Impact of heat shock protein 60KD in combination with outer membrane proteins on immune response againstBrucella melitensis

Apmis ◽  
2017 ◽  
Vol 126 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Tooba Abbassi-Daloii ◽  
Soheil Yousefi ◽  
Mohammad Hadi Sekhavati ◽  
Mojtaba Tahmoorespur
Keyword(s):  
Immune Response ◽  
Heat Shock ◽  
Membrane Proteins ◽  
Heat Shock Protein ◽  
Outer Membrane ◽  
Outer Membrane Proteins

scholarly journals Small Heat Shock Protein Hsp17.8 Functions as an AKR2A Cofactor in the Targeting of Chloroplast Outer Membrane Proteins in Arabidopsis

2011 ◽  
Vol 157 (1) ◽  
pp. 132-146 ◽  
Author(s):  
Dae Heon Kim ◽  
Zheng-Yi Xu ◽  
Yun Jeong Na ◽  
Yun-Joo Yoo ◽  
Junho Lee ◽  
...  
Keyword(s):  
Heat Shock ◽  
Membrane Proteins ◽  
Heat Shock Protein ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Small Heat Shock Protein

scholarly journals Protein and antigen profiles of Leptospira interrogans serovar Hardjo

2009 ◽  
Vol 39 (9) ◽  
pp. 2539-2543
Author(s):  
Bárbara Nobre Lafetá ◽  
Elaine Cristina de Castro ◽  
Nivaldo da Silva
Keyword(s):  
Immune Response ◽  
Membrane Proteins ◽  
Western Blot ◽  
Outer Membrane ◽  
Protein Profile ◽  
Outer Membrane Proteins ◽  
Rabbit Antiserum ◽  
Leptospira Interrogans ◽  
Sds Page ◽  
Hyperimmune Rabbit

The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.

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