scholarly journals EAO‐438/PO‐BR‐010 | Streptococcus oralis biofilm formation on Nd:YAG laser‐textured zirconia implant surfaces

2021 ◽  
Vol 32 (S22) ◽  
pp. 75-75
Keyword(s):  
Nd:Yag Laser ◽  
Biofilm Formation ◽  
Streptococcus Oralis ◽  
Zirconia Implant

scholarly journals Identification of the Binding Domain of Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase for Porphyromonas gingivalis Major Fimbriae

2009 ◽  
Vol 77 (11) ◽  
pp. 5130-5138 ◽  
Author(s):  
Hideki Nagata ◽  
Mio Iwasaki ◽  
Kazuhiko Maeda ◽  
Masae Kuboniwa ◽  
Ei Hashino ◽  
...  
Keyword(s):  
Amino Acid ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Binding Activity ◽  
Binding Domain ◽  
Laser Scanning Microscopy ◽  
Dependent Manner ◽  
Oral Streptococci ◽  
Amino Acid Residues ◽  
Streptococcus Oralis

ABSTRACT Porphyromonas gingivalis forms communities with antecedent oral biofilm constituent streptococci. P. gingivalis major fimbriae bind to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) present on the streptococcal surface, and this interaction plays an important role in P. gingivalis colonization. This study identified the binding domain of Streptococcus oralis GAPDH for P. gingivalis fimbriae. S. oralis recombinant GAPDH (rGAPDH) was digested with lysyl endopeptidase. Cleaved fragments of rGAPDH were applied to a reverse-phase high-pressure liquid chromatograph equipped with a C18 column. Each peak was collected; the binding activity toward P. gingivalis recombinant fimbrillin (rFimA) was analyzed with a biomolecular interaction analysis system. The fragment displaying the strongest binding activity was further digested with various proteinases, after which the binding activity of each fragment was measured. The amino acid sequence of each fragment was determined by direct sequencing, mass spectrometric analysis, and amino acid analysis. Amino acid residues 166 to 183 of S. oralis GAPDH exhibited the strongest binding activity toward rFimA; confocal laser scanning microscopy revealed that the synthetic peptide corresponding to amino acid residues 166 to 183 of S. oralis GAPDH (pep166-183, DNFGVVEGLMTTIHAYTG) inhibits S. oralis-P. gingivalis biofilm formation in a dose-dependent manner. Moreover, pep166-183 inhibited interbacterial biofilm formation by several oral streptococci and P. gingivalis strains with different types of FimA. These results indicate that the binding domain of S. oralis GAPDH for P. gingivalis fimbriae exists within the region encompassing amino acid residues 166 to 183 of GAPDH and that pep166-183 may be a potent inhibitor of P. gingivalis colonization in the oral cavity.

scholarly journals Effects of Bleaching Associated with Er:YAG and Nd:YAG Laser on Enamel Structure and Bacterial Biofilm Formation

Scanning ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Xiuxiu Hou ◽  
Keyong Yuan ◽  
Zhengwei Huang ◽  
Rui Ma
Keyword(s):  
Nd:Yag Laser ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Fusobacterium Nucleatum ◽  
Bacterial Biofilm ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Streptococcus Sanguis ◽  
Enamel Structure ◽  
Significant Difference

Objective. To compare the effects of bleaching associated with Er:YAG and Nd:YAG laser on enamel structure and mixed biofilm formation on teeth surfaces. Materials and Methods. Sixty-eight enamel samples were randomly divided into four groups ( n = 17 ), control, Opalescence Boost only, Opalescence Boost plus Er: YAG laser, and Opalescence Boost plus Nd:YAG laser. The structure was observed using SEM after bleaching. Subsequently, the treated enamel samples were also cultured in suspensions of Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, and Fusobacterium nucleatum (Fn) for 24 and 48 h. Biofilm formation was quantified by crystal violet staining, and the structure was visualized by confocal laser scanning microscopy. The data were analyzed using the Kruskal-Wallis method. Results. The enamel structure significantly changed after bleaching. There was no obvious difference in the biofilm formation after 24 h; however, after 48 hours, the amount of biofilm increased significantly. Remarkably, the amount was significantly higher on enamel bleached only, however, there was no significant difference between samples bleached with Er:YAG or Nd:YAG laser compared to the control. Conclusions. Bleaching only appeared to markedly promote biofilm formation after 48 h, and the biofilms on samples bleached with Er:YAG or Nd:YAG laser did not change significantly, showing that bleaching with Er:YAG or Nd:YAG laser can be safely applied in clinical practice.

scholarly journals Understanding Lactobacillus paracasei and Streptococcus oralis Biofilm Interactions through Agent-Based Modeling

mSphere ◽  
2021 ◽  
Author(s):  
Linda Archambault ◽  
Sherli Koshy-Chenthittayil ◽  
Angela Thompson ◽  
Anna Dongari-Bagtzoglou ◽  
Reinhard Laubenbacher ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Oral Candidiasis ◽  
Agent Based Modeling ◽  
Cell Models ◽  
Complex Environments ◽  
Inhibitory Effects ◽  
Content Type ◽  
Agent Based ◽  
Streptococcus Oralis

We previously discovered a role of the oral commensal Streptococcus oralis as an accessory pathogen. S. oralis increases the virulence of Candida albicans infections in murine oral candidiasis and epithelial cell models through mechanisms which promote the formation of tissue-damaging biofilms. Lactobacillus species have known inhibitory effects on biofilm formation of many microbes, including Streptococcus species. Agent-based modeling has great advantages as a means of exploring multifaceted relationships between organisms in complex environments such as biofilms.

scholarly journals A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract Streptococcus oralis Biofilm Formation

2021 ◽  
Vol 11 (24) ◽  
pp. 11915
Author(s):  
Simonetta D’Ercole ◽  
Carlo Mangano ◽  
Luigina Cellini ◽  
Silvia Di Lodovico ◽  
Cigdem Atalayin Ozkaya ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Titanium Surface ◽  
Selective Laser Sintering ◽  
Human Saliva ◽  
Laser Sintering ◽  
Lower Percentage ◽  
Antibiofilm Activity ◽  
Streptococcus Oralis ◽  
3D Surface ◽  
Microbiological Assays

The topography of implant surfaces influences the interaction relationship between material and bacteria. The aim of this work was to characterize a novel 3D titanium surface, produced using Selective Laser Sintering (SLS), and to compare the bacterial interaction with machined and double acid etching (DAE) discs. The surface was characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDX). The wettability was measured using the sessile method. The microbiological investigation consisted in the cultivation of a bacterial pioneer, Streptococcus oralis, on titanium surfaces, previously covered by human saliva in order to form the acquired pellicle. Then, colony forming units (CFUs), biofilm biomass quantification, analyses of viable and dead cells, and SEM observation were determined after 24 h of S. oralis biofilm formation on the different discs. A significantly higher nano-roughness with respect to the other two groups characterized the novel 3D surface, but the wettability was similar to that of machined samples. The microbiological assays demonstrated that the 3D discs reported significantly lower values of CFUs and biofilm biomass with respect to machined surfaces; however, no significant differences were found with the DAE surfaces. The live/dead staining confirmed the lower percentage of living cells on DAE and 3D surfaces compared with the machined. This novel 3D surface produced by SLS presented a high antiadhesive and antibiofilm activity.

scholarly journals Osteoblasts behavior on macrotextured zirconia implant surfaces by Nd:YAG laser – in vitro study

2020 ◽  
Vol 31 (S20) ◽  
pp. 114-114
Author(s):  
Mariana Cruz ◽  
Beatriz Fernandes ◽  
Joana Marques ◽  
Sara Madeira ◽  
Oscar Carvalho ◽  
...  
Keyword(s):  
Nd:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Zirconia Implant

In Vitro Biofilm Formation on Titanium and Zirconia Implant Surfaces

2017 ◽  
Vol 88 (3) ◽  
pp. 298-307 ◽  
Author(s):  
Stefan Roehling ◽  
Monika Astasov-Frauenhoffer ◽  
Irmgard Hauser-Gerspach ◽  
Olivier Braissant ◽  
Henriette Woelfler ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Zirconia Implant

Evaluation of Streptococcus oralis adhesion and biofilm formation on laser-processed titanium

2021 ◽  
Vol 7 (2) ◽  
pp. 875-878
Author(s):  
Katharina Doll ◽  
Vadim Veiko ◽  
Yulia Karlagina ◽  
Galina Odintsova ◽  
Nils Heine ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Biofilm Formation ◽  
Antibacterial Properties ◽  
Pulsed Laser ◽  
Pulsed Laser Ablation ◽  
Surface Modifications ◽  
Bacterial Colonisation ◽  
Streptococcus Oralis ◽  
Nanosecond Pulsed Laser ◽  
Titanium Surfaces

Abstract To prevent implant-associated infections, surface modifications need to be developed that prevent bacterial colonisation and biofilm formation. In the present study, titanium surfaces were processed by nanosecond-pulsed laser ablation to generate a variety of different structures (anatase, rutile, Osteon, as well as Osteon additionally coated with silver and clove nanoparticles). Analysis of adhesion and biofilm formation of the oral pioneer bacterium Streptococcus oralis could demonstrate antibacterial properties of anatase surfaces. For clinical translation, the effect should be enhanced by further adaption and combined with the osseointegrative Osteon structure

Streptococcus oralis Biofilm Formation on Titanium Surfaces

2021 ◽  
Vol 36 (5) ◽  
pp. 929-936
Author(s):  
Simonetta D’Ercole ◽  
Emanuela Di Campli ◽  
Serena Pilato ◽  
Giovanna Iezzi ◽  
Luigina Cellini ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Streptococcus Oralis ◽  
Titanium Surfaces
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