scholarly journals Identification and characterization of three novel EsaI/EsaR quorum-sensing controlled stewartan exopolysaccharide biosynthetic genes inPantoea stewartiissp. stewartii

2009 ◽  
Vol 74 (4) ◽  
pp. 903-913 ◽  
Author(s):  
Aurélien Carlier ◽  
Lindsey Burbank ◽  
Susanne B. von Bodman
Keyword(s):  
Quorum Sensing ◽  
Biosynthetic Genes ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Quorum-Quenching Activity of N-Acylhomoserine Lactonase from Coagulase-Negative Staphylococci

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 483
Author(s):  
Tomohiro Morohoshi ◽  
Yaoki Kamimura ◽  
Nobutaka Someya
Keyword(s):  
Quorum Sensing ◽  
Opportunistic Pathogen ◽  
Quorum Quenching ◽  
Coagulase Negative Staphylococci ◽  
Sensing Systems ◽  
Genes Encoding ◽  
Acyl Chains ◽  
Gene Homologue ◽  
Identification And Characterization

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signals in Gram-negative bacteria. Many genes encoding AHL-degrading enzymes have been cloned and characterized in various microorganisms. Coagulase-negative staphylococci (CNS) are present on the skin of animals and are considered low-virulent species. The AHL-lactonase gene homologue, ahlS, was present in the genomes of the CNS strains Staphylococcus carnosus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, and Staphylococcus sciuri. We cloned the candidate ahlS homologue from six CNS strains into the pBBR1MCS5 vector. AhlS from the CNS strains showed a higher degrading activity against AHLs with short acyl chains compared to those with long acyl chains. AhlS from S. sciuri was expressed and purified as a maltose-binding protein (MBP) fusion. Pseudomonas aeruginosa is an opportunistic pathogen that regulates several virulence factors such as elastase and pyocyanin by quorum-sensing systems. When MBP-AhlS was added to the culture of P. aeruginosa PAO1, pyocyanin production and elastase activity were substantially reduced compared to those in untreated PAO1. These results demonstrate that the AHL-degrading activity of AhlS from the CNS strains can inhibit quorum sensing in P. aeruginosa PAO1.

Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas

2019 ◽  
Vol 170 (6-7) ◽  
pp. 243-255 ◽  
Author(s):  
Zhiliang Yu ◽  
Dongliang Yu ◽  
Yanfeng Mao ◽  
Mengting Zhang ◽  
Mengdan Ding ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Sensing System ◽  
Quorum Sensing System ◽  
Identification And Characterization

Identification and characterization of naturally occurring DSF-family quorum sensing signal turnover system in the phytopathogenXanthomonas

2015 ◽  
Vol 17 (11) ◽  
pp. 4646-4658 ◽  
Author(s):  
Lian Zhou ◽  
Xing-Yu Wang ◽  
Shuang Sun ◽  
Li-Chao Yang ◽  
Bo-Le Jiang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Naturally Occurring ◽  
Identification And Characterization

scholarly journals Quorum-Sensing Molecules: Sampling, Identification and Characterization of N-Acyl-Homoserine Lactone in Vibrio sp

2022 ◽  
Author(s):  
Noha Laj ◽  
Muhammed Elayadeth-Meethal ◽  
V. Aldous J. Huxley ◽  
Raishy R. Hussain ◽  
Mohamed Saheer Kuruniyan ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Phenylpropanoid Biosynthetic Genes and Their Accumulation in Bitter Melon (Momordica charantia)

Molecules ◽  
2018 ◽  
Vol 23 (2) ◽  
pp. 469 ◽  
Author(s):  
Do Cuong ◽  
Soon-Jae Kwon ◽  
Jin Jeon ◽  
Yun Park ◽  
Jong Park ◽  
...  
Keyword(s):  
Momordica Charantia ◽  
Bitter Melon ◽  
Biosynthetic Genes ◽  
Identification And Characterization

scholarly journals Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon

1998 ◽  
Vol 335 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Evelyne RAUX ◽  
Anne LANOIS ◽  
Martin J. WARREN ◽  
Alain RAMBACH ◽  
Claude THERMES
Keyword(s):  
Bacillus Megaterium ◽  
Genomic Library ◽  
Early Stage ◽  
Open Reading Frames ◽  
Biosynthetic Genes ◽  
Pseudomonas Denitrificans ◽  
Dna Fragment ◽  
Identification And Characterization ◽  
Synechocystis Sp

A 16 kb DNA fragment has been isolated from a Bacillus megaterium genomic library and fully sequenced. The fragment contains 15 open reading frames, 14 of which are thought to constitute a B. megaterium cobalamin biosynthetic (cob) operon. Within the operon, 11 genes display similarity to previously identified Salmonella typhimurium cobalamin biosynthetic genes (cbiH60, -J, -C, -D, -ET, -L, -F, -G, -A, cysGA and btuR), whereas three do not (cbiW, -X and -Y). The genes of the B. megaterium cob operon were compared with the cobalamin biosynthetic genes of Pseudomonas denitrificans, Methanococcus jannaschii and Synechocystis sp. Taking into account the presence of cbiD and cbiG, the absence of a cobF, cobG and cobN, -S and -T, it was concluded that B. megaterium, M. jannaschii and Synechocystis sp., like S. typhimurium, synthesize cobalamin by an anaerobic pathway, in which cobalt is added at an early stage and molecular oxygen is not required.

Identification and characterization of xanthone biosynthetic genes contributing to the vivid red coloration of red‐flowered gentian

2021 ◽  
Author(s):  
Nobuhiro Sasaki ◽  
Keiichirou Nemoto ◽  
Yuzo Nishizaki ◽  
Naoki Sugimoto ◽  
Keisuke Tasaki ◽  
...  
Keyword(s):  
Biosynthetic Genes ◽  
Identification And Characterization ◽  
Red Coloration
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