Hoxa11 and Hoxa13 facilitate slow‐twitch muscle formation in C2C12 cells and indirectly affect the lipid deposition of 3T3‐L1 cells

2021 ◽  
Vol 92 (1) ◽  
Author(s):  
Xiangsheng Lin ◽  
Mengting Yan ◽  
Wensai Yu ◽  
Yuchen Ma ◽  
Lifan Zhang ◽  
...  
Keyword(s):  
C2c12 Cells ◽  
Lipid Deposition ◽  
Muscle Formation ◽  
Slow Twitch Muscle ◽  
Slow Twitch

scholarly journals Mdfi Promotes C2C12 Cell Differentiation and Positively Modulates Fast-to-Slow-Twitch Muscle Fiber Transformation

2021 ◽  
Vol 9 ◽  
Author(s):  
Bo Huang ◽  
Yiren Jiao ◽  
Yifan Zhu ◽  
Zuocheng Ning ◽  
Zijian Ye ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Muscle Fiber ◽  
Fiber Type ◽  
C2c12 Cell ◽  
C2c12 Cells ◽  
Muscle Fiber Type ◽  
Rna Seq ◽  
Type Transformation ◽  
Slow Twitch Muscle ◽  
Slow Twitch

Muscle development requires myoblast differentiation and muscle fiber formation. Myod family inhibitor (Mdfi) inhibits myogenic regulatory factors in NIH3T3 cells, but how Mdfi regulates myoblast myogenic development is still unclear. In the present study, we constructed an Mdfi-overexpression (Mdfi-OE) C2C12 cell line by the CRISPR/Cas9 system and performed RNA-seq on Mdfi-OE and wild-type (WT) C2C12 cells. The RNA-seq results showed that the calcium signaling pathway was the most significant. We also established the regulatory networks of Mdfi-OE on C2C12 cell differentiation and muscle fiber type transformation and identified hub genes. Further, both RNA-seq and experimental verification demonstrated that Mdfi promoted C2C12 cell differentiation by upregulating the expression of Myod, Myog, and Myosin. We also found that the positive regulation of Mdfi on fast-to-slow-twitch muscle fiber transformation is mediated by Myod, Camk2b, and its downstream genes, such as Pgc1a, Pdk4, Cs, Cox4, Acadm, Acox1, Cycs, and Atp5a1. In conclusion, our results demonstrated that Mdfi promotes C2C12 cell differentiation and positively modulates fast-to-slow-twitch muscle fiber transformation. These findings further our understanding of the regulatory mechanisms of Mdfi in myogenic development and muscle fiber type transformation. Our results suggest potential therapeutic targets for muscle- and metabolic-related diseases.

Lycopene increases the proportion of slow-twitch muscle fiber by AMPK signaling to improve muscle anti-fatigue ability

2021 ◽  
pp. 108750
Author(s):  
Wanxue Wen ◽  
Xiaoling Chen ◽  
Zhiqing Huang ◽  
Daiwen Chen ◽  
Bing Yu ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Ampk Signaling ◽  
Slow Twitch Muscle ◽  
Slow Twitch

scholarly journals lncRNA-Six1 Is a Target of miR-1611 that Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 243 ◽  
Author(s):  
Manting Ma ◽  
Bolin Cai ◽  
Liang Jiang ◽  
Bahareldin Ali Abdalla ◽  
Zhenhui Li ◽  
...  
Keyword(s):  
Fiber Type ◽  
Muscle Fibers ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Proliferation And Differentiation ◽  
Slow Twitch Muscle ◽  
Fast Twitch Muscle ◽  
Slow Twitch ◽  
Slow Twitch Fibers ◽  
Fast Twitch

Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate Six1 in cis to participate in myogenesis. Here, we studied a microRNA (miRNA) that is specifically expressed in chickens (miR-1611). Interestingly, miR-1611 was found to contain potential binding sites for both lncRNA-Six1 and Six1, and it can interact with lncRNA-Six1 to regulate Six1 expression. Overexpression of miR-1611 represses the proliferation and differentiation of myoblasts. Moreover, miR-1611 is highly expressed in slow-twitch fibers, and it drives the transformation of fast-twitch muscle fibers to slow-twitch muscle fibers. Together, these data demonstrate that miR-1611 can mediate the regulation of Six1 by lncRNA-Six1, thereby affecting proliferation and differentiation of myoblasts and transformation of muscle fiber types.

ATP depletion in slow-twitch red muscle of rat

1987 ◽  
Vol 253 (3) ◽  
pp. C426-C432 ◽  
Author(s):  
D. M. Whitlock ◽  
R. L. Terjung
Keyword(s):  
Adenine Nucleotide ◽  
Atp Depletion ◽  
Cellular Events ◽  
Contraction Condition ◽  
Red Muscle ◽  
Slow Twitch Muscle ◽  
Fast Twitch Muscle ◽  
Slow Twitch ◽  
Fast Twitch

Rat slow-twitch muscle, in contrast to fast-twitch muscle, maintains its ATP content near normal during intense stimulation conditions that produce rapid fatigue. An extensive depletion of adenine nucleotide content by the deamination of AMP to IMP + NH3, typical of fast-twitch muscle, does not occur. We evaluated whether this response of slow-twitch muscle could be simply due to failure of synaptic transmission or related to cellular conditions influencing enzyme activity. Stimulation of soleus muscles in situ via the nerve or directly in the presence of curare at 120 tetani/min for 3 min resulted in extensive fatigue but normal ATP contents. Thus the lack of ATP depletion must be related to cellular events distal to neuromuscular transmission. Even nerve and direct muscle stimulation (with curare) during ischemia did not cause a large depletion of ATP or a large elevation of lactate content (12.0 +/- 0.7 mumol/g), even though the decline in tension was essentially complete. However, if the same tension decline during ischemia was prolonged by stimulating for 10 min at 12 tetani/min a large decrease in ATP (2.24 +/- 0.09 mumol/g) and increase in IMP (2.47 +/- 0.16 mumol/g) and lactate (30.4 +/- 2.0 mumol/g) content occurred. Thus adenine nucleotide deamination to IMP can occur in slow-twitch muscle during specific contraction conditions. The cellular events leading to the activation of AMP deaminase require an intense contraction condition and may be related to acidosis caused by a high lactate content.

Involvement of fast and slow twitch muscle fibres in avian muscular dystrophy

1983 ◽  
Vol 61 (2) ◽  
pp. 217-233 ◽  
Author(s):  
John A. Pizzey ◽  
Eric A. Barnard ◽  
Penelope J. Barnard
Keyword(s):  
Muscular Dystrophy ◽  
Muscle Fibres ◽  
Slow Twitch Muscle ◽  
Slow Twitch

scholarly journals Analysis of the rabbit cardiac/slow twitch muscle sarcoplasmic reticulum calcium ATPase (SERCA2) gene promoter

1993 ◽  
Vol 21 (11) ◽  
pp. 2723-2728 ◽  
Author(s):  
Drew A. Sukovich ◽  
Junaid Shabbeer ◽  
Muthu Periasamy
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Gene Promoter ◽  
Calcium Atpase ◽  
Slow Twitch Muscle ◽  
Slow Twitch ◽  
Sarcoplasmic Reticulum Calcium
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