Possible involvement of PKC/MAPK pathway in the regulation of GnRH by dietary arachidonic acid in the brain of male tongue sole Cynoglossus semilaevis

2019 ◽  
Vol 50 (12) ◽  
pp. 3528-3538 ◽  
Author(s):  
Houguo Xu ◽  
Bo Sun ◽  
Zhangbin Liao ◽  
Elena Pribytkova ◽  
Qinggong Zhang ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Mapk Pathway ◽  
Cynoglossus Semilaevis ◽  
Dietary Arachidonic Acid ◽  
Tongue Sole ◽  
The Brain

Dietary arachidonic acid differentially regulates the gonadal steroidogenesis in the marine teleost, tongue sole ( Cynoglossus semilaevis ), depending on fish gender and maturation stage

Aquaculture ◽  
2017 ◽  
Vol 468 ◽  
pp. 378-385 ◽  
Author(s):  
Houguo Xu ◽  
Lin Cao ◽  
Yuanqin Zhang ◽  
Ronald B. Johnson ◽  
Yuliang Wei ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Cynoglossus Semilaevis ◽  
Maturation Stage ◽  
Marine Teleost ◽  
Dietary Arachidonic Acid ◽  
Tongue Sole

scholarly journals Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis

2021 ◽  
Vol 12 ◽  
Author(s):  
Yani Dong ◽  
Likang Lyu ◽  
Haishen Wen ◽  
Bao Shi
Keyword(s):  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Interaction ◽  
Cynoglossus Semilaevis ◽  
Rna Seq ◽  
Tongue Sole ◽  
Gene Interaction Networks ◽  
Half Smooth Tongue Sole ◽  
The Brain

Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.

scholarly journals The effect of dietary arachidonic acid (ARA) on growth performance, fatty acid composition and expression of ARA metabolism-related genes in larval half-smooth tongue sole (Cynoglossus semilaevis)

2015 ◽  
Vol 113 (10) ◽  
pp. 1518-1530 ◽  
Author(s):  
Yuhui Yuan ◽  
Songlin Li ◽  
Kangsen Mai ◽  
Wei Xu ◽  
Yanjiao Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arachidonic Acid ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Growth Performance ◽  
Acid Composition ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole ◽  
Cox 2 ◽  
Half Smooth Tongue Sole

The present study was conducted to investigate the effects of dietary arachidonic acid (ARA) on growth performance, fatty acid composition and ARA metabolism-related gene expression in larval half-smooth tongue sole (Cynoglossus semilaevis). Larvae (35 d after hatching, 54 (sem 1) mg) were fed diets with graded concentrations of ARA (0·01, 0·39, 0·70, 1·07, 1·42 and 2·86 % dry weight) five times per d to apparent satiation for 30 d. Results showed that increased dietary ARA concentration caused a significant non-linear rise to a plateau in survival rate, final body weight and thermal growth coefficient, and the maximum values occurred with the 1·42 % ARA treatment. As dietary ARA increased to 1·07 or 1·42 %, activities of trypsin, leucine aminopeptidase and alkaline phosphatase levels increased, but they decreased with higher ARA concentrations. The fatty acid composition of tongue sole larvae was almost well correlated with their dietary fatty acid profiles, and the EPA content of the larvae decreased with increasing dietary ARA. Meanwhile, the partial sequences of COX-1a (cyclo-oxygenase-1a), COX-1b (cyclo-oxygenase-1b), COX-2 (cyclo-oxygenase-2), 5-LOX (5-lipoxygenase) and CYP2J6-like (cytochrome P450 2J6-like) were also obtained. Both COX-2 and 5-LOX mRNA expression levels significantly increased to a plateau in an ‘L’-shaped manner as dietary ARA increased to 1·07 or 1·42 %, but no significant differences were found in the gene expression of COX-1a, COX-1b or CYP2J6-like. These results suggest that 1·07–1·42 % dietary ARA was beneficial to the growth performance of larval tongue sole, and the regulation of dietary ARA on the growth performance of larvae was probably involved in altering the mRNA expression of COX-2 and 5-LOX.

Isolation and characterization of microsatellite markers for half- smooth tongue sole (Cynoglossus semilaevis) and an analysis of seg-regation in the F1

2013 ◽  
Vol 18 (6) ◽  
pp. 1384-1391
Author(s):  
Liming JIANG ◽  
Haiyang YU ◽  
Quanqi ZHANG ◽  
Zhipeng LIU ◽  
Junwei LIU ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole ◽  
Isolation And Characterization ◽  
Half Smooth Tongue Sole

Cloning and expression of anti-Müllerian hormone gene in half- smooth tongue-sole, Cynoglossus semilaevis

2013 ◽  
Vol 20 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Shanshan LIU ◽  
Bing SUN ◽  
Zhuo LIANG ◽  
Jing ZHANG ◽  
Songlin CHEN
Keyword(s):  
Cynoglossus Semilaevis ◽  
Cloning And Expression ◽  
Tongue Sole ◽  
Half Smooth Tongue Sole

Skeletal deformities in the larvae and juveniles of cultured tongue sole (Cynoglossus semilaevis)

2013 ◽  
Vol 18 (6) ◽  
pp. 1399-1405
Author(s):  
Hui MA ◽  
Zhimeng ZHUANG ◽  
Shufang LIU ◽  
Qian MA ◽  
Xiuli WANG
Keyword(s):  
Cynoglossus Semilaevis ◽  
Skeletal Deformities ◽  
Larvae And Juveniles ◽  
Tongue Sole
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