Bacterial contaminants of micropropagated plant cultures

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency

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Faculty Opinions recommendation of Characterization of bacterial contaminants in the air of a duck hatchery by cultivation based and molecular methods.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.8497956.8969054 ◽

2011 ◽

Author(s):

Monika Raulf ◽

Verena Liebers

Keyword(s):

Molecular Methods ◽

Bacterial Contaminants

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ELIMINATION OF BACTERIAL CONTAMINANTS: A MATTER OF DETECTION AND TRANSPLANTING PROCEDURES

Acta Horticulturae ◽

10.17660/actahortic.1988.225.20 ◽

1988 ◽

pp. 183-188 ◽

Cited By ~ 8

Author(s):

B.P.A.M. Kunneman ◽

G.P.M. Faaij-Groenen

Keyword(s):

Bacterial Contaminants

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Optimized genotyping method for identification of bacterial contaminants in pharmaceutical industry

Acta Pharmaceutica ◽

10.1515/acph-2016-0011 ◽

2016 ◽

Vol 66 (2) ◽

pp. 289-295

Author(s):

Borche Stamatoski ◽

Miroslava Ilievska ◽

Hristina Babunovska ◽

Nikola Sekulovski ◽

Sasho Panov

Keyword(s):

Pharmaceutical Industry ◽

Dna Sequencing ◽

Bacterial Contamination ◽

Raw Materials ◽

Pcr Primers ◽

Rrna Gene ◽

Bacterial Identification ◽

Bacterial Contaminants ◽

Microbiological Control ◽

Selection Of

AbstractMicrobiological control is of crucial importance in the pharmaceutical industry regarding the possible bacterial contamination of the environment, water, raw materials and finished products. Molecular identification of bacterial contaminants based on DNA sequencing of the hypervariable 16SrRNA gene has been introduced recently. The aim of this study is to investigate the suitability of gene sequencing using our selection of PCR primers and conditions for rapid and accurate bacterial identification in pharmaceutical industry quality control.DNA was extracted from overnight incubated colonies from 10 bacterial ATCC strains, which are common contaminants in the pharmaceutical industry. A region of bacterial 16SrRNA gene was analyzed by bidirectional DNA sequencing. Bacterial identification based on partial sequencing of the 16SrRNA gene is the appropriate method that could be used in the pharmaceutical industry after adequate validations. We have successfully identified all tested bacteria with more than 99 % similarity to the already published sequences.

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A METHOD OF ISOLATING ACTINOMYCETES FROM SCABBY POTATO TISSUE AND SOIL WITH MINIMAL CONTAMINATION

Canadian Journal of Botany ◽

10.1139/b56-005 ◽

1956 ◽

Vol 34 (1) ◽

pp. 44-47 ◽

Cited By ~ 37

Author(s):

C. H. Lawrence

Keyword(s):

Culture Medium ◽

Potato Tubers ◽

Sodium Propionate ◽

Potato Tissue ◽

Treated Soil ◽

Bacterial Contaminants ◽

Fungal Contaminants

The isolation of actinomycetes from soil or from scabby potato tubers was facilitated by a 10-min. treatment of the material from which the isolations were to be made with phenol in dilution of 1: 140. This eliminated most bacterial contaminants and reduced fungal contaminants especially those of the spreading type. Treatment of the material with higher concentrations of phenol progressively decreased the number of actinomycetes until there was no growth after treatment with 1: 70 dilution of phenol. Optimum development and maximum numbers of actinomycetes occurred when phenol-treated material was cultured on media adjusted to pH 6.5. More actinomycetes developed on glucose-asparagine agar than on Czapek's agar inoculated with phenol-treated material from scab-infected potatoes. However, when phenol-treated soil suspensions were tested, Czapek's agar was more favorable to the development of actinomycete colonies. A comparison of the phenol method with another in which sodium propionate is incorporated into the culture medium showed that the phenol method was more efficient in reducing contaminants and in permitting a larger number of actinomycete colonies to develop.

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