Evaluation of surface sterilization and hot water treatments on bacterial contaminants in bud culture of sugarcane

1999 ◽  
Vol 35 (3) ◽  
pp. 265-274 ◽  
Author(s):  
M. MOUTIA ◽  
A. DOOKUN
Keyword(s):  
Hot Water ◽  
Surface Sterilization ◽  
Bacterial Contaminants ◽  
Water Treatments

scholarly journals Disinfection of Orange Seeds to Reduce Citrus Canker Dissemination and Improve Germination

2020 ◽  
Vol 8 (4) ◽  
pp. 372
Author(s):  
Asma Akbar ◽  
Shehzad Asad ◽  
Zahoor Ahmad ◽  
Azra Nadeem ◽  
Shehla Awan
Keyword(s):  
Water Treatment ◽  
Bacterial Contamination ◽  
Germination Rate ◽  
Citrus Canker ◽  
Hot Water ◽  
Hot Water Treatment ◽  
Surface Sterilization ◽  
Bacterial Inoculum ◽  
Water Treatments ◽  
Important Disease

We evaluated chemo and thermotherapy for decontaminating orange seeds against the bacteria Xanthomonas citri subsp. citri (Xcc), a quarantine and economically important disease worldwide. We infected orange (cv. Valencia) seeds with bacterial inoculum and studied the potential of three different methods i.e. surface sterilization with bleach, disinfection with plant preservative mixture, and hot water treatment to eliminate the bacteria. Our findings showed that the hot water, bleach (NaOCl), and a commercially available mixture of antifungal and antibacterial compounds known as Plant Preservative Mixture (PPM) were very effective in eliminating most bacterial contamination from orange seeds. Our results also showed that treatment with bleach and hot water treatment enhanced seed germination and seedling vigor. Germination rate ranged from 18.51 to 77.78%, 7.41 to 62.96% and 13.33 to 66.67% for bleach, PPM and hot water treatments, respectively. Seedling height ranged from 4.28 to 6.63cm, 4.17 to 5.94cm and 3.42 to 5.99cm for bleach, PPM and hot water treatment, respectively. Findings of this study will provide impactful and practical methods for an economical and robust seed decontamination method.

Alfa fibers for Cereplast bio-composites reinforcement: Effects of chemical and biological treatments on the mechanical properties

2021 ◽  
pp. 096739112110060
Author(s):  
Mouna Werchefani ◽  
Catherine Lacoste ◽  
Hafedh Belguith ◽  
Chedly Bradai
Keyword(s):  
Charpy Impact ◽  
Impact Testing ◽  
Hot Water ◽  
Cellulose Content ◽  
Twin Screw ◽  
Electron Microscopy Analysis ◽  
Alfa Fibers ◽  
Charpy Impact Testing ◽  
Water Treatments ◽  
The Impact

The present work is a comparative study of the impact of Alfa fiber modifications on the Cereplast composites mechanical behavior. Various treatments have been employed, including mechanical, soda, saltwater-retting, hot-water treatments and enzymatic treatment using xylanase. Chemical and morphological analyses were carried out in order to determine the changes of the biochemical composition and the dimensions of fibers. Cereplast composites reinforced with Alfa fibers were fabricated using a twin-screw extrusion followed by an injection molding technique with a fiber load of 20 wt. %. Resulting materials were assessed by means of tensile, flexural and Charpy impact testing. Scanning Electron Microscopy analysis was carried out to investigate the interfacial properties of the composites. The results have shown a significant enhancement of mechanical strengths and rigidities for the xylanase-treated fiber composites, owing to the increase of cellulose content, the enhancement of defibrillation level and the improvement of matrix-fiber adhesion. The data proved that the technology of enzymes can be used as a powerful and eco-friendly approach to modify fiber surfaces and to increase their potential of reinforcement.

Evaluation of Hot Water and Electron Beam Irradiation for Reducing Fusarium Infection in Malting Barley

2003 ◽  
Vol 66 (7) ◽  
pp. 1241-1246 ◽  
Author(s):  
BALASUBRAHMANYAM KOTTAPALLI ◽  
CHARLENE E. WOLF-HALL ◽  
PAUL SCHWARZ ◽  
JURGEN SCHWARZ ◽  
JAMES GILLESPIE
Keyword(s):  
Electron Beam ◽  
Product Quality ◽  
Electron Beam Irradiation ◽  
Hot Water ◽  
Slight Reduction ◽  
Malting Barley ◽  
Beam Irradiation ◽  
Physical Methods ◽  
Fusarium Infection ◽  
Water Treatments

The use of Fusarium-infected barley for malting may lead to mycotoxin production and decreased product quality. Physical methods for the treatment of Fusarium-infected barley may prevent these safety and quality defects and allow the use of otherwise good quality barley. Hot water and electron beam irradiation were evaluated for their effectiveness in reducing Fusarium infection while maintaining germinative energy in barley samples. Hot-water treatments involved temperatures of 45, 50, 55, and 60°C and treatment times of 0, 1, 5, 10, and 15 min. Electron beam irradiation involved doses ranging from 0 to 11.4 kGy. Treatment with water at 45°C for 15 min resulted in a reduction in Fusarium infection from 32 to 1% after 15 min, with only a very slight reduction in germination. Treatment with water at 50°C for 1 min resulted in a reduction in Fusarium infection from 32 to 2%, and no effect on germination was observed for up to 5 min of treatment. At higher water temperatures, Fusarium infection was essentially eliminated, but germination was also severely reduced. Electron beam irradiation of Fusarium-infected barley reduced Fusarium infection at doses of >4 kGy, and a slight increase in germination for dry samples was observed with doses of 6 to 8 kGy. Doses of >10 kGy significantly decreased germination. Physical methods may have potential for the treatment of Fusarium-infected malting barley.

scholarly journals Breaking Dormancy in Red Raspberry with Hot Water Treatment and its Effects on Cold Hardiness

2006 ◽  
Vol 131 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Pauliina Palonen ◽  
Leena Lindén
Keyword(s):  
Water Treatment ◽  
Cold Hardiness ◽  
Treatment Temperature ◽  
Rubus Idaeus ◽  
Hot Water ◽  
Bud Dormancy ◽  
Hot Water Treatment ◽  
Red Raspberry ◽  
Breaking Dormancy ◽  
Water Treatments

`Maurin Makea', `Muskoka', ` Ottawa', and `Preussen' red raspberry (Rubus idaeus L.) canes were collected from the field and subjected to different hot water treatments (20, 35, 40, 45, and 50 °C) to determine if endodormancy could be removed by a near lethal stress. Estimation of days for 50% budbreak (DD50) was found useful for describing the state of bud dormancy in the samples. Bud dormancy was broken in `Ottawa' by immersing the canes in 45 °C water for 2 hours, in `Maurin Makea' by treating the canes in 40 °C water, and in `Preussen' by both 40 and 45 °C treatments. The influence of this treatment on dormancy and cold hardiness at different times of the winter was further examined using `Ottawa' raspberry. The treatment removed bud dormancy most effectively in October, when the samples were in deepest dormancy. A slight effect was observed in November, but no effect in January. During ecodormancy in February the treatment delayed budbreak. Hot water treatment reduced cold hardiness of `Ottawa' canes by 8 to 15 °C, and that of buds by 9 to 13 °C during both endo- and ecodormancy. Based on the capacity of buds and canes to reacclimate, recovery from the stress treatment was possible at temperatures ≥4 °C. Loss of cold hardiness was caused by high treatment temperature itself and was not related to breaking of dormancy in samples. This finding suggests that dormancy and cold hardiness are physiologically unconnected in raspberry.

EFFECTS OF HOT WATER TREATMENTS ON POSTHARVEST QUALITY AND ETHYLENE SYNTHESIS OF BANANAS

1998 ◽  
pp. 529-536 ◽  
Author(s):  
A. Marrero Domínguez ◽  
J.J. López Cabrera ◽  
M. Pomar García
Keyword(s):  
Hot Water ◽  
Postharvest Quality ◽  
Ethylene Synthesis ◽  
Water Treatments

The effectiveness of Virkon® S disinfectant against an invasive insect and implications for Antarctic biosecurity practices

2020 ◽  
pp. 1-9 ◽  
Author(s):  
Jesamine C. Bartlett ◽  
Richard James Radcliffe ◽  
Pete Convey ◽  
Kevin A. Hughes ◽  
Scott A.L. Hayward
Keyword(s):  
Salt Water ◽  
Hot Water ◽  
Antarctic Region ◽  
Invasive Insect ◽  
Signy Island ◽  
Antarctic Treaty ◽  
Biosecurity Practices ◽  
Water Treatments ◽  
The Antarctic ◽  
Maximum Threshold

Abstract The flightless midge Eretmoptera murphyi is thought to be continuing its invasion of Signy Island via the treads of personnel boots. Current boot-wash biosecurity protocols in the Antarctic region rely on microbial biocides, primarily Virkon® S. As pesticides have limited approval for use in the Antarctic Treaty area, we investigated the efficacy of Virkon® S in controlling the spread of E. murphyi using boot-wash simulations and maximum threshold exposures. We found that E. murphyi tolerates over 8 h of submergence in 1% Virkon® S. Higher concentrations increased effectiveness, but larvae still exhibited > 50% survival after 5 h in 10% Virkon® S. Salt and hot water treatments (without Virkon® S) were explored as possible alternatives. Salt water proved ineffective, with mortality only in first-instar larvae across multi-day exposures. Larvae experienced 100% mortality when exposed for 10 s to 50°C water, but they showed complete survival at 45°C. Given that current boot-wash protocols alone are an ineffective control of this invasive insect, we advocate hot water (> 50°C) to remove soil, followed by Virkon® S as a microbial biocide on ‘clean’ boots. Implications for the spread of invasive invertebrates as a result of increased human activity in the Antarctic region are discussed.

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