Effects of two exogenous proteins on the insulin‐like androgenic gland hormone gene expression in Procambarus clarkii

2021 ◽  
Author(s):  
Jin Yan ◽  
Bingyan Zheng ◽  
Kianann Tan ◽  
Shaokui Yi ◽  
Yanhe Li
Keyword(s):  
Gene Expression ◽  
Procambarus Clarkii ◽  
Androgenic Gland

5-Hydroxytryptaminergic control of testes development through the androgenic gland in the red swamp crayfish,Procambarus clarkii

1994 ◽  
Vol 26 (2) ◽  
pp. 127-132 ◽  
Author(s):  
RACHAKONDA SAROJINI ◽  
RACHAKONDA NAGABHUSHANAM ◽  
MILTON FINGERMAN
Keyword(s):  
Procambarus Clarkii ◽  
Androgenic Gland ◽  
Red Swamp Crayfish

scholarly journals Differential effects of silencing crustacean hyperglycemic hormone gene expression on the metabolic profiles of the muscle and hepatopancreas in the crayfish Procambarus clarkii

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172557 ◽  
Author(s):  
Wenfeng Li ◽  
Kuo-Hsun Chiu ◽  
Yi-Chun Tien ◽  
Shih-Fu Tsai ◽  
Li-Jane Shih ◽  
...  
Keyword(s):  
Gene Expression ◽  
Procambarus Clarkii ◽  
Metabolic Profiles ◽  
Crustacean Hyperglycemic Hormone ◽  
Differential Effects ◽  
Hyperglycemic Hormone

scholarly journals Regulation of amino acid and nucleotide metabolism by crustacean hyperglycemic hormone in the muscle and hepatopancreas of the crayfish Procambarus clarkii

2019 ◽  
Author(s):  
Wenfeng Li ◽  
Kuo-Hsun Chiu ◽  
Chi-Ying Lee
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Fatty Acid Biosynthesis ◽  
Procambarus Clarkii ◽  
Nucleotide Metabolism ◽  
Metabolic Effects ◽  
Crustacean Hyperglycemic Hormone ◽  
Pyruvate Metabolism ◽  
Metabolic Pathway Analysis ◽  
Hyperglycemic Hormone

AbstractTo comprehensively characterize the metabolic roles of crustacean hyperglycemic hormone (CHH), metabolites in two CHH target tissues of the crayfish Procambarus clarkii, whose levels were significantly different between CHH-silenced and saline-treated control animals, were analyzed using bioinformatics tools provided by an on-line analysis suite (MetaboAnalyst). Analysis with Metabolic Pathway Analysis (MetPA) indicated that in the muscle Glyoxylate and dicarboxylate metabolism, Nicotinate and nicotinamide metabolism, Alanine, aspartate and glutamate metabolism, Pyruvate metabolism, and Nitrogen metabolism were significantly affected by silencing of CHH gene expression at 24 hours post injection (hpi), while only Nicotinate and nicotinamide metabolism remained significantly affected at 48 hpi. In the hepatopancreas, silencing of CHH gene expression significantly impacted, at 24 hpi, Pyruvate metabolism and Glycolysis or gluconeogenesis, and at 48 hpi, Glycine, serine and threonine metabolism. Moreover, analysis using Metabolite Set Enrichment Analysis (MSEA) showed that many metabolite sets were significantly affected in the muscle at 24hpi, including Ammonia recycling, Nicotinate and nicotinamide metabolism, Pyruvate metabolism, Purine metabolism, Warburg effect, Citric acid cycle, and metabolism of several amino acids, and at 48 hpi only Nicotinate and nicotinamide metabolism, Glycine and serine metabolism, and Ammonia recycling remained significantly affected. In the hepatopancreas, MSEA analysis showed that Fatty acid biosynthesis was significantly impacted at 24 hpi. Finally, in the muscle, levels of several amino acids decreased significantly, while those of 5 other amino acids or related compounds significantly increased in response to CHH gene silencing. Levels of metabolites related to nucleotide metabolism significantly decreased across the board at both time points. In the hepatopancreas, the effects were comparatively minor with only levels of thymine and urea being significantly decreased at 24 hpi. The combined results showed that the metabolic effects of silencing CHH gene expression were far more diverse than suggested by previous studies that emphasized on carbohydrate and energy metabolism. Based on the results, metabolic roles of CHH on the muscle and hepatopancreas were summarized and discussed.

Photopigment gene expression and rhabdom formation in the crayfish ( Procambarus clarkii )

2003 ◽  
Vol 311 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Hafner G. ◽  
Martin R. ◽  
Tokarski T.
Keyword(s):  
Gene Expression ◽  
Procambarus Clarkii

Silicone stressed response of crayfish (Procambarus clarkii) in antioxidant enzyme activity and related gene expression

2020 ◽  
pp. 115836
Author(s):  
Md Muzammel Hossain ◽  
Hengliang Huang ◽  
Yuan Yuan ◽  
Tianyin Wan ◽  
Chengfeng Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Procambarus Clarkii ◽  
Antioxidant Enzyme Activity ◽  
Related Gene

Citrobacter freundii infection in red swamp crayfish (Procambarus clarkii) and host immune-related gene expression profiles

Aquaculture ◽  
2020 ◽  
Vol 515 ◽  
pp. 734499 ◽  
Author(s):  
Xiaodan Liu ◽  
Xiao He ◽  
Zhenhua An ◽  
Wei Sun ◽  
Nan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Procambarus Clarkii ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Citrobacter Freundii ◽  
Related Gene ◽  
Immune Related Gene ◽  
Red Swamp Crayfish

Androgenic Gland and Secondary Sexual Characters in the Crayfish Procambarus Clarkii

1990 ◽  
Vol 10 (3) ◽  
pp. 492-497 ◽  
Author(s):  
Yoko Taketomi ◽  
Masahiro Murata ◽  
Mitsuharu Miyawaki
Keyword(s):  
Procambarus Clarkii ◽  
Androgenic Gland ◽  
Secondary Sexual Characters ◽  
Secondary Sexual

scholarly journals Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 645 ◽  
Author(s):  
Linlin Shi ◽  
Shuxin Han ◽  
Jiamin Fei ◽  
Long Zhang ◽  
Jonathan W Ray ◽  
...  
Keyword(s):  
Procambarus Clarkii ◽  
Functional Study ◽  
Distribution Analysis ◽  
Androgenic Gland ◽  
Secondary Sexual Characteristics ◽  
Single Sex ◽  
Red Swamp Crayfish ◽  
Rnai Technology ◽  
Sexual Characteristics ◽  
Male Specific

The androgenic gland (AG) is a male-specific endocrine organ that controls the primary and secondary sexual characteristics in male crustaceans. More evidence indicates that the insulin-like androgenic gland hormone gene (IAG) is the key male sexual differentiation factor, particularly the application of RNA interference (RNAi) technology on IAG. In this study, the full-length cDNA of IAG (termed PcIAG) was isolated from the red swamp crayfish, Procambarus clarkii. Tissue distribution analysis showed that in addition to its expression in the AG of male P. clarkii, PcIAG was widely expressed in female tissues and other male tissues. The PcIAG protein was detected in the reproductive and nervous systems of adult male P. clarkii. Additionally, RNAi results showed that the PcIAG expression could be silenced efficiently, and the male sperm maturation and release possibly present a transient adverse interference at lower doses (0.1 μg/g and 1 μg/g) of PcIAG–dsRNA (PcIAG double-stranded RNA). Dramatically, the expression level of PcIAG increased sharply shortly after the injection of higher doses (5 μg/g and 10 μg/g) of PcIAG–dsRNA, which might accelerate the maturation and release of sperm. Moreover, the expression of PcSxl (P. clarkii Sex-lethal) was detected by Quantitative Real-Time PCR (qPCR) after the injection of PcIAG–dsRNA to explore whether the PcIAG gene regulates the PcSxl gene, and we found that the PcIAG did not directly regulate PcSxl in P. clarkii. The study could help accelerate the progress of PcIAG functional research and provide a useful reference for the single-sex selective breeding of P. clarkii.

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