Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

2019 ◽  
Vol 43 (8) ◽  
pp. 764-772 ◽  
Author(s):  
Carlos Eduardo B. Moura ◽  
Moacir F. Queiroz Neto ◽  
Janine Karla F. S. Braz ◽  
Michelle de Medeiros Aires ◽  
Naisandra B. Silva Farias ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Nitrided Titanium

scholarly journals Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Carlos Nelson Elias ◽  
Patricia Abdo Gravina ◽  
Costa e Silva Filho ◽  
Pedro Augusto de Paula Nascente
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Osteoblastic Cells ◽  
Acid Etching ◽  
Implant Surface ◽  
Force Microscopy ◽  
Dental Implant Surface ◽  
Before And After ◽  
Titanium Surfaces

Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength.Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed.Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells.Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of thein vitrocytocompatibility of the surfaces.Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants.

Osteogenesis of Rat Mesenchymal Stem Cells and Osteoblastic Cells on Strontium-Doped Nanohydroxyapatite-Coated Titanium Surfaces

2015 ◽  
Vol 30 (2) ◽  
pp. 461-471 ◽  
Author(s):  
Qiao-Hong Jiang ◽  
Xue Gong ◽  
Xiao-Xiang Wang ◽  
Fu-Ming He
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteoblastic Cells ◽  
Titanium Surfaces

Transcriptional profile of osteoblastic cells cultured on titanium surfaces modified by oxidative nanopatterning

2014 ◽  
Author(s):  
Maidy Ferreira ◽  
Fabio Variola ◽  
Antonio Nanci ◽  
Janaina Dernowsek ◽  
Geraldo Passos ◽  
...  
Keyword(s):  
Transcriptional Profile ◽  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Cells Cultured

The effect of plasma-nitrided titanium surfaces on osteoblastic cell adhesion, proliferation, and differentiation

2013 ◽  
Vol 102 (4) ◽  
pp. 991-998 ◽  
Author(s):  
Emanuela P. Ferraz ◽  
Juliana C. Sa ◽  
Paulo T. de Oliveira ◽  
Clodomiro Alves ◽  
Marcio M. Beloti ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Osteoblastic Cell ◽  
Proliferation And Differentiation ◽  
Titanium Surfaces ◽  
Nitrided Titanium

Physical and chemical effects of the hydrogen irradiation on nitrided titanium surfaces

2017 ◽  
Vol 312 ◽  
pp. 91-100 ◽  
Author(s):  
Gelson B. de Souza ◽  
Silvio H. Gonsalves ◽  
Karen C. Ribeiro ◽  
Dair G. Ditzel ◽  
Mario Ueda ◽  
...  
Keyword(s):  
Chemical Effects ◽  
Titanium Surfaces ◽  
Physical And Chemical ◽  
Nitrided Titanium

scholarly journals Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces

2018 ◽  
Vol 52 (1) ◽  
pp. 12-18
Author(s):  
Sinem Yeniyol ◽  
John Lawrance Ricci
Keyword(s):  
Alkaline Phosphatase ◽  
Osteoblastic Cells ◽  
Titanium Surfaces

scholarly journals ALP LEVELS OF MURINE PRE-OSTEOBLASTIC CELLS ON ANODIZED AND ANNEALED TITANIUM SURFACES

2017 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Yeniyol ◽  
John Lawrence Ricci
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces

scholarly journals Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Maidy Rehder Wimmers Ferreira ◽  
Roger Rodrigo Fernandes ◽  
Amanda Freire Assis ◽  
Janaína A. Dernowsek ◽  
Geraldo A. Passos ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Control Cell ◽  
Titanium Implants ◽  
Osteoblastic Cells ◽  
Surface Features ◽  
Cellular Events ◽  
Tissue Interactions ◽  
Biochemical Assays ◽  
Titanium Surfaces ◽  
Dental Applications

Titanium implants have been extensively used in orthopedic and dental applications. It is well known that micro- and nanoscale surface features of biomaterials affect cellular events that control implant-host tissue interactions. To improve our understanding of how multiscale surface features affect cell behavior, we used microarrays to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cultured on engineered titanium surfaces, exhibiting the following topographies: nanotexture (N), nano+submicrotexture (NS), and rough microtexture (MR), obtained by modulating experimental parameters (temperature and solution composition) of a simple yet efficient chemical treatment with a H2SO4/H2O2solution. Biochemical assays showed that cell culture proliferation augmented after 10 days, and cell viability increased gradually over 14 days. Among the treated surfaces, we observed an increase of alkaline phosphatase activity as a function of the surface texture, with higher activity shown by cells adhering onto nanotextured surfaces. Nevertheless, the rough microtexture group showed higher amounts of calcium than nanotextured group. Microarray data showed differential expression of 716 mRNAs and 32 microRNAs with functions associated with osteogenesis. Results suggest that oxidative nanopatterning of titanium surfaces induces changes in the metabolism of osteoblastic cells and contribute to the explanation of the mechanisms that control cell responses to micro- and nanoengineered surfaces.

Adhesion pattern and growth of primary human osteoblastic cells on five commercially available titanium surfaces

2010 ◽  
Vol 21 (7) ◽  
pp. 756-765 ◽  
Author(s):  
Giovanni Passeri ◽  
Antonio Cacchioli ◽  
Francesca Ravanetti ◽  
Carlo Galli ◽  
Erida Elezi ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Human Osteoblastic Cells ◽  
Adhesion Pattern

In vitro assays for adhesion and migration of osteoblastic cells (Saos-2) on titanium surfaces

2006 ◽  
Vol 324 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Chun-yu Li ◽  
Shuang-yan Gao ◽  
Takehiro Terashita ◽  
Tetsuya Shimokawa ◽  
Haruyuki Kawahara ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
In Vitro Assays ◽  
Titanium Surfaces ◽  
And Migration
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