Trends in Clark Level and Breslow Thickness of Invasive Cutaneous Malignant Melanoma over a 50-Year Period in Olmsted County/Rochester, MN

2008 ◽  
Vol 32 (1) ◽  
pp. 109-109
Author(s):  
D. Pierson ◽  
A. Subtil ◽  
M. Pittelkow ◽  
C. Scott ◽  
R. Weenig ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Cutaneous Malignant Melanoma ◽  
Breslow Thickness ◽  
Olmsted County ◽  
Clark Level

scholarly journals Immunohistochemical Analysis of BRAF (V600E) Mutation and P16 Expression in Malignant Melanoma in Lagos, Nigeria: A 10-Year Retrospective Study

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
O. Obadofin ◽  
K. Badmos ◽  
N. Orsi ◽  
M. Bipin ◽  
O. Rotimi ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Protein Expression ◽  
Immunohistochemical Analysis ◽  
Braf V600e Mutation ◽  
Breslow Thickness ◽  
University Teaching ◽  
Braf V600e ◽  
Clark Level ◽  
P16 Protein ◽  
P16 Expression

Background. In Blacks, malignant melanoma (MM) is associated with greater morbidity and mortality compared to Caucasians. MMs with BRAF V600E mutation as well as those with loss of p16 protein expression are associated with aggressive behavior and worse prognosis. Objectives. We determined BRAF (V600E) mutation status and loss of p16 expression in MM cases in Lagos, Nigeria, and correlated these with histopathologic parameters and patients’ age. Methods. Forty-five cases of MM received between January 2005 and December 2014 in the Anatomic and Molecular Pathology Department of Lagos University Teaching Hospital were subjected to immunohistochemical studies to determine BRAF V600E mutation and p16 protein expression. These included cutaneous (n=37), musosal (n=3), and ocular MM (n=2) as well as lymph node metastatases (n=3). Results. BRAF (V600E) mutations were detected in 5/45 (11%) while 31/45 (69%) of the cases had loss of p16 expression. No statistically significant association was found between the BRAF (V600E) mutation, loss of p16 expression, and histologic parameters such as histologic variant, Clark level, Breslow thickness, and ulceration. Conclusion. BRAF (V600E) mutation was detected only in a small proportion of cases while loss of p16 expression occurred in most cases which also had high Clark level, high Breslow thickness, and ulceration.

scholarly journals HSP105 expression in cutaneous malignant melanoma: Correlation with clinicopathological characteristics

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258053
Author(s):  
Ke-Jun Chen ◽  
Feng-Zeng Li ◽  
Qian Ye ◽  
Meng Jia ◽  
Sheng Fang
Keyword(s):  
Heat Shock ◽  
Malignant Melanoma ◽  
Staining Intensity ◽  
Clinicopathological Characteristics ◽  
Cutaneous Malignant Melanoma ◽  
Breslow Thickness ◽  
Human Immune System ◽  
Significant Difference ◽  
Lentigo Maligna Melanoma ◽  
Shock Proteins

Background Heat shock proteins can protect against stress-associated cellular challenges, but they can also protect some tumors from human immune system monitoring. Heat shock protein 105 (HSP105/110) is a high molecular weight protein whose expression has been reported in many cancers, but few studies on its role in cutaneous malignant melanoma have been published. In this study, we analyzed the relationship between HSP105 expression and the clinicopathological characteristics of CMM. Methods This retrospective study included 91 patients with CMM. The clinicopathological characteristics of CMM patients, including age, lesion duration, location, pathological classification, Clark’s level, Breslow thickness, metastasis and recurrence, were collected. Immunohistochemical staining and Western blot analysis for HSP105 were performed. Pigmented nevi (n = 20) served as a control. The staining intensity and percentage of stained cells were expressed as a histochemical score (HSCORE). Results HSP105 was overexpressed in melanoma compared with nevi. Differences in the HSCORE between nevi (HSCORE = 1.05(0.15,1.50)) and CMM (HSCORE = 2.68(1.80,3.60)) were remarkable (P<0.001). Exposed site lesions, recurrent and metastatic lesions, nodular melanoma and lentigo maligna melanoma were closely associated with higher HSP105 expression (P = 0.011, P = 0.001 and P = 0.001, respectively). Moreover, no significant difference was observed in Clark’s level, Breslow thickness, or lesion duration (P>0.05). Conclusion HSP105 is overexpressed in CMM. Higher HSP105 expression in lesions is associated with different clinicopathological variables. HSP105 may be a potential target for the diagnosis, treatment and prognostic prediction of CMM.

scholarly journals Метастатическое поражение периферических лимфатических узлов при злокачественной меланоме кожи: роль эластографии сдвиговой волной

2017 ◽  
pp. 25-35
Keyword(s):  
Malignant Melanoma ◽  
Lymph Nodes ◽  
Shear Wave ◽  
Wave Velocity ◽  
Shear Wave Velocity ◽  
Shear Wave Elastography ◽  
Cutaneous Malignant Melanoma ◽  
Ultrasound Elastography ◽  
Clark Level ◽  
Level Of Invasion

С января по август 2017 г. выполнены ультразвуковые исследования лимфатических узлов у 51 человека (23 мужчины и 28 женщин) в возрасте 23-86 лет с цитологически верифицированным диагнозом “злокачественная меланома кожи”. У всех пациентов заподозрены метастатические изменения в лимфатических узлах различной локализации по данным серошкальной эхографии и допплерографических методик. Исследования были выполнены на аппарате Aplio 500 (Toshiba, Япония) линейным датчиком 7-18 МГц. В первую группу вошел 21 человек с метастазами в регионарных лимфатических узлах, во вторую - 30 пациентов с гиперплазией лимфоидноретикулярной ткани. Значения модуля Юнга для метастатически измененных лимфатических узлов составили 101,8 кПа (медиана), 14,0-164,5 кПа (2,5-97,5-й процентили), для гиперплазированных лимфатических узлов - 15,0 кПа, 7,2-56,8 кПа соответственно. Значения модуля Юнга в метастатически измененных лимфатических узлах не коррелировали ни с уровнем инвазии первичной опухоли (меланомы) по Кларку, ни с локализацией метастазов (подмышечные, шейные, паховые лимфатические узлы). Выявлены значимые различия в значениях модуля Юнга между метастатически измененными и гиперплазированными лимфатическими узлами у пациентов с меланомой кожи (P 0,0001). Наилучшие показатели диагностической информативности в выявлении метастатического поражения лимфатических узлов при меланоме кожи достигнуты при пороговом значении модуля Юнга 22,7 кПа (чувствительность - 86%, специфичность - 77%, AUC - 0,916). Ключевые слова: ультразвуковая эластография, эластография сдвиговой волной, злокачественная меланома кожи, уровень инвазии по Кларку, толщина по Бреслоу, лимфатические узлы, модуль Юнга, скорость сдвиговой волны, ultrasound elastography, shear wave elastography, cutaneous malignant melanoma, Clark level of invasion, Breslow thickness, lymph nodes, Young’s modulus, shear wave velocity

Sign in / Sign up

Export Citation Format

Share Document