Cytotoxicity to Melanoma and Proliferation to Fibroblasts of Cold Plasma Treated Solutions With Removal of Hydrogen Peroxide and Superoxide Anion

2019 ◽  
Vol 47 (10) ◽  
pp. 4664-4669
Author(s):  
Zhuqing Feng ◽  
Zhao Xu ◽  
SiChuan Pu ◽  
XingMin Shi ◽  
Yun Yang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Anion ◽  
Cold Plasma

Oxidation of hydrogen peroxide at the dropping mercury electrode

1984 ◽  
Vol 49 (10) ◽  
pp. 2320-2331 ◽  
Author(s):  
Miroslav Březina ◽  
Martin Wedell
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Anion ◽  
Ph Value ◽  
Mercury Electrode ◽  
Electrode Process ◽  
Electrochemical Processes ◽  
Dropping Mercury Electrode ◽  
Oxidation Of Hydrogen ◽  
Decreasing Ph ◽  
Rate Controlling Step

Reduction of oxygen and oxidation of hydrogen peroxide at the dropping mercury electrode are electrochemical processes strongly influenced both by the pH value and the anions in solution. With decreasing pH, both processes become irreversible, especially in the presence of anions with a negative φ2 potential of the diffusion part of the double layer. In the case of irreversible oxygen reduction, the concept that the rate-controlling step of the electrode process is the acceptance of the first electron with the formation of the superoxide anion, O2-, was substantiated. Oxidation of hydrogen peroxide becomes irreversible at a lower pH value than the reduction of oxygen. The slowest, i.e. rate-controlling step of the electrode process in borate buffers at pH 9-10 is the transfer of the second electron, i.e. oxidation of superoxide to oxygen.

Factors which affect DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate

1982 ◽  
Vol 60 (11) ◽  
pp. 1359-1366 ◽  
Author(s):  
H. C. Birnboim
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Phorbol Myristate Acetate ◽  
Respiratory Burst ◽  
Superoxide Anion ◽  
Tumor Promoter ◽  
Myristate Acetate ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage

We have recently reported that phorbol myristate acetate (PMA) induces extensive DNA strand break damage in human peripheral blood leukocytes. The mechanism of action involves superoxide anion and hydrogen peroxide which are generated by phagocytes during the "respiratory burst." In this report, we describe the effect of various inhibitors and scavengers on PMA-induced DNA damage. Azide and cyanide greatly increased the level of damage; sulfhydryl compounds (glutathione, cysteine, and cysteamine) and ascorbate markedly decreased the level of damage. Hydroxyl radical scavengers such as dimethyl sulfoxide (DMSO) and glycerol also decreased the level of damage but apparently did so by inhibiting the respiratory burst. Diethyldithiocarbamate (DDC) increased the level of DNA damage at low concentrations (<1 mM), but decreased DNA damage at ≥1 mM. The results are consistent with a mechanism involving superoxide anion and hydrogen peroxide, but the precise reaction (free radical or enzymatic) responsible for DNA strand breakage has not been determined. The PMA-stimulated phagocyte is an interesting model system for looking at "active oxygen" mediated DNA damage and factors which influence it.

scholarly journals Macrophage variants in oxygen metabolism.

1980 ◽  
Vol 152 (4) ◽  
pp. 808-822 ◽  
Author(s):  
G Damiani ◽  
C Kiyotaki ◽  
W Soeller ◽  
M Sasada ◽  
J Peisach ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cytochrome C ◽  
Superoxide Anion ◽  
Oxygen Metabolism ◽  
Hexose Monophosphate ◽  
Phagocytic Cells ◽  
Form Complex ◽  
Hexose Monophosphate Shunt ◽  
Cytochrome C Reduction ◽  
Generate Superoxide Anion

Whereas phagocytic cells from normal individuals have the capacity to kill ingested bacteria and parasites, those from patients with several uncommon genetic deficiency diseases are known to be defective in bactericidal activity. Studies on neutrophils of these patients have revealed fundamental defects in their ability to reduce molecular oxygen and metabolize it to superoxide anion, hydrogen peroxide, and oxygen radicals. In the present experiments, we describe a clone of a continuous murine macrophage-like cell line, J774.16, that, upon appropriate stimulation, activates the hexose monophosphate shunt, and produces superoxide anion and hydrogen peroxide. With nitroblue tetrazolium to select against cells capable of being stimulated by phorbol myristate acetate to reduce the dye to polymer--formazan--which is toxic fot cells, we have selected for variants that are defective in oxygen metabolism. Four of these subclones have been characterized and found to be lacking in the ability (a) to generate superoxide anion, as measured by cytochrome c reduction; (b) to produce hydrogen peroxide, as measured by the ability to form complex I with cytochrome c peroxidase; and (c) to be stimulated to oxidize glucose via the hexose monophosphate shunt. These variants appear to represent a useful model for studying the molecular basis for macrophage cytocidal activity.

scholarly journals Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis

2004 ◽  
Vol 17 (3) ◽  
pp. 304-312 ◽  
Author(s):  
Marco Trujillo ◽  
Karl-Heinz Kogel ◽  
Ralph Hückelhoven
Keyword(s):  
Hydrogen Peroxide ◽  
Host Defense ◽  
Superoxide Anion ◽  
Penetration Resistance ◽  
Blumeria Graminis ◽  
Microscopic Level ◽  
Hypersensitive Cell Death ◽  
Formae Speciales ◽  
Cell Death Response ◽  
Wall Appositions

Nonhost resistance of cereals to inappropriate formae speciales of Blumeria graminis is little understood. However, on the microscopic level, nonhost defense to B. graminis is reminiscent of host defense preventing fungal development by penetration resistance and the hypersensitive cell death response (HR). We analyzed histochemically the accumulation of superoxide anion radicals (O2•¯) and hydrogen peroxide (H2O2) at sites of B. graminis attack in nonhost barley and wheat. Superoxide visualized by subcellular reduction of nitroblue tetrazolium accumulated in association with successful fungal penetration in attacked cells and in cells neighboring HR. In contrast, H2O2 accumulated in cell wall appositions beneath fungal penetration attempts or in the entire epidermal cell during HR. The data provide evidence for different roles and sources of superoxide and H2O2 in the nonhost interaction of cereals with inappropriate formae speciales of B. graminis.

scholarly journals Involvement of an NAD(P)H oxidase-like enzyme in superoxide anion and hydrogen peroxide generation by rat type II cells

Thorax ◽  
1997 ◽  
Vol 52 (5) ◽  
pp. 465-471 ◽  
Author(s):  
R. J. van Klaveren ◽  
C. Roelant ◽  
M. Boogaerts ◽  
M. Demedts ◽  
B. Nemery
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Anion ◽  
Type Ii Cells ◽  
Type Ii ◽  
Hydrogen Peroxide Generation
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