Molecular detection and characterisation of black raspberry necrosis virus and raspberry bushy dwarf virus isolates in wild raspberries

2018 ◽  
Vol 173 (2) ◽  
pp. 97-111 ◽  
Author(s):  
H. Susi ◽  
M.L. Rajamäki ◽  
K. Artola ◽  
F.R. Jayaraj-Mallika ◽  
J.P.T. Valkonen
Keyword(s):  
Molecular Detection ◽  
Dwarf Virus ◽  
Black Raspberry ◽  
Necrosis Virus ◽  
Raspberry Bushy Dwarf Virus ◽  
Virus Isolates

scholarly journals First Report of Raspberry bushy dwarf virus in Rubus multibracteatus from China

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 603-603 ◽  
Author(s):  
C. J. Chamberlain ◽  
J. Kraus ◽  
P. D. Kohnen ◽  
C. E. Finn ◽  
R. R. Martin
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Black Raspberry ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Plant Pathol

Raspberry bushy dwarf virus (RBDV), genus Idaeovirus, has been reported in commercial Rubus spp. from North and South America, Europe, Australia, New Zealand, and South Africa. Infection can cause reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields (3,4). In recent years, Rubus germplasm in the form of seed, was obtained on several collection trips to The People's Republic of China to increase the diversity of Rubus spp. in the USDA-ARS National Clonal Germplasm Repository, (Corvallis, OR). Before planting in the field, seedlings were tested for the presence of RBDV, Tomato ringspot virus, and Tobacco streak virus using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) (antiserum produced by R. R. Martin). One symptomless plant of R. multibracteatus H. Lev. & Vaniot (PI 618457 in USDA-ARS GRIN database), from Guizhou province in China, tested positive for RBDV (RBDV-China). After mechanical transmission on Chenopodium quinoa Willd., this isolate produced typical symptoms of RBDV (3). To determine if RBDV-China was a contaminant during the handling of the plants, or if the source was a seedborne virus, the coat protein gene was sequenced and compared to published sequences of RBDV. RNA was extracted from leaves of R. multibracteatus and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers that flank the coat protein gene. Products from four separate PCR reactions were sequenced directly or were cloned into the plasmid vector pCR 2.1 (Invitrogen, Carlsbad, CA) and then sequenced. The coding sequence of the coat protein gene of RBDV-China was 87.5% (722/825) identical to that isolated from black raspberry (Genbank Accession No. s55890). The predicted amino acid sequences were 91.6% (251/274) identical. Previously, a maximum of five amino acid differences had been observed in the coat proteins of different RBDV strains (1). The 23 differences observed between RBDV-China and the isolate from black raspberry (s55890) confirm that the RBDV in R. multibracteatus is not a greenhouse contaminant but is indeed a unique strain of RBDV. In addition, monoclonal antibodies (MAbs) to RBDV (2) were tested against RBDV-China. In these tests, MAb D1 did not detect RBDV-China, whereas MAb R2 and R5 were able to detect the strain. This is the first strain of RBDV that has been clearly differentiated by MAbs using standard TAS-ELISA tests. Although RBDV is common in commercial Rubus spp. worldwide, to our knowledge, this is the first report of RBDV in R. multibracteatus, and the first report of RBDV from China. The effects of this new strain of RBDV could be more or less severe, or have a different host range than previously studied strains. It is more divergent from the type isolate than any other strain that has been studied to date. Phylogenetic analysis of coat protein genes of RBDV may be useful in understanding the evolution and spread of this virus. References: (1) A. T. Jones et al. Eur. J. Plant Pathol. 106:623, 2000. (2) R. R. Martin. Can. J. Plant. Pathol. 6:264, 1984. (3) A. F. Murant. Raspberry Bushy Dwarf. Page 229 in: Virus Diseases of Small Fruits. R. H. Converse, ed. U.S. Dep. Agric. Agric. Handb. 631, 1987. (4) B. Strik and R. R. Martin. Plant Dis. 87:294, 2003.

scholarly journals First Report of Black raspberry necrosis virus in Rubus canadensis in Tennessee

Plant Disease ◽  
2008 ◽  
Vol 92 (8) ◽  
pp. 1254-1254 ◽  
Author(s):  
S. Sabanadzovic ◽  
N. Abou Ghanem-Sabanadzovic
Keyword(s):  
Ringspot Virus ◽  
Dwarf Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Cdna Clones ◽  
Black Raspberry ◽  
Necrosis Virus ◽  
Reading Frame ◽  
First Report ◽  
Biodiversity Inventory ◽  
Satsuma Dwarf Virus

Symptoms that resembled virus infection were observed on several smooth blackberry (Rubus canadensis L.) plants in the Great Smoky Mountain National Park (GSMNP) during a project carried out in the summer and fall of 2006 as part of All Taxa Biodiversity Inventory (ATBI) activities (2). Diseased specimens showed chlorosis and mottling as well as deformation of younger leaves. Symptomatic leaves were collected, and preliminary screening by double-antibody sandwich (DAS)-ELISA did not detect Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), Tomato spotted wilt virus (TSWV), Raspberry bushy dwarf virus (RBDV), or Impatiens necrotic spot virus (INSV) in these samples. Double-stranded RNA (dsRNA) extracted from symptomatic leaves of two diseased specimens (GSM-1 and GSM-2) revealed two bands with sizes estimated at 7.5 and 6.5 kb. Purified dsRNAs from specimen GSM-1 were used as a template to generate random-primed cDNA clones. Several clones were sequenced and analysis of approximately 3 kb of contiguous sequence (GenBank Accession No. EU419645) revealed the presence of a single open reading frame encoding a protein containing the complete proteinase and partial polymerase domains. BLAST analysis showed that the virus from R. canadensis shared 77 and 87% nucleotide and amino acid sequence identity, respectively, with the recently described putative sadwavirus Black raspberry necrosis virus (BRNV) (GenBank Accession No. NC_008182) (1), suggesting that this virus is a distinct isolate of BRNV. The virus shared a lower degree of identity with the analogous sequences of other viruses belonging to the genus Sadwavirus, with only 48 and 37% identity with Strawberry mottle virus (GenBank Accession No. NC_003445) and Satsuma dwarf virus (GenBank Accession No. NC_003785), respectively. To our knowledge, this is the first report of BRNV in GSMNP and Tennessee, indicating that it may be widespread in native Rubus spp. through the southeastern United States. References: (1) A. Halgren et al. Phytopathology 97:44, 2007. (2) M. J. Sharkey. Fla. Entomol. 84:556, 2001.

First Report of Raspberry bushy dwarf virus in Ohio

2005 ◽  
Vol 6 (1) ◽  
pp. 30 ◽  
Author(s):  
M. A. Ellis ◽  
J. Kraus ◽  
R. R. Martin ◽  
S. R. Wright
Keyword(s):  
Ringspot Virus ◽  
Dwarf Virus ◽  
Black Raspberry ◽  
Red Raspberry ◽  
Plant Vigor ◽  
First Report ◽  
Tobacco Ringspot Virus ◽  
The Past ◽  
Raspberry Bushy Dwarf Virus

Over the past 10 years, several commercial producers of black raspberry in Ohio have experienced a decline in plant vigor and survival in their plantings. In 2004, a survey of several black and red raspberry plantings in Ohio was conducted to determine if viruses were present. Of the 115 samples taken in the survey, Raspberry bushy dwarf (RBDV), Tomato ringspot (ToRSV), and Tobacco ringspot virus (TRSV) were detected in 21, 5, and 2, respectively. This is the first report of RBDV in Ohio. Accepted for publication 27 April 2005. Published 10 May 2005.

Molecular characterisation of Raspberry bushy dwarf virus isolates from Sweden and Belarus

2011 ◽  
Vol 156 (3) ◽  
pp. 369-374 ◽  
Author(s):  
N. Valasevich ◽  
N. Kukharchyk ◽  
A. Kvarnheden
Keyword(s):  
Dwarf Virus ◽  
Molecular Characterisation ◽  
Raspberry Bushy Dwarf Virus ◽  
Virus Isolates

scholarly journals First report of black raspberry necrosis virus and raspberry leaf mottle virus in Bosnia and Herzegovina

2021 ◽  
Author(s):  
Mariana Radulović ◽  
Irena Mavrič-Pleško ◽  
Francois Maclot ◽  
Duska Delić ◽  
Sebastien Massart
Keyword(s):  
Bosnia And Herzegovina ◽  
Mottle Virus ◽  
Black Raspberry ◽  
Necrosis Virus ◽  
First Report

Black raspberry necrosis virus. [Distribution map].

2010 ◽  
Author(s):  
Keyword(s):  
British Columbia ◽  
New Zealand ◽  
North America ◽  
Geographical Distribution ◽  
Black Raspberry ◽  
Distribution Map ◽  
Necrosis Virus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Black raspberry necrosis virus. Hosts: Rubus spp. Information is given on the geographical distribution in Europe (France, Germany, Italy, Mainland Italy, Netherlands, Norway, Poland, Romania, Serbia, UK, Scotland), North America (Canada, British Columbia, USA, Michigan, Oregon, Pennsylvania, Tennessee, Wisconsin), Oceania (Australia, New Zealand).

scholarly journals First Report of Raspberry bushy dwarf virus Infecting Grapevine in Hungary

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1582-1582 ◽  
Author(s):  
I. Mavrič Pleško ◽  
M. Viršček Marn ◽  
K. Nyerges ◽  
J. Lázár
Keyword(s):  
Natural Infection ◽  
Dwarf Virus ◽  
Molecular Characteristics ◽  
Vitis Vinifera L ◽  
Southwestern Part ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Two Samples ◽  
Rubus Species ◽  
Das Elisa

Raspberry bushy dwarf virus (RBDV) is the sole member of genus Idaeovirus and naturally infects Rubus species worldwide. It can be experimentally transmitted to many dicotyledonous plant species from different families. In Slovenia it has been reported to naturally infect grapevine, the first known non-Rubus natural host (3). However, RBDV from red raspberry and grapevine were found to be different in biological, serological, and molecular characteristics (4). From 2007 to 2010, grapevine (Vitis vinifera L.) vineyards were sampled in different parts of Hungary and tested for RBDV infection by double antibody sandwich (DAS)-ELISA using commercial reagents (Bioreba, Reinach, Switzerland). Overall, 181 samples were collected from 10 vineyards around Csörnyeföld, Badacsony, Eger, Tolcsva (Orémus), and Nagyréde. Samples were taken randomly unless plants showing virus-like symptoms were present, which were preferentially included in the survey. Two samples collected in 2010, each consisting of five leaves from five individual plants, tested positive by DAS-ELISA. They originated from a small private vineyard of Italian Riesling, Pinot Gris, and Rhein Riesling in the southwestern part of Hungary near Csörnyeföld where 29 samples were collected. All leaves were asymptomatic. Total RNA was extracted from positive samples using a RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized using primer RNA12 as described (4) and further amplified by PCR using primers RBDVUP1/RBDVLO4 that amplified an 872-bp fragment of RBDV coat protein and 3′ non-translated region (2). Amplification products from both samples were directly sequenced (Macrogen, Seoul, Korea). The sequences showed 98.6% identity between each other and were deposited in GenBank (Accession Nos. JQ928628 and JQ928629). Sequences were also compared with RBDV sequences deposited in GenBank. They showed 97.7 to 99.3% identity with RBDV sequences from grapevine from Slovenia and 94.2 to 96.1% with RBDV sequences from Rubus sp. Natural infection of grapevine with RBDV was first reported from Slovenia in 2003 (3) and was recently reported also from Serbia (1). To our knowledge, this is the first report of RBDV infection of grapevine in Hungary and suggests a wider presence of the virus in the region. References: (1) D. Jevremovic and S. Paunovic. Pestic. Phytomed. (Belgrade) 26:55, 2011. (2) H. I. Kokko et al. BioTechniques 20:842, 1996. (3) I. Mavric Pleško et al. Plant Dis. 87:1148, 2003. (4) I. Mavric Pleško et al. Eur. J. Plant Pathol. 123:261, 2009.

Raspberry bushy dwarf virus. [Distribution map].

2013 ◽  
Author(s):  
Keyword(s):  
New York ◽  
South Carolina ◽  
Central America ◽  
New Hampshire ◽  
Dwarf Virus ◽  
Distribution Map ◽  
Raspberry Bushy Dwarf Virus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Raspberry bushy dwarf virus. Idaeovirus. Main hosts: Rubus spp. Information is given on the geographical distribution in Europe (Belarus, Czech Republic, Finland, France, Germany, Hungary, Italy, Latvia, Lithuania, Poland, Romania, Serbia, Slovenia, Sweden, UK, England and Wales, Scotland), Asia (China, Guizhou, Japan, Honshu), Africa (South Africa), North America (Canada, British Columbia, USA, Alabama, Arkansas, California, Georgia, New Hampshire, New York, Ohio, Oregon, Pennsylvania, South Carolina, Washington), Central America & Caribbean (Costa Rica), South America (Chile, Ecuador), Oceania (Australia, Tasmania, New Zealand).

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