The impact of desmin on texture and water-holding capacity of ice-stored grass carp (Ctenopharyngodon idella) fillet

2016 ◽  
Vol 52 (2) ◽  
pp. 464-471 ◽  
Author(s):  
Jinhong Zang ◽  
Yanshun Xu ◽  
Wenshui Xia ◽  
Qixing Jiang
Keyword(s):  
Grass Carp ◽  
Water Holding Capacity ◽  
Ctenopharyngodon Idella ◽  
Grass Carp Ctenopharyngodon Idella ◽  
The Impact ◽  
Water Holding

The impact of collagen on softening of grass carp (Ctenopharyngodon idella) fillets stored under superchilled and ice storage

2015 ◽  
Vol 50 (11) ◽  
pp. 2427-2435 ◽  
Author(s):  
Xiaoqing Jiang ◽  
Yanshun Xu ◽  
Lihong Ge ◽  
Wenshui Xia ◽  
Qixing Jiang
Keyword(s):  
Grass Carp ◽  
Ctenopharyngodon Idella ◽  
Ice Storage ◽  
Grass Carp Ctenopharyngodon Idella ◽  
The Impact

scholarly journals Effect of Probiotics (Bacillus subtilis) on the Growth and Survival of Fingerlings of Grass Carp, Ctenopharyngodon idella

2021 ◽  
pp. 31-37
Author(s):  
Shahziya Shah ◽  
Anayitullah Chesti ◽  
Mansoor Rather ◽  
Mehak Hafeez ◽  
Anam Aijaz ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Grass Carp ◽  
Water Quality Management ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Efficiency Ratio ◽  
Grass Carp Ctenopharyngodon Idella ◽  
The Impact

In the modern high intensity aquaculture, probiotics offer an encouraging substitute to chemicals and antibiotics, one such important application of probiotics is their use as growth promoters, in addition to health and water quality management. On the same background, the study was carried to evaluate the effect of dietary incorporation of probiotic - Bacillus subtilis on the growth performance of grass carp (Ctenopharyngodon idella).The probiotic - Bacillus subtilis was mixed with the basal diet (Protein 32%) in three different concentrations (0.5, 1.0 and 1.5 % designated as T1, T2, and T3). The basal feed with no probiotic was used as control (T0). The impact was recorded for a period of 60 days. Feeding was done twice a day at the rate of 5% of their body weight. Growth performance was evaluated through estimation of weight gain, feed Conversion Ratio (FCR), protein Efficiency Ratio (PER), specific growth rate (SGR) and feed efficiency ratio (FER). It was observed that the probiotic Bacillussubtilis fed at 1.5% significantly improved the growth performance of the fish Ctenopharyngodon idella showing the highest growth rate followed by 1% and 0.5% probiotic fed diets. Lowest growth rate was recorded in control group.

scholarly journals Oral Vaccination of Grass Carp (Ctenopharyngodon idella) with Baculovirus-Expressed Grass Carp Reovirus (GCRV) Proteins Induces Protective Immunity against GCRV Infection

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Changyong Mu ◽  
Qiwang Zhong ◽  
Yan Meng ◽  
Yong Zhou ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Grass Carp ◽  
Oral Vaccine ◽  
Serum Antibody ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Oral Vaccination ◽  
Grass Carp Reovirus ◽  
Silkworm Pupae ◽  
Grass Carp Ctenopharyngodon Idella

The grass carp reovirus (GCRV) causes severe hemorrhagic disease with high mortality and leads to serious economic losses in the grass carp (Ctenopharyngodon idella) industry in China. Oral vaccine has been proven to be an effective method to provide protection against fish viruses. In this study, a recombinant baculovirus BmNPV-VP35-VP4 was generated to express VP35 and VP4 proteins from GCRV type Ⅱ via Bac-to-Bac baculovirus expression system. The expression of recombinant VP35-VP4 protein (rVP35-VP4) in Bombyx mori embryo cells (BmE) and silkworm pupae was confirmed by Western blotting and immunofluorescence assay (IFA) after infection with BmNPV-VP35-VP4. To vaccinate the grass carp by oral route, the silkworm pupae expressing the rVP35-VP4 proteins were converted into a powder after freeze-drying, added to artificial feed at 5% and fed to grass carp (18 ± 1.5 g) for six weeks, and the immune response and protective efficacy in grass carp after oral vaccination trial was thoroughly investigated. This included blood cell counting and classification, serum antibody titer detection, immune-related gene expression and the relative percent survival rate in immunized grass carp. The results of blood cell counts show that the number of white blood cells in the peripheral blood of immunized grass carp increased significantly from 14 to 28 days post-immunization (dpi). The differential leukocyte count of neutrophils and monocytes were significantly higher than those in the control group at 14 dpi. Additionally, the number of lymphocytes increased significantly and reached a peak at 28 dpi. The serum antibody levels were significantly increased at Day 14 and continued until 42 days post-vaccination. The mRNA expression levels of immune-related genes (IFN-1, TLR22, IL-1β, MHC I, Mx and IgM) were significantly upregulated in liver, spleen, kidney and hindgut after immunization. Four weeks post-immunization, fish were challenged with virulent GCRV by intraperitoneal injection. The results of this challenge study show that orally immunized group exhibited a survival rate of 60% and relative percent survival (RPS) of 56%, whereas the control group had a survival rate of 13% and RPS of 4%. Taken together, our results demonstrate that the silkworm pupae powder containing baculovirus-expressed VP35-VP4 proteins could induce both non-specific and specific immune responses and protect grass carp against GCRV infection, suggesting it could be used as an oral vaccine.

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