Metabolic interactions of benzodiazepines with oxycodone ex vivo and toxicity depending on usage patterns in an animal model

Diabetes mellitus (DM) is a multisystem endocrine disorder affecting the brain. Mesenchymal stem cells (MSCs) pretreated with Melatonin have been shown to increase the potency of MSCs. This work aimed to compare Melatonin, stem cells, and stem cells pretreated with Melatonin on the cognitive functions and markers of synaptic plasticity in an animal model of type I diabetes mellitus (TIDM). Thirty-six rats represented the animal model; six rats for isolation of MSCs and 30 rats were divided into five groups: control, TIDM, TIDM + Melatonin, TIDM + Stem cells, and TIDM + Stem ex vivo Melatonin. Functional assessment was performed with Y-maze, forced swimming test and novel object recognition. Histological and biochemical evaluation of hippocampal Neuroligin 1, Sortilin, Brain-Derived Neurotrophic Factor (BDNF), inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2), Tumor necrosis factor-alpha (TNF-α), and Growth Associated Protein 43 (GAP43). The TIDM group showed a significant decrease of hippocampal Neuroligin, Sortilin, and BDNF and a significant increase in iNOS, TNF-α, TLR2, and GAP43. Melatonin or stem cells groups showed improvement compared to the diabetic group but not compared to the control group. TIDM + Stem ex vivo Melatonin group showed a significant improvement, and some values were restored to normal. Ex vivo melatonin-treated stem cells had improved spatial working and object recognition memory and depression, with positive effects on glucose homeostasis, inflammatory markers levels and synaptic plasticity markers expression.

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Cervical spinal fusion enabled by a ceramic implant in an animal model; ex vivo CT micromotion analysis

Brain and Spine ◽

10.1016/j.bas.2021.100168 ◽

2021 ◽

Vol 1 ◽

pp. 100168

Author(s):

Maria Östman ◽

Peter Försth ◽

Håkan Engqvist ◽

Michael Pujari-Palmer ◽

Franck Forterre ◽

...

Keyword(s):

Animal Model ◽

Spinal Fusion ◽

Ex Vivo ◽

Ceramic Implant

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Antithrombotic effect of a monoclonal antibody to the platelet glycoprotein IIb/IIIa receptor in an experimental animal model

Blood ◽

10.1182/blood.v68.3.783.bloodjournal683783 ◽

1986 ◽

Vol 68 (3) ◽

pp. 783-786 ◽

Cited By ~ 1

Author(s):

BS Coller ◽

JD Folts ◽

LE Scudder ◽

SR Smith

Keyword(s):

Monoclonal Antibody ◽

Animal Model ◽

Platelet Aggregation ◽

Ex Vivo ◽

Thrombus Formation ◽

Platelet Glycoprotein ◽

Antithrombotic Effect ◽

Antithrombotic Agent ◽

Antithrombotic Effects ◽

Platelet Thrombus

A murine monoclonal antibody directed at the platelet glycoprotein IIb/IIIa complex, which blocks platelet aggregation ex vivo, was tested for its antithrombotic effects in an established animal model of acute platelet thrombus formation in partially stenosed arteries. Infusion of 0.7 to 0.8 mg/kg of the F(ab')2 fragment of the antibody completely blocked new thrombus formation despite multiple provocations, making it the most potent antithrombotic agent tested in this model.

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Cross-Species Pharmacologic Evaluation of Plasmin as a Direct-Acting Thrombolytic Agent: Ex Vivo Evaluation for Large Animal Model Development

Journal of Vascular and Interventional Radiology ◽

10.1097/01.rvi.0000148828.40438.d3 ◽

2005 ◽

Vol 16 (3) ◽

pp. 369-377 ◽

Cited By ~ 10

Author(s):

Kyle Landskroner ◽

Neil Olson ◽

Gary Jesmok

Keyword(s):

Animal Model ◽

Thrombolytic Agent ◽

Ex Vivo ◽

Model Development ◽

Large Animal Model ◽

Large Animal ◽

Direct Acting

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V.01.2 PER-ORAL ENDOSCOPIC MYOTOMY (POEM) WITH A NEW THERAPEUTIC LASER SYSTEM: FIRST STUDY IN AN EX VIVO ANIMAL MODEL (WITH VIDEO)

Digestive and Liver Disease ◽

10.1016/s1590-8658(16)30123-2 ◽

2016 ◽

Vol 48 ◽

pp. e119

Author(s):

G.E. Tontini ◽

H. Neumann ◽

L. Carmignani ◽

B. Bruni ◽

P. Soriani ◽

...

Keyword(s):

Animal Model ◽

Ex Vivo ◽

Laser System ◽

Per Oral Endoscopic Myotomy ◽

Endoscopic Myotomy ◽

Per Oral

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Pantoprazole reduces vascular relaxation in-vitro and ex-vivo and interferes with blood coagulation in an animal model

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.05.084 ◽

2018 ◽

Vol 104 ◽

pp. 537-541

Author(s):

Azher M. Arafah ◽

Ajaz Ahmad ◽

Basit L. Jan ◽

Khalid M. Maghawi ◽

Mohammed A. Alharbi ◽

...

Keyword(s):

Animal Model ◽

Blood Coagulation ◽

Ex Vivo ◽

Vascular Relaxation

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Demonstration of endothelial adhesion of sickle cells in vivo: a distinct role for deformable sickle cell discocytes

Blood ◽

10.1182/blood.v79.6.1602.1602 ◽

1992 ◽

Vol 79 (6) ◽

pp. 1602-1611 ◽

Cited By ~ 37

Author(s):

ME Fabry ◽

E Fine ◽

V Rajanayagam ◽

SM Factor ◽

J Gore ◽

...

Keyword(s):

Animal Model ◽

Intact Animal ◽

Ex Vivo ◽

Sickle Cells ◽

Endothelial Monolayers ◽

Quantitative Studies ◽

Tissue Edema ◽

In Vivo Animal Model ◽

Von Willebrand

Abstract Different morphologic and density classes of sickle cells (SS) may play distinct roles in the generation of vasoocclusion, explaining the complexity of this phenomena. The densest SS red blood cells (RBCs) (SS4) can induce vasoocculsion in ex vivo microcirculatory preparations as well as in an intact animal model. Previous studies of the interaction of SS deformable discocytes with endothelial monolayers or the rat ex vivo mesocecum preparation have shown adhesion that is desmopressin (dDAVP)-stimulated, von Willebrand factor (vWF)-mediated, and limited to the small venules. However, in vivo adhesion of SS RBCs to the endothelium has neither been demonstrated nor characterized; and, in particular, the relation of adhesion to vasoocclusion is unknown. Using an intact animal model that involves injecting saline- washed, density-defined SS RBCs into the femoral artery of a rat, we find that: (1) Quantitative studies of RBCs retained in the rat thigh using 99mTc-labeled RBCs and gamma camera imaging showed that dDAVP induces a threefold increase in retention of normal (AA) cells and deformable SS discocytes (SS2). (2) electron microscopy and Microfil injection show that the retention of SS2 cells is due to adhesion to the vascular endothelium with no evidence of obstruction. (3) H-1 magnetic resonance imaging showed that retention of SS4 cells induced a dose-dependent increase in tissue edema (presumable secondary to tissue hypoxia), while retention of AA or SS2 cells produced no change. We conclude that endothelial adhesion of deformable SS discocytes can be demonstrated in an in vivo animal model, that this adhesion is enhanced by dDAVP (presumably related to, but not necessarily limited to the release of vWF), and that this phenomenon per se does not lead to vasoocclusion. Nevertheless, adhesion of deformable SS discocytes may have consequences. We hypothesize that adhesion of SS discocytes could narrow the lumen of postcapillary venules and facilitate secondary trapping of SS4 cells and lead to subsequent vasoocclusion.

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