Somatostatin-lmmunoreactive Neurones in the Hypothalamic Ventromedial Nucleus Possess Oestrogen Receptors in the Male and Female Rat

ABSTRACT The effects of various doses of testosterone propionate (TP) upon the release of luteinizing hormone (LH or ICSH) from the hypophysis of a gonadectomized male or female rat were compared. Prostate weight in hypophysectomized male parabiotic partners was used to evaluate the quantity of circulating LH. Hypophyseal LH was measured by the ovarian ascorbic acid depletion method. Males castrated when 45 days old secreted significantly more LH and had three times the amount of pituitary LH as ovariectomized females. Administration of 25 μg TP daily reduced the amount of LH in the plasma, and increased the amount in the pituitary gland, in both sexes. Treatment with 50 μg caused a further reduction in plasma LH in males, but not in females, while pituitary levels in both were equal to that of their respective controls. LH fell to the same low level in partners of males or females receiving 100 μg TP. When gonadectomized at 39 days, males and females had the same amount of plasma LH, but males had more stored hormone. Pituitary levels were unchanged from controls following treatment with 12.5, 25 or 50 μg TP daily, but plasma values dropped an equal amount in both sexes with the latter two doses. Androgenized males or females, gonadectomized when 39 days old, were very sensitive to the effects of TP and plasma LH was significantly reduced with 12.5 μg daily. Pituitary LH in androgenized males was higher than that of normal males but was reduced to normal by small amounts of TP. The amount of stored LH in androgenized females was not different from that of normal females and it was unchanged by any dose of TP tested. Results are consistent with the conclusion that the male hypothalamic-hypophyseal axis is at least as sensitive as the female axis to the negative feedback effects of TP. Androgenization increases the sensitivity to TP in both males and females.

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170 Short-term Exposure to High Glucose Alters Acetylcholine Sensitivity and Maximal Relaxation in Male and Female Rat Pudendal Artery, Respectively, Which is Reversed With Testosterone Incubation

Journal of Sexual Medicine ◽

10.1016/j.jsxm.2017.11.129 ◽

2018 ◽

Vol 15 (2) ◽

pp. S51-S52

Author(s):

N.S. Klee ◽

C. Webb

Keyword(s):

High Glucose ◽

Female Rat ◽

Short Term ◽

Male And Female ◽

Short Term Exposure ◽

Acetylcholine Sensitivity

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The role of adrenoceptors in the central nervous system in male and female rat sexual behavior

European Journal of Pharmacology ◽

10.1016/j.ejphar.2014.09.003 ◽

2015 ◽

Vol 753 ◽

pp. 229-245 ◽

Cited By ~ 10

Author(s):

Eelke M.S. Snoeren

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Sexual Behavior ◽

Female Rat ◽

Male And Female ◽

The Central Nervous System

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Glutathione Conjugates of Ochratoxin a as Biomarkers of Exposure / Glutationski Konjugati Okratoksina A Kao Biomarkeri Izloženosti

Archives of Industrial Hygiene and Toxicology ◽

10.2478/10004-1254-63-2012-2202 ◽

2012 ◽

Vol 63 (4) ◽

pp. 417-427 ◽

Cited By ~ 27

Author(s):

Mariana Tozlovanu ◽

Delphine Canadas ◽

Annie Pfohl-Leszkowicz ◽

Christine Frenette ◽

Robert J. Paugh ◽

...

Keyword(s):

Ochratoxin A ◽

Rat Kidney ◽

Female Rats ◽

Amide Bond ◽

Male Rats ◽

Dark Agouti ◽

Female Rat ◽

Male And Female ◽

Male And Female Rats ◽

Liver And Kidney

AbstractIn the present study the photoreactivity of the fungal carcinogen ochratoxin A (OTA) has been utilised to generate authentic samples of reduced glutathione (GSH) and N-acetylcysteine (NAC) conjugates of the parent toxin. These conjugates, along with the nontoxic OTα, which is generated through hydrolysis of the amide bond of OTA by carboxypeptidase A, were utilised as biomarkers to study the metabolism of OTA in the liver and kidney of male and female Dark Agouti rats. Male rats are more susceptible than female rats to OTA carcinogenesis with the kidney being the target organ. Our studies show that the distribution of OTA in male and female rat kidney is not significantly different. However, the extent of OTA metabolism was greater in male than female rats. Much higher levels of OTα were detected in the liver compared to the kidney, and formation of OTα is a detoxification pathway for OTA. These findings suggest that differences in metabolism between male and female rats could provide an explanation for the higher sensitivity of male rats to OTA toxicity

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Intrasplenic Transplantation of Isolated Adult Rat Hepatocytes

Toxicologic Pathology ◽

10.1177/0192623311425061 ◽

2011 ◽

Vol 40 (1) ◽

pp. 83-92 ◽

Cited By ~ 5

Author(s):

Meena R. Sharma ◽

Wojciech Dworakowski ◽

Bernard H. Shapiro

Keyword(s):

Cytochrome P450 ◽

Adult Male ◽

Total Concentration ◽

Rat Hepatocytes ◽

Female Rats ◽

Female Rat ◽

Male And Female ◽

Sexual Dimorphisms ◽

Male And Female Rats ◽

Male Specific

Adult male and female rat hepatocytes were individually transplanted into the spleens of adult male and female rats. The recipients were euthanized at either eight, sixteen, thirty, or forty-five weeks following transplantation, at which time hepatic and splenic levels of liver-specific rat albumin mRNA as well as sex-dependent transcript levels of CYP2C11, -2C12, -2C7, -2A1, and -3A2—which accounts for > 60% of the total concentration of hepatic constituent cytochrome P450—were determined. Whereas the pre-infused hepatocytes expressed their expected cytochrome P450 sexual dimorphisms (female-specific CYP2C12, male-specific CYP3A2, and female-predominant CYP2A1), their post-transplantational competence now reflected the sexual dimorphisms of the recipient (as observed in the host’s liver), which supports the concept that the sex-dependent growth hormone circulating profiles are the determinants regulating the expression levels of hepatic cytochrome P450. Also expressed at normal concentrations in the pre-infused hepatocytes, male-specific CYP2C11 and female-predominant CYP2C7 were inexplicably undetectable in the spleens of both recipient males and females, regardless of the sex of the donor hepatocytes, almost one year after transplantation.

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