The effect of electric field on sichled red blood cells

AbstractLiposomes are widely assumed to present a straightforward physical model of cells. However, almost all previous liposome experiments with pulsed electric fields (PEFs) have been conducted in low-conductivity liquids, a condition that differs significantly from that of cells in medium. Here, we prepared liposomes consisting of soy bean lecithin and cholesterol, at a molar ratio of 1:1, in higher-conductivity liquid that approximated the conditions of red blood cells in phosphate-buffered saline, with inner and outer liquid conductivities of 0.6 and 1.6 S/m, respectively. We found that a single 1.1 kV/cm, 400 μs PEF promoted cell-like spontaneous division of liposomes.

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Effects of the weak alternating electric field on Na+ concentration inside human red blood cells

Bioelectrochemistry and Bioenergetics ◽

10.1016/s0302-4598(98)00065-8 ◽

1998 ◽

Vol 45 (1) ◽

pp. 127-130 ◽

Cited By ~ 3

Author(s):

Gang Chen ◽

Guoping Cai ◽

Riqing Zhang ◽

Pingguan Tu ◽

Nanming Zhao

Keyword(s):

Electric Field ◽

Red Blood Cells ◽

Blood Cells ◽

Human Red Blood Cells ◽

Alternating Electric Field

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The Electrophoretie Mobility of Red Blood Cells of Normal Human Beings

Blood ◽

10.1182/blood.v17.1.119.119 ◽

1961 ◽

Vol 17 (1) ◽

pp. 119-124 ◽

Cited By ~ 10

Author(s):

JOHN ANGERS ◽

ANTONIO ROTTINO

Keyword(s):

Electric Field ◽

Red Blood Cells ◽

Blood Cells ◽

Human Beings ◽

Healthy Individuals ◽

Disease States ◽

Electrophoretic Behavior ◽

Normal Human ◽

Healthy Persons

Abstract Values obtained for the electrophoretic behavior of the red blood cells of healthy individuals is presented. The technic and instrument are described in detail and the necessary attention to meticulous care is emphasized. The data presented show that in an electric field the mobility of the red blood cells of healthy persons is constant and reproducible. It was concluded that the method is extremely sensitive and precise and that it may prove of value in the study of various disease states.

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Membrane Fusion and Deformation of Red Blood Cells by Electric Fields

Zeitschrift für Naturforschung C ◽

10.1515/znc-1980-11-1236 ◽

1980 ◽

Vol 35 (11-12) ◽

pp. 1081-1085 ◽

Cited By ~ 31

Author(s):

Peter Scheurich ◽

Ulrich Zimmermann ◽

Maja Mischel ◽

Ingolf Lamprecht

Keyword(s):

Electric Field ◽

Red Blood Cells ◽

Membrane Fusion ◽

Electric Fields ◽

Blood Cells ◽

Electrical Breakdown ◽

Osmotic Shock ◽

Sine Wave ◽

Hypotonic Solution ◽

Alternating Electric Field

Abstract Human red blood cells suspended in a slightly hypotonic solution of low electric conductivity were exposed to an inhomogeneous and alternating electric field (sine wave, 30 V peak-to-peak value, electrode distance 120 μm, 0.5 to 2 MHz). Due to the dielectrophoretic effect the cells align parallel to the field lines under the formation of pearl chains. At high voltages (10 V amplitude) membrane fusion is observed between the adhered red blood cells in the pearl chains, whereby the chains become attached to the electrodes. In contrast to the pearl chains observed at voltages of up to 5 V amplitude the resulting fused and uniform aggregates which exhibit no recognisable individual cells under the light microscope, remain stable, even after the alternating electric field has been switched off or after haemolysis in response to osmotic shock. The fused aggregates are highly elastic. If the field strength of the applied alternating electric field is further increased they are stretched in the direction of the opposite electrode. Frequently, bridges are formed between the two electrodes. The uniform bridges remain stable for some time even in the absence of an electric field. The possibility of cell fusion and its initiation by electrical breakdown of the cell membranes are discussed.

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