Salting‐out assisted liquid–liquid extraction with dansyl chloride for the determination of biogenic amines in food

2019 ◽  
Vol 55 (1) ◽  
pp. 248-258 ◽  
Author(s):  
Karen C. A. Francisco ◽  
Pedro F. Brandão ◽  
Rui Miguel Ramos ◽  
Luís Moreira Gonçalves ◽  
Arnaldo A. Cardoso ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Liquid Extraction ◽  
Dansyl Chloride ◽  
Salting Out ◽  
Liquid Liquid Extraction

Salting-out assisted liquid-liquid extraction method combined with GC-MS for the determination of topiramate in aqueous solutions: development and application of the methodology

2019 ◽  
Vol 55 (13) ◽  
pp. 2303-2312 ◽  
Author(s):  
Janiele Mayara Ferreira De Almeida ◽  
Elania Maria Fernandes Silva ◽  
Lourena Mafra Veríssimo ◽  
Nedja Suely Fernandes
Keyword(s):  
Aqueous Solutions ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Salting Out ◽  
Liquid Liquid Extraction

scholarly journals Salting-out assisted liquid–liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples

2019 ◽  
Vol 6 (8) ◽  
pp. 190952 ◽  
Author(s):  
Nur Hidayah Sazali ◽  
Anas Alshishani ◽  
Bahruddin Saad ◽  
Ker Yin Chew ◽  
Moi Me Chong ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin D3 ◽  
High Performance ◽  
Liquid Extraction ◽  
Sample Treatment ◽  
Salting Out ◽  
Milk Samples ◽  
Liquid Liquid Extraction

In this study, salting-out assisted liquid–liquid extraction (SALLE) as a simple and efficient extraction technique followed by high-performance liquid chromatography (HPLC) was employed for the determination of vitamin D3 in milk samples. The sample treatment is based on the use of water-miscible acetonitrile as the extractant and acetonitrile phase separation under high-salt conditions. Under the optimum conditions, acetonitrile and ammonium sulfate were used as the extraction solvent and salting-out agent, respectively. The vitamin D3 extract was separated using Hypersil ODS (250x i.d 4.6 mm, 5 µm) HPLC column that was coupled with diode array detector. Vitamin D2 was used as internal standard (IS) to offset any variations in chromatographic conditions. The vitamin D3 and the IS were eluted in 18 min. Good linearity ( r 2 > 0.99) was obtained within the range of 25–600 ng g −1 with the limit of detection of 15 ng g −1 and limit of quantification of 25 ng g −1 . The validated method was applied for the determination of vitamin D3 in milk samples. The recoveries for spiked samples were from 94.4 to 113.5%.

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