A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues

2009 ◽  
Vol 235 (3) ◽  
pp. 273-281 ◽  
Author(s):  
K.L. MCDONALD
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Light And Electron Microscopy ◽  
High Pressure Freezing ◽  
Preparation Techniques

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Morphology of the lateral fields of the infective juvenile of the entomopathogenic family Steinernematidae (Nematoda) using high pressure freezing (HPF)

Nematology ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 69-74
Author(s):  
Zdeněk Mráček ◽  
Jiří Nermut’ ◽  
Martina Tesařová ◽  
Vladimír Půža
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Pressure ◽  
Infective Juvenile ◽  
Field Pattern ◽  
Taxonomic Character ◽  
High Pressure Freezing ◽  
Lateral Field ◽  
Transmission Electron ◽  
Scanning Electron

Summary The lateral field pattern of infective juveniles of the nematode family Steinernematidae is an important taxonomic character. Scanning electron microscopy (SEM) shows the number of ridges and lines or incisures clearly, but does not provide other details. In the present study, ten species from six clades of Steinernematidae have been studied for their lateral field morphology using SEM and high pressure freezing (HPF) with transmission electron microscopy (TEM). Both methods indicated the same number of ridges and lines, although HPF/TEM resulted in a more detailed morphology with differences between the species. The tips of the ridges are either finely rounded or pointed and the lines are V-shaped or have a broadened bottom. These characters represent an additional pattern that may be characteristic for some species within the phylogenetic clades. Further studies of the lateral field morphology of other species is needed to ascertain whether each pattern is clade specific and phylogenetically valuable.

scholarly journals Lamellar body ultrastructure revisited: high-pressure freezing and cryo-electron microscopy of vitreous sections

2010 ◽  
Vol 134 (4) ◽  
pp. 319-326 ◽  
Author(s):  
Dimitri Vanhecke ◽  
Gudrun Herrmann ◽  
Werner Graber ◽  
Therese Hillmann-Marti ◽  
Christian Mühlfeld ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Lamellar Body ◽  
High Pressure Freezing ◽  
Cryo Electron Microscopy

scholarly journals PB-10 High-pressure freezing method can be replaced by sandwich freezing method !?: electron microscopy of human tissues and cultured cells

Microscopy ◽  
2019 ◽  
Vol 68 (Supplement_1) ◽  
pp. i50-i50
Author(s):  
Masashi Yamaguchi ◽  
Seiichiro Wakabayashi ◽  
Yuumi Nakamura ◽  
Hiroyuki Matsue ◽  
Takuya Hirao ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Cultured Cells ◽  
Human Tissues ◽  
High Pressure Freezing ◽  
Freezing Method

Electron microscopy analysis of maize basal endosperm transfer cells processed by high-pressure freezing and freeze-substitution

2008 ◽  
Vol 14 (S2) ◽  
pp. 1502-1503
Author(s):  
B-H Kang ◽  
D Williams ◽  
K Kelley ◽  
K Backer-Kelley ◽  
P Chourey
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
New Mexico ◽  
Freeze Substitution ◽  
Transfer Cells ◽  
High Pressure Freezing ◽  
Endosperm Transfer Cells ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

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