scholarly journals Origin of langerin (CD207)‐expressing antigen presenting cells in the normal oral mucosa and in oral lichen planus lesions

2021 ◽  
Author(s):  
Maren B. Solhaug ◽  
Olav Schreurs ◽  
Karl Schenck ◽  
Inger Johanne Blix ◽  
Espen S. Baekkevold
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Antigen Presenting Cells ◽  
Normal Oral Mucosa ◽  
Antigen Presenting ◽  
Oral Lichen

scholarly journals Overexpression and Selectively Regulatory Roles of IL-23/IL-17 Axis in the Lesions of Oral Lichen Planus

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Rui Lu ◽  
Xin Zeng ◽  
Qi Han ◽  
Mu Lin ◽  
Long Long ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Chronic Inflammatory Disease ◽  
Immune Disorders ◽  
Oral Keratinocytes ◽  
Regulatory Pathway ◽  
Normal Oral Mucosa ◽  
Oral Lichen

Interleukin- (IL-) 23/IL-17 axis is a newly discovered proinflammatory signaling pathway and has been implicated in the pathogenesis of many chronic inflammatory and immune disorders. Here we investigated whether the IL-23/IL-17 axis was present and functional in the lesions of oral lichen planus (OLP), a chronic inflammatory disease affecting the oral mucosa. Using immunohistochemistry and quantitative PCR, we found that the subunits of IL-23 and IL-17 were overexpressed in OLP lesions than in normal oral mucosa tissues. In addition, the expressions of IL-23 and IL-17 are positively correlated in reticular OLP tissues. Results from in vitro studies revealed that exogenous IL-23 could increase the percentage of Th17 cells and IL-17 production in the CD4+T cells from reticular OLP patients. Furthermore, we also found that exogenous IL-17 could significantly enhance the mRNA expressions ofβ-defensin-2, -3, CCL-20, IL-8, and TNF-α, but notβ-defensin-1, CXCL-9, -10, -11, CCL-5, and IL-6 in human oral keratinocytes. Taken together, our results revealed an overexpression pattern and selectively regulatory roles of IL-23/IL-17 axis in the OLP lesions, suggesting that it may be a pivotal regulatory pathway in the complex immune network of OLP lesions.

scholarly journals Brief communication (Original). Trefoil factor expression by immunohistochemistry in patients with oral lichen planus

2014 ◽  
Vol 8 (6) ◽  
pp. 743-749 ◽  
Author(s):  
Ponlatham Chaiyarit ◽  
Poramaporn Klanrit ◽  
Pensri Phothipakdee ◽  
Ajiravudh Subarnbhesaj ◽  
Kobkan Thongprasom ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Mucosal Damage ◽  
Immunohistochemical Method ◽  
Tissue Samples ◽  
Normal Oral Mucosa ◽  
Increased Risk ◽  
Oral Lichen ◽  
Oral Epithelia

Abstract Background: Oral lichen planus (OLP) is a chronic immune-mediated inflammatory disease that usually causes oral mucosal damage. OLP has been considered to be a potentially malignant disorder associated with an increased risk for oral cancer. Trefoil factors (TFFs) are mainly synthesized by mucin-producing epithelial cells. Expression of TFFs in oral epithelia is thought to be an essential factor in protection against oral mucosal damage. However, very little is known about the connection between tissue damage of oral mucosa in OLP patients and expression of TFFs. Objectives: To determine levels of TFF expression in oral mucosa from patients with OLP. Methods: Tissue samples were collected from 35 patients with normal oral mucosa (controls) and 35 patients with OLP. An immunohistochemical method was used to determine the expression of the 3 TFFs (TFF1, 2, and 3) in mucosal tissues of the oral cavity. Results:TFF2 and TFF3 expression in oral epithelia were significantly decreased in OLP patients (Mann- Whitney U test; P = 0.006, and P = 0.002, respectively). There were no significant differences in TFF1 expression between OLP patients and control subjects. Conclusion: The present study demonstrated reduced expression of TFF2 and TFF3 in oral epithelia of OLP patients. These outcomes support our previous observations that chronic inflammation may play a major role in downregulation of TFF expression, which may be associated with oral mucosal damage in OLP patients.

scholarly journals Correlation of Mast Cell and Angiogenesis in Oral Lichen Planus, Dysplasia (Leukoplakia), and Oral Squamous Cell Carcinoma

2021 ◽  
Vol 12 (2) ◽  
pp. e0016
Author(s):  
Amutha Sundararajan ◽  
◽  
Rajmohan Muthusamy ◽  
Kumar Gopal Siva ◽  
Prasad Harikrishnan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cell ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Oral Lichen Planus ◽  
Normal Oral Mucosa ◽  
Oral Lichen

Objective: The aim of this study was to compare and correlate mast cell density (MCD) and microvessel density (MVD) between normal oral mucosa, oral lichen planus, various grades of dysplasia, and oral squamous cell carcinoma (OSCC). Materials and Methods: The study comprised a total of 75 samples, of which 65 were archival tissue blocks of histopathologically confirmed cases, which included 10 cases of oral lichen planus, 25 cases of dysplasia (mild [n=10], moderate [n=10], and severe [n=5]), and 30 cases of OSCC (well differentiated [n=10], moderately differentiated [n=10], and poorly differentiated [n=10]), and 10 samples of normal oral mucosa. All the sections were immunohistochemically stained with anti-CD34 and counterstained with toluidine blue stain. Mean MCD and MVD were determined and analyzed using ANOVA test and compared between the lesions using Tukey HSD test. Pearson’s correlation coefficient test was used to correlate these two factors between various lesions. Results: Mean MCD and mean MVD were found to be increased in all the lesions compared to normal oral mucosa, and the values were statically significant. Overall, MCD and MVD showed a significant positive correlation (r=0.640). Conclusion: Increase of MCD and MVD and their positive correlation in all the lesions have emphasized their role in the pathogenesis and disease progression.

Criteria for assessing severity of oral lichen planus

MedAlliance ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 73-81
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Severity Score ◽  
Oral Lichen Planus ◽  
Unknown Etiology ◽  
Altered Level ◽  
Doppler Flowmetry ◽  
Serum Free ◽  
Control Subjects ◽  
Oral Lichen

Introduction. Oral lichen planus (OLP) is an autoim- mune disease of unknown etiology, manifested in vari- ous clinical forms. A correlation between intensity of OLP local manifestations and severity of general disturbances predisposing to altered oral mucosa have been poorly investigated. The aim of our study was to identify criteria for assessing OLP severity score. Methods. OLP patients and control subjects (not OLP) matched by hepatobiliary disease and type IIa dyslipidemia were examined. Samples collected during the study were as- sessed by way clinical examination, gas chromatographic analysis of serum free fatty acids (FFA), oral fluid, serum and fecal microbial marker analysis by gas chromatogra- phy/mass spectrometry, laser Doppler flowmetry of the oral mucosa, immunohistochemistry staining with an- ti-CD68 monoclonal antibody specific to apolipoprotein B-100-bearing macrophages. Results. It was found that OLP patients significantly differed from control subjects in the three FFA levels (lauric acid, eicosopentaenoic/ lauric and eicosopentaenoic/linoleic ratios), displayed four pathologic factors matching the altered level of mi- crobial markers. In addition, OLP patients were found to differ in oral mucosa blood filling both inside and outside lesions paralleled with oxidative changes immediately in oral mucosa highlighted as elevated amount of apo- lipoprotein B-100-bearing CD68-positive macrophages. Conclusion. OLP severity score is an integrate parameter which reflects oxidative changes and alterations in oral mucosa microhemocirculation primarily in serum free fat- ty acid composition, altered oral and gut microbiota as well as intensity score of clinical picture.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

scholarly journals Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness

2008 ◽  
Vol 19 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Juliana M. Caldeira Brant ◽  
Anilton C. Vasconcelos ◽  
Luciana V. Rodrigues
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Chronic Inflammatory Disease ◽  
Mechanical Trauma ◽  
Epithelial Thickness ◽  
Healthy Mucosa ◽  
Toxic Agents ◽  
Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epitelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.

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