Extracted Passiflora edulis Pulp to Reduce Inflammation in LPS-activated Macrophage Cell Line: RAW 264.7

Herein, we provide the Raman imaging results for different stages of erythrophagocytosis of senescent red blood cells executed by isolated murine primary Kupffer cells and a murine macrophage cell line (RAW 264.7).

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Radiofrequency at 2.45 GHz increases toxicity, pro-inflammatory and pre-apoptotic activity caused by black carbon in the RAW 264.7 macrophage cell line

The Science of The Total Environment ◽

10.1016/j.scitotenv.2020.142681 ◽

2020 ◽

pp. 142681

Author(s):

Rosa Ana Sueiro-Benavides ◽

Jose Manuel Leiro-Vidal ◽

Aarón Ángel Salas-Sánchez ◽

J. Antonio Rodríguez-González ◽

Francisco J. Ares-Pena ◽

...

Keyword(s):

Cell Line ◽

Black Carbon ◽

Raw 264.7 ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Raw 264.7 Macrophage ◽

Apoptotic Activity

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NF-κB- and c-Jun-dependent regulation of human cytomegalovirus immediate-early gene enhancer/promoter in response to lipopolysaccharide and bacterial CpG-oligodeoxynucleotides in macrophage cell line RAW 264.7

European Journal of Biochemistry ◽

10.1111/j.1432-1033.2004.04011.x ◽

2004 ◽

Vol 271 (6) ◽

pp. 1094-1105 ◽

Cited By ~ 48

Author(s):

Younghee Lee ◽

Wern-Joo Sohn ◽

Doo-Sik Kim ◽

Hyung-Joo Kwon

Keyword(s):

Cell Line ◽

Human Cytomegalovirus ◽

Immediate Early Gene ◽

Early Gene ◽

Immediate Early ◽

Raw 264.7 ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Cpg Oligodeoxynucleotides ◽

Gene Enhancer

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Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7

Infection and Immunity ◽

10.1128/iai.69.5.3214-3223.2001 ◽

2001 ◽

Vol 69 (5) ◽

pp. 3214-3223 ◽

Cited By ~ 12

Author(s):

Xiaohan Du ◽

Martin G. Low

Keyword(s):

Oxidative Stress ◽

Inflammatory Response ◽

Cell Line ◽

Phospholipase D ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Down Regulation ◽

Tnf Α

ABSTRACT Serum glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) activity is reduced over 75% in systemic inflammatory response syndrome. To investigate the mechanism of this response, expression of the GPI-PLD gene was studied in the mouse monocyte-macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS; 0.5 to 50 ng/ml). GPI-PLD mRNA was reduced approximately 60% in a time- and dose-dependent manner. Oxidative stress induced by 0.5 mM H2O2 or 50 μM menadione also caused a greater than 50% reduction in GPI-PLD mRNA. The antioxidant N-acetyl-l-cysteine attenuated the down-regulatory effect of H2O2but not of LPS. Cotreatment of the cells with actinomycin D inhibited down-regulation induced by either LPS or H2O2. The half-life of GPI-PLD mRNA was not affected by LPS, or decreased slightly with H2O2, indicating that the reduction in GPI-PLD mRNA is due primarily to transcriptional regulation. Stimulation with tumor necrosis factor alpha (TNF-α) resulted in ∼40% reduction in GPI-PLD mRNA in human A549 alveolar carcinoma cells but not RAW 264.7 cells, suggesting that alternative pathways could exist in different cell types for down-regulating GPI-PLD expression during an inflammatory response and the TNF-α autocrine signaling mechanism alone is not sufficient to recapitulate the LPS-induced reduction of GPI-PLD in macrophages. Sublines of RAW 264.7 cells with reduced GPI-PLD expression exhibited increased cell sensitivity to LPS stimulation and membrane-anchored CD14 expression on the cell surface. Our data suggest that down-regulation of GPI-PLD could play an important role in the control of proinflammatory responses.

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