Comparative transcriptome sequencing of the hepatopancreas reveals differentially expressed genes in the precocious juvenile Chinese mitten crab, Eriocheir sinensis (Crustacea: Decapoda)

2016 ◽  
Vol 48 (7) ◽  
pp. 3645-3656 ◽  
Author(s):  
Yu Xu ◽  
Xuguang Li ◽  
Yanfei Deng ◽  
Quanping Lu ◽  
Yuanjie Yang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Transcriptome Sequencing ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Comparative Transcriptome ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongbo Jiang ◽  
Jie Bao ◽  
Yuenan Xing ◽  
Chengcheng Feng ◽  
Xiaodong Li ◽  
...  
Keyword(s):  
Serine Protease ◽  
Light Chain ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Related Factor ◽  
Differentially Expressed Proteins ◽  
Chinese Mitten Crab ◽  
Monitoring Method ◽  
Elastase Inhibitor ◽  
Mitten Crab

The “milky disease” of the Chinese mitten crab, Eriocheir sinensis, is a highly lethal fungal disease caused by Metschnikowia bicuspidata infection. To elucidate the immune responses of the hemolymph of E. sinensis to M. bicuspidata infection, a comparative analysis of the hemolymph of E. sinensis infected with M. bicuspidata and that treated with phosphate buffered saline was performed using label-free quantitative proteomics. A total of 429 proteins were identified. Using a 1.5-fold change in expression as a physiologically significant benchmark, 62 differentially expressed proteins were identified, of which 38 were significantly upregulated and 24 were significantly downregulated. The upregulated proteins mainly included cytoskeleton-related proteins (myosin regulatory light chain 2, myosin light chain alkali, tubulin α-2 chain, and tubulin β-1 chain), serine protease and serine protease inhibitor (clip domain-containing serine protease, leukocyte elastase inhibitor, serine protein inhibitor 42Dd), catalase, transferrin, and heat shock protein 70. Upregulation of these proteins indicated that phenoloxidase system, phagocytosis and the ROS systems were induced by M. bicuspidata. The downregulated proteins were mainly organ and tissue regeneration proteins (PDGF/VEGF-related factor protein, integrin-linked protein kinase homing pat-4 gene) and hemagglutination-associated proteins (hemolymph clottable protein, hemocyte protein-glutamine gamma-glutamyltransferase). Downregulation of these proteins indicated that M. bicuspidata inhibited hemocyte regeneration and hemolymph agglutination. Fifteen differentially expressed proteins related to immunity were verified using a parallel reaction monitoring method. The expression trend of these proteins was similar to that of the proteome. To the best of our knowledge, this is the first report on the proteome of E. sinensis in response to M. bicuspidata infection. These results not only provide new and important information on the immune response of crustaceans to yeast infection but also provide a basis for further understanding the molecular mechanism of complex host pathogen interactions between crustaceans and fungi.

scholarly journals The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210414 ◽  
Author(s):  
Yang-yang Pang ◽  
Cong Zhang ◽  
Min-jie Xu ◽  
Gen-yong Huang ◽  
Yong-xu Cheng ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcriptome Sequencing ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

Transcriptome sequencing revealed the genes and pathways involved in salinity stress of Chinese mitten crab, Eriocheir sinensis

2014 ◽  
Vol 46 (5) ◽  
pp. 177-190 ◽  
Author(s):  
Erchao Li ◽  
Shaolin Wang ◽  
Chao Li ◽  
Xiaodan Wang ◽  
Ke Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Salinity Stress ◽  
Transcriptome Sequencing ◽  
Muscle Protein ◽  
Expression Patterns ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Control Group ◽  
Protein Ubiquitination ◽  
Mitten Crab

A total of 276.9 million reads were obtained and assembled into 206, 371 contigs with an average length of 614 bp and N50 of 1,470 bp. Comparison of digital gene expression between treatment and control group reveals 1,151 and 941 genes were significantly differentially expressed in crab gill and muscle, respectively. In gill and muscle, protein ubiquitination, ubiquinone biosynthesis, oxidative phosphorylation, and mitochondria dysfunction pathways were the top pathways differentially expressed following the challenge. EIF 2 signaling pathway and IGF-1 signaling pathway were the top ones among the signal-related pathways. Most of the amino acid metabolism pathways were found to be involved in this process. The expression patterns of 15 differentially expressed genes were validated by quantitative real-time RT-PCR (average correlation coefficient 0.80). This is the first report of expression analysis of genes and pathways involved in osmoregulation of Eriocheir sinensis through transcriptome sequencing. The findings of this study will further promote the understanding of the underlying molecular mechanism of salinity stress adaptation for crustacean species.

Comparative transcriptome sequencing of the intestine reveals differentially expressed genes in Pelteobagrus vachellii

2018 ◽  
Vol 49 (7) ◽  
pp. 2560-2571 ◽  
Author(s):  
Chuanjie Qin ◽  
Jiaxian Sun ◽  
Zhengyong Wen ◽  
Yongwang Han ◽  
Yongfu Liu ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Transcriptome Sequencing ◽  
Differentially Expressed ◽  
Comparative Transcriptome

scholarly journals The Innate Immune Response to Infection by Polyascus gregaria in the Male Chinese Mitten Crab (Eriocheir sinensis), Revealed by Proteomic Analysis

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 57
Author(s):  
Yanping Yang ◽  
Fengjiao Ma ◽  
Juanjuan Dong ◽  
Lianxing Li ◽  
Ping Ren ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Protein Level ◽  
Eriocheir Sinensis ◽  
Innate Immune ◽  
Differentially Expressed ◽  
Economic Losses ◽  
Chinese Mitten Crab ◽  
Kegg Pathways ◽  
Mitten Crab

The Chinese mitten crab (Eriocheir sinensis) is a representative catadromous invertebrate of the Yangtze River and a commercial species widely cultivated in China. Both cultivated and wild crabs suffer from a variety of parasites and pathogens, which can result in catastrophic economic losses in aquaculture revenue. Polyascus gregaria, a parasitic barnacle with a highly derived morphology, is specialized in invading these crabs. This study examines the immunological mechanism in E. sinensis infected with P. gregaria. Tandem mass tags (TMT), a specialized method of mass-spectrometry, was used to analyze the infection by P. gregaria resistance at the protein level. In the hepatopancreas of infected crabs, 598 proteins differentially expressed relating to physiological change, of which, 352 were upregulated and 246 were downregulated. Based on this differential protein expression, 104 GO terms and 13 KEGG pathways were significantly enriched. Differentially expressed proteins, such as ATG, cathepsin, serpin, iron-related protein, Rab family, integrin, and lectin, are associated with the lysosome GO term and the autophagy-animal KEGG pathways, both of which likely relate to the immune response to the parasitic P. gregaria infection. These results show the benefit of taking a detailed, protein-level approach to understanding the innate immune response of aquatic invertebrates to macroparasite infection.

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