli was often bactericidal, while it was bacteriostatic for B. subtilis. Digoxin was not detectably toxic to either bacterial species. Amitriptyline and FeSO4 were relatively less toxic to the bacteria than to human cells. For seven chemicals, a highly significant linear regression was established between log MIC in bacteria and log of blood concentration, giving lethal and moderate/mild toxicity in humans, as well as with toxicity to human lymphocytes.


1985 ◽  
Vol 31 (8) ◽  
pp. 707-710 ◽  
Author(s):  
Herman Gershon ◽  
Anthony T. Grefig ◽  
David J. Cady

The copper(II) contents of the growth media, Sabouraud dextrose and Czapek–Dox broths, and of the spore inocula of Aspergillus niger (ATCC 1004), Aspergillus oryzae (ATCC 1011), Trichoderma viride (ATCC 8678), and Myrothecium verrucaria (ATCC 9095) were determined by atomic absorption spectrophotometry in a graphite furnace. The test systems composed of Sabouraud dextrose broth and spore inocula of the four fungi contained only a little over 3% of the copper(II) required to form a minimal inhibitory concentration of bis(8-quinolinolato)copper(II). The test system of Czapek–Dox broth and A. oryzae contained slightly less than 65% of the copper(II) required to form a minimal inhibitory concentration of the bischelate of 8-quinolinol with copper(II). When the minimal inhibitory concentrations of 8-quinolinol and bis(8-quinolinolato)copper(II) were added simultaneously to the test system of Czapek–Dox broth and A. oryzae, 10% of the combined mixture of toxicants caused complete inhibition of growth indicating synergism between the toxicants. These results together with the observation that α-lipoic acid as well as small aliphatic thiol-containing compounds (cysteine, glutathione, dithioerythritol, and dithiothreitol) reversed the toxicity of 8-quinolinol but not the toxicity of bis(8-quinolinolato)copper(II) led to the conclusion that the mechanisms of fungitoxicity of both toxicants are different.


1983 ◽  
Vol 38 (1-2) ◽  
pp. 151-152 ◽  
Author(s):  
Ferdinand Devínsky ◽  
Ivan Lacko ◽  
Ludovít Krasnec ◽  
Dušan Mlynarčík

Antimicrobial activity of N,N′-bis(decylmethyl)-α,ω-alkanediamine dioxides determined on Staphylococcus aureus, Escherichia coli, and Candida albicans is presented as minimal inhibitory concentration (MIC). The effect of the length of linking alkylene chain on this activity has been followed.


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