scholarly journals Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces

2018 ◽  
Vol 52 (1) ◽  
pp. 12-18
Author(s):  
Sinem Yeniyol ◽  
John Lawrance Ricci
Keyword(s):  
Alkaline Phosphatase ◽  
Osteoblastic Cells ◽  
Titanium Surfaces

scholarly journals Necessity of Enzymatic Activity of Alkaline Phosphatase for Mineralization of Osteoblastic Cells

2002 ◽  
Vol 88 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Yuki Sugawara ◽  
Kuniaki Suzuki ◽  
Mino Koshikawa ◽  
Masaki Ando ◽  
Junichiro Iida
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Osteoblastic Cells

The influence of titanium surfaces treated by alkalis on macrophage and osteoblast-like cell adhesion and gene expression in vitro

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81378-81387 ◽  
Author(s):  
Ting Ma ◽  
Xi-Yuan Ge ◽  
Sheng-Nan Jia ◽  
Xi Jiang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Cell Adhesion ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Related Gene ◽  
Titanium Surfaces

The effect of alkali-treated titanium surfaces on inflammation-related gene expression of macrophages and alkaline phosphatase activity of osteoblast-like cells.

Expression of parathyroid hormone-related peptide (PTHrP) and PTH/PTHrP receptor in newborn human calvaria osteoblastic cells

1997 ◽  
Vol 136 (6) ◽  
pp. 640-648 ◽  
Author(s):  
Abderrahim Lomri ◽  
Cindy de Pollak ◽  
Michael Sebag ◽  
David Goltzman ◽  
Richard Kremer ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Culture Media ◽  
Receptor Expression ◽  
Osteoblastic Cells ◽  
Pcr Analysis ◽  
Intracellular Camp ◽  
Related Peptide ◽  
Parathyroid Hormone Related Peptide ◽  
Pthrp Receptor

Abstract We examined the expression of parathyroid hormone-related peptide (PTHrP) and its receptor in normal newborn human calvaria osteoblastic (NHCO) cells. Northern blot analysis showed that NHCO cells express a single 1·6 kb transcript of PTHrP, which was increased within 1 h (2x) and peaked at 6 h (7x) after serum treatment. In the culture media, the release of PTHrP peptide was maximally increased (4x) 24 h after the addition of serum, as determined by immunoradiometric assay. NHCO cells exhibited a cytoplasmic immunostaining for PTHrP in the presence of serum, and most PTHrP-positive cells were alkaline phosphatase-negative, suggesting that PTHrP was expressed in undifferentiated cells. Furthermore, RT-PCR analysis showed that both PTHrP and PTH/PTHrP receptor were expressed in NHCO cells in basal conditions or after stimulation with serum. The maximal PTHrP expression induced by serum suppressed PTH/PTHrP receptor expression, suggesting that PTHrP down-regulated its receptor in NHCO cells. Treatment with 10 nm human PTH(1–34—which binds to PTH/PTHrP receptors, increased intracellular cAMP levels and alkaline phosphatase activity, and decreased cell growth, indicating that ligand binding to PTH/PTHrP receptors regulates NHCO cell proliferation and differentiation. The expression and synthesis of PTHrP and the presence of functional PTH/PTHrP receptors suggest a possible paracrine mechanism of action of PTHrP in normal human calvaria osteoblastic cells. European Journal of Endocrinology 136 640–648

scholarly journals Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

2007 ◽  
Vol 12 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Maria Helena Santos ◽  
Ana Paula M. Shaimberg ◽  
Patricia Valerio ◽  
Alfredo M. Goes ◽  
Maria de Fátima Leite ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Orthophosphoric Acid ◽  
Type I Collagen ◽  
Enzymatic Digestion ◽  
Osteoblastic Cells ◽  
Type I ◽  
Post Test ◽  
Synthetic Hydroxyapatite

The cytocompatibility of synthetic hydroxyapatite/collagen composites alone or doped with Zn+2 was tested by using primary culture of osteoblasts. The hydroxyapatite (HAP) was synthesized having calcium hydroxide and orthophosphoric acid as precursors. A new HAP composite was developed adding 1.05 w% of Zn(NO3)2.6H2O forming HAPZn. The pure type I collagen (COL) was obtained from bovine pericardium by enzymatic digestion method. The HAP/COL and HAPZn/COL composites were developed and characterized by SEM/EDS. The cell viability and alkaline phosphatase activity in the presence of composites were evaluated by MTT assay and NBT-BCIP assay, respectively, and compared to osteoblastic cells of the control. Three individual experiments were accomplished in triplicates and submitted to the variance analysis and Bonferroni’s post-test with statistically significant at p<0.05. The HAPZn/COL composite did not stimulate the proliferation and increasing of alkaline phosphatase activity of the osteoblastic cells. The tested composites did not alter the cellular viability neither caused alterations in the cellular morphology in 72 h showing adequate properties for biological applications.

Inductive Effects of Prostaglandins on Alkaline Phosphatase in Osteoblastic Cells, Clone MC3T3-E11

1985 ◽  
Vol 97 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Yoshiyuki HAKEDA ◽  
Yoshinori NAKATANI ◽  
Masahiko HIRAMATSU ◽  
Noriyoshi KURIHARA ◽  
Mayuri TSUNOl ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Osteoblastic Cells ◽  
Inductive Effects

scholarly journals Effect of glucose concentration in media on alkaline phosphatase activity and mineralization in osteoblastic cells.

1996 ◽  
Vol 71 ◽  
pp. 121
Author(s):  
Yoshitaka Yoshimura ◽  
Yoshiaki Deyama ◽  
Shin-ichi Tanaka ◽  
Kuniaki Suzuki ◽  
Akira Matsumoto
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Glucose Concentration ◽  
Osteoblastic Cells ◽  
Effect Of Glucose

Low-dose parathyroid hormone and estrogen reverse alkaline phosphatase activity suppressed by dexamethasone in mouse osteoblastic cells

2005 ◽  
Vol 23 (6) ◽  
pp. 450-455 ◽  
Author(s):  
Mei-Fway Iu ◽  
Hiroshi Kaji ◽  
Junko Naito ◽  
Hideaki Sowa ◽  
Toshitsugu Sugimoto ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Low Dose ◽  
Osteoblastic Cells
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