Product modeling methods in collaborative engineering environments

Author(s):  
L. Horvath ◽  
T. Varga
Author(s):  
D.J. Benefiel ◽  
R.S. Weinstein

Intramembrane particles (IMP or MAP) are components of most biomembranes. They are visualized by freeze-fracture electron microscopy, and they probably represent replicas of integral membrane proteins. The presence of MAP in biomembranes has been extensively investigated but their detailed ultrastructure has been largely ignored. In this study, we have attempted to lay groundwork for a systematic evaluation of MAP ultrastructure. Using mathematical modeling methods, we have simulated the electron optical appearances of idealized globular proteins as they might be expected to appear in replicas under defined conditions. By comparing these images with the apearances of MAPs in replicas, we have attempted to evaluate dimensional and shape distortions that may be introduced by the freeze-fracture technique and further to deduce the actual shapes of integral membrane proteins from their freezefracture images.


Author(s):  
Cristian Iorga ◽  
Alain Desrochers

The expansion of the markets corroborated with product customization and short time to launch the product have led to new levels of competition among product development companies. To be successful in the globalization of the markets and to enable the evaluation and validation of products, companies have to develop methodologies focused on lifecycle analysis and reduction of product variation to obtain both quality and robustness of products. Keywords: Modeling, Evaluation, Validation, Design ProcessThis paper proposes a new design process methodology that unifies theoretical results of modeling stage and empirical findings obtained from the validation stage. The evaluations and validations of engineering design are very important and they have a high influence on product performances and their functionality, as well on the customer perceptions.Given that most companies maintain the confidentiality of their product development processes and that the existing literature does not provide more detailed aspects of this field, the proposed methodology will represent a technical and logistical support intended for students or engineers involved in academic as well as industrial projects.A generic methodology will be refined based on a new approach that will take into consideration the specification types (quantitative or qualitative), the design objectives and the product types: new/improved, structural/esthetic. Hence the new generic methodology will be composed of specific product validation algorithms taking into account the above considerations. At the end of this paper, the improvements provided by the proposed methodology into the design process will be shown in the context of the engineering student capstone projects at the Université de Sherbrooke.


Author(s):  
N. K. Smagulov ◽  
A. A. Adilbekova ◽  
N. V. Gitenis

The use of mathematical modeling methods allowed us to develop criteria for functional stress for assessing and predicting the level of functional stress of foreign students in the process of adaptation to the educational process.


Author(s):  
D. A. Belinskaya ◽  
Yu. V. Chelusnova ◽  
V. V. Abzianidze ◽  
N. V. Goncharov

Poisoning with organophosphorus compounds occupy one of the leading places in exotoxicosis. At the first stage, the detoxification of organophosphates can be provided with the help of DNA or RNA aptamers that bind the poison in the bloodstream. Currently, the main method of searching for aptamers is the experimental method of systematic evolution of ligands by exponential enrichment (SELEX). In the process of aptamer selection, the target molecule must be immobilized via the streptavidin-biotin complex. Since the poison molecule is small in size, to increase its availability for binding to aptamer, it is necessary to use a spacer between organophosphorus compounds and biotin. The aim of this work was to optimize the selection of aptamers for organophosphorus compounds by increasing the availability of a poison molecule immobilized via the streptavidin-biotin complex on the example of paraoxon. For this purpose, three spacers between organophosphorus compounds and biotin were tested using molecular modeling methods: three links of polyethylene glycol (3-PEG), four links of polyethylene glycol (4-PEG) and aminohexyl. The conformation of the biotinylated paraoxon complex with streptavidin and the interaction of paraoxon with the binding fragment of the aptamer were modeled using molecular docking and molecular dynamics methods. The ability of biotinylated paraoxon to bind to the aptamer has been evaluated by analyzing the surface area of the paraoxon available to the solvent, as well as by calculating the free binding energies. It has been shown that only in the case of aminohexyl immobilized paraoxon can contact the aptamer. At the final stage, the synthesis of paraoxon bound to biotin via aminohexyl was carried out.


Author(s):  
Balazs Balogh ◽  
Anna Carbone ◽  
Virginia Spanò ◽  
Alessandra Montalbano ◽  
Paola Barraja ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document