Virus‐like particles displaying recombinant Der p 1 zymogen to optimize IgG blocking antibody response

Allergy ◽  
2021 ◽  
Author(s):  
Sirikarn Jitthamstaporn ◽  
Adam F. Sander ◽  
Alain Jacquet
Keyword(s):  
Antibody Response ◽  
Blocking Antibody ◽  
Virus Like Particles ◽  
Der P 1

Der p 1 peptide on virus-like particles is safe and highly immunogenic in healthy adults

2006 ◽  
Vol 117 (6) ◽  
pp. 1470-1476 ◽  
Author(s):  
T KUNDIG ◽  
G SENTI ◽  
G SCHNETZLER ◽  
C WOLF ◽  
B PRINZVAVRICKA ◽  
...  
Keyword(s):  
Healthy Adults ◽  
Virus Like Particles ◽  
Der P 1

scholarly journals A Lentiviral Vector Expressing Japanese Encephalitis Virus-like Particles Elicits Broad Neutralizing Antibody Response in Pigs

2015 ◽  
Vol 9 (10) ◽  
pp. e0004081 ◽  
Author(s):  
Mélissanne de Wispelaere ◽  
Meret Ricklin ◽  
Philippe Souque ◽  
Marie-Pascale Frenkiel ◽  
Sylvie Paulous ◽  
...  
Keyword(s):  
Antibody Response ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Lentiviral Vector ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Virus Like Particles

scholarly journals Simultaneous Immunization with Multivalent Norovirus VLPs Induces Better Protective Immune Responses to Norovirus than Sequential Immunization

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1018
Author(s):  
Maria Malm ◽  
Timo Vesikari ◽  
Vesna Blazevic
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Antibody Response ◽  
Vaccine Candidate ◽  
Serum Antibodies ◽  
Blocking Antibody ◽  
Alternative Approach ◽  
Sequential Boost ◽  
Trivalent Vaccine ◽  
Rotavirus Vp6

Human noroviruses (NoVs) are a genetically diverse, constantly evolving group of viruses. Here, we studied the effect of NoV pre-existing immunity on the success of NoV vaccinations with genetically close and distant genotypes. A sequential immunization as an alternative approach to multivalent NoV virus-like particles (VLPs) vaccine was investigated. Mice were immunized with NoV GI.3, GII.4-1999, GII.17, and GII.4 Sydney as monovalent VLPs or as a single tetravalent mixture combined with rotavirus VP6-protein. Sequentially immunized mice were primed with a trivalent vaccine candidate (GI.3 + GII.4-1999 + VP6) and boosted, first with GII.17 and then with GII.4 Sydney VLPs. NoV serum antibodies were analyzed. Similar NoV genotype-specific immune responses were induced with the monovalent and multivalent mixture immunizations, and no immunological interference was observed. Multivalent immunization with simultaneous mix was found to be superior to sequential immunization, as sequential boost induced strong blocking antibody response against the distant genotype (GII.17), but not against GII.4 Sydney, closely related to GII.4-1999, contained in the priming vaccine. Genetically close antigens may interfere with the immune response generation and thereby immune responses may be differently formed depending on the degree of NoV VLP genotype identity.

scholarly journals Nasal Immunization of Mice with Human Papillomavirus Type 16 Virus-Like Particles Elicits Neutralizing Antibodies in Mucosal Secretions

1998 ◽  
Vol 72 (10) ◽  
pp. 8220-8229 ◽  
Author(s):  
Carole Balmelli ◽  
Richard Roden ◽  
Alexandra Potts ◽  
John Schiller ◽  
Pierre De Grandi ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Antibody Response ◽  
Neutralizing Antibodies ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Like Particles ◽  
Human Papillomavirus Type 16 ◽  
Specific Igg ◽  
Nasal Immunization ◽  
Mucosal Secretions

ABSTRACT To specifically induce a mucosal antibody response to purified human papillomavirus type 16 (HPV16) virus-like particles (VLP), we immunized female BALB/c mice orally, intranasally, and/or parenterally and evaluated cholera toxin (CT) as a mucosal adjuvant. Anti-HPV16 VLP immunoglobulin G (IgG) and IgA titers in serum, saliva, and genital secretions were measured by enzyme-linked immunosorbent assay (ELISA). Systemic immunizations alone induced HPV16 VLP-specific IgG in serum and, to a lesser extent, in genital secretions but no secretory IgA. Oral immunization, even in the presence of CT, was inefficient. However, three nasal immunizations with 5 μg of VLP given at weekly intervals to anesthetized mice induced high (>104) and long-lasting (>15 weeks) titers of anti-HPV16 VLP antibodies in all samples, including IgA and IgG in saliva and genital secretions. CT enhanced the VLP-specific antibody response 10-fold in serum and to a lesser extent in saliva and genital secretions. Nasal immunization of conscious mice compared to anesthetized mice was inefficient and correlated with the absence of uptake of a marker into the lung. However, a 1-μg VLP systemic priming followed by two 5-μg VLP intranasal boosts in conscious mice induced both HPV16 VLP-specific IgG and IgA in secretions, although the titers were lower than in anesthetized mice given three intranasal immunizations. Antibodies in serum, saliva, and genital secretions of immunized mice were strongly neutralizing in vitro (50% neutralization with ELISA titers of 65 to 125). The mucosal and systemic/mucosal HPV16 VLP immunization protocols that induced significant titers of neutralizing IgG and secretory IgA in mucosal secretions in mice may be relevant to genital HPV VLP-based human vaccine trials.

scholarly journals Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response

2001 ◽  
Vol 75 (22) ◽  
pp. 11010-11016 ◽  
Author(s):  
Catherine Fromantin ◽  
Béatrice Jamot ◽  
Jean Cohen ◽  
Lionel Piroth ◽  
Pierre Pothier ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lymph Node ◽  
Antibody Response ◽  
Cholera Toxin ◽  
Lymphoid Tissues ◽  
Intranasal Immunization ◽  
Virus Like Particles ◽  
Heat Labile ◽  
Lymph Node Cells

ABSTRACT We investigated the rotavirus-specific lymphocyte responses induced by intranasal immunization of adult BALB/c mice with rotavirus 2/6 virus-like particles (2/6-VLPs) of the bovine RF strain, by assessing the profile of cytokines produced after in vitro restimulation and serum and fecal antibody responses. The cytokines produced by splenic cells were first evaluated. Intranasal immunization with 50 μg of 2/6-VLPs induced a high serum antibody response, including immunoglobulin G1 (IgG1) and IgG2a, a weak fecal antibody response, and a mixed Th1/Th2-like profile of cytokines characterized by gamma interferon and interleukin 10 (IL-10) production and very low levels of IL-2, IL-4, and IL-5. Intranasal immunization with 10 μg of 2/6-VLPs coadministered with the mucosal adjuvants cholera toxin andEscherichia coli heat-labile toxin (LT) considerably enhanced the Th1/Th2-like response; notably, significant levels of IL-2, IL-4, and IL-5 were observed. Since rotavirus is an enteric pathogen, we next investigated the production of IL-2 and IL-5, as being representative of Th1 and Th2 responses, by Peyer's patch and mesenteric lymph node cells from mice immunized intranasally with 2/6-VLPs and LT. The results were compared to those obtained from splenic and cervical lymph node cells. We found that both cytokines were produced by cells from each of these lymphoid tissues. These results confirm the Th1/Th2-like response observed at the systemic level and show, on the assumption that T cells are the primary cells producing the cytokines after in vitro restimulation, that rotavirus-specific T lymphocytes are present in the intestine after intranasal immunization with 2/6-VLPs and LT.

scholarly journals Induction of a high-titered antibody response using HIV gag-EV71 VP1-based virus-like particles with the capacity to protect newborn mice challenged with a lethal dose of enterovirus 71

2018 ◽  
Vol 163 (7) ◽  
pp. 1851-1861 ◽  
Author(s):  
Xi Wang ◽  
Ke Dong ◽  
Min Long ◽  
Fang Lin ◽  
Zhaowei Gao ◽  
...  
Keyword(s):  
Antibody Response ◽  
Lethal Dose ◽  
Enterovirus 71 ◽  
Newborn Mice ◽  
Virus Like Particles

scholarly journals Low-Dose Immunization with Adenovirus Expressing the Thyroid-Stimulating Hormone Receptor A-Subunit Deviates the Antibody Response toward That of Autoantibodies in Human Graves’ Disease

Endocrinology ◽  
2004 ◽  
Vol 145 (1) ◽  
pp. 228-233 ◽  
Author(s):  
Chun-Rong Chen ◽  
Pavel Pichurin ◽  
Gregorio D. Chazenbalk ◽  
Holly Aliesky ◽  
Yuji Nagayama ◽  
...  
Keyword(s):  
Antibody Response ◽  
Low Dose ◽  
Standard Dose ◽  
Thyroid Stimulating Hormone ◽  
Similar Proportion ◽  
Graves Disease ◽  
Antibody Activity ◽  
Blocking Antibody ◽  
A Subunit ◽  
Viral Dose

Abstract Immunization with adenovirus expressing the TSH receptor (TSHR) induces hyperthyroidism in 25–50% of mice. Even more effective is immunization with a TSHR A-subunit adenovirus (65–84% hyperthyroidism). Nevertheless, TSHR antibody characteristics in these mice do not mimic accurately those of autoantibodies in typical Graves’ patients, with a marked TSH-blocking antibody response. We hypothesized that this suboptimal antibody response was consequent to the standard dose of TSHR-adenovirus providing too great an immune stimulus. To test this hypothesis, we compared BALB/c mice immunized with the usual number (1011) and with far fewer viral particles (109 and 107). Regardless of viral dose, hyperthyroidism developed in a similar proportion (68–80%) of mice. We then examined the qualitative nature of TSHR antibodies in each group. Sera from all mice had TSH binding-inhibitory (TBI) activity after the second immunization, with TBI values in proportion to the viral dose. After the third injection, all groups had near-maximal TBI values. Remarkably, in confirmation of our hypothesis, immunization with progressively lower viral doses generated TSHR antibodies approaching the characteristics of autoantibodies in human Graves’ disease as follows: 1) lower TSHR antibody titers on ELISA and 2) lower TSH-blocking antibody activity without decrease in thyroid-stimulating antibody activity. In summary, low-dose immunization with adenovirus expressing the free TSHR A-subunit provides an induced animal model with a high prevalence of hyperthyroidism as well as TSHR antibodies more closely resembling autoantibodies in Graves’ disease.

Chimeric Bacteriophage fr Virus-Like Particles Harboring the Immunodominant C-Terminal Region of Hamster Polyomavirus VP1 Induce a Strong VP1-Specific Antibody Response in Rabbits and Mice

2002 ◽  
Vol 15 (4) ◽  
pp. 627-643 ◽  
Author(s):  
Tatyana Voronkova ◽  
Adrian Grosch ◽  
Andris Kazaks ◽  
Velta Ose ◽  
Dace Skrastina ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Terminal Region ◽  
Specific Antibody Response ◽  
Virus Like Particles ◽  
Hamster Polyomavirus

scholarly journals Construction and Immunological Evaluation of Multivalent Hepatitis B Virus (HBV) Core Virus-Like Particles Carrying HBV and HCV Epitopes

2010 ◽  
Vol 17 (6) ◽  
pp. 1027-1033 ◽  
Author(s):  
Irina Sominskaya ◽  
Dace Skrastina ◽  
Andris Dislers ◽  
Denis Vasiljev ◽  
Marija Mihailova ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Antibody Response ◽  
T Cell Activation ◽  
Cell Activation ◽  
Cytotoxic T Lymphocyte ◽  
High Titer ◽  
Virus Like Particles ◽  
Chimeric Vlps ◽  
B Virus

ABSTRACT A multivalent vaccine candidate against hepatitis B virus (HBV) and hepatitis C virus (HCV) infections was constructed on the basis of HBV core (HBc) virus-like particles (VLPs) as carriers. Chimeric VLPs that carried a virus-neutralizing HBV pre-S1 epitope corresponding to amino acids (aa) 20 to 47 in the major immunodominant region (MIR) and a highly conserved N-terminal HCV core epitope corresponding to aa 1 to 60 at the C terminus of the truncated HBcΔ protein (N-terminal aa 1 to 144 of full-length HBc) were produced in Escherichia coli cells and examined for their antigenicity and immunogenicity. The presence of two different foreign epitopes within the HBc molecule did not interfere with its VLP-forming ability, with the HBV pre-S1 epitope exposed on the surface and the HCV core epitope buried within the VLPs. After immunization of BALB/c mice, specific T-cell activation by both foreign epitopes and a high-titer antibody response against the pre-S1 epitope were found, whereas an antibody response against the HBc carrier was notably suppressed. Both inserted epitopes also induced a specific cytotoxic-T-lymphocyte (CTL) response, as shown by the gamma interferon (IFN-γ) production profile.

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