Quorum-Sensing Molecules: Sampling, Identification and Characterization of N-Acyl-Homoserine Lactone in Vibrio sp

Abstract Objectives To characterize the bacterial community of Wind Cave’s Madison aquifer through whole-genome sequencing, and to better understand the bacterial ecology by identifying genes involved in acyl-homoserine lactone (AHL) based quorum-sensing (QS) systems. Results Genome-based taxonomic classification revealed the microbial richness present in the pristine Madison aquifer. The strains were found to span eleven genera and fourteen species, of which eight had uncertain taxonomic classifications. The genomes of strains SD129 and SD340 were found to contain the archetypical AHL QS system composed of two genes, luxI and luxR. Surprisingly, the genomes of strains SD115, SD129, SD274 and SD316 were found to contain one to three luxR orphans (solos). Strain SD129, besides possessing an archetypical AHL QS luxI-luxR pair, also contained two luxR solos, while strain SD316 contained three LuxR solos and no luxI-luxR pairs. The ligand-binding domain of two LuxR solos, one each from strains SD129 and SD316, were found to contain novel substitutions not previously reported, thus may represent two LuxR orphans that detection and response to unknown self-produced signal(s), or to signal(s) produced by other organisms.

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Characterization of a luxI/luxR-type quorum sensing system and N-acyl-homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1

Research in Microbiology ◽

10.1016/j.resmic.2006.11.008 ◽

2007 ◽

Vol 158 (2) ◽

pp. 150-158 ◽

Cited By ~ 40

Author(s):

Rob Van Houdt ◽

Pieter Moons ◽

Abram Aertsen ◽

An Jansen ◽

Kristof Vanoirbeek ◽

...

Keyword(s):

Quorum Sensing ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone ◽

Serratia Plymuthica ◽

Sensing System ◽

Quorum Sensing System ◽

Component Production

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N-acyl homoserine lactone molecules assisted quorum sensing: effects consequences and monitoring of bacteria talking in real life

Archives of Microbiology ◽

10.1007/s00203-021-02381-9 ◽

2021 ◽

Author(s):

Ömür Acet ◽

Demet Erdönmez ◽

Burcu Önal Acet ◽

Mehmet Odabaşı

Keyword(s):

Quorum Sensing ◽

Real Life ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone

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Biofilm Formation, Communication and Interactions of Mesophilic Leaching Bacteria during Pyrite Oxidation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.825.107 ◽

2013 ◽

Vol 825 ◽

pp. 107-110

Author(s):

Sören Bellenberg ◽

Robert Barthen ◽

Mario Vera ◽

Nicolas Guiliani ◽

Wolfgang Sand

Keyword(s):

Quorum Sensing ◽

Acidithiobacillus Ferrooxidans ◽

Biofilm Formation ◽

Pyrite Oxidation ◽

Cell Communication ◽

Homoserine Lactone ◽

Mixed Cultures ◽

Acyl Homoserine Lactone ◽

Leaching Bacteria ◽

Leaching Efficiency

A functional luxIR-type Quorum Sensing (QS) system is present in Acidithiobacillus ferrooxidans. However, cell-cell communication among various acidophilic chemolithoautotrophs growing on pyrite has not been studied in detail. These aspects are the scope of this study with emphasis on the effects exerted by the N-acyl-homoserine lactone (AHL) type signaling molecules which are produced by Acidithiobacillus ferrooxidans. Their effects on attachment and leaching efficiency by other leaching bacteria, such as Acidithiobacillus ferrivorans, Acidiferrobacter spp. SPIII/3 and Leptospirillum ferrooxidans in pure and mixed cultures growing on pyrite is shown.

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Influence of Glucose Concentrations on Biofilm Formation, Motility, Exoprotease Production, and Quorum Sensing in Aeromonas hydrophila

Journal of Food Protection ◽

10.4315/0362-028x.jfp-12-321 ◽

2013 ◽

Vol 76 (2) ◽

pp. 239-247 ◽

Cited By ~ 51

Author(s):

IQBAL KABIR JAHID ◽

NA-YOUNG LEE ◽

ANNA KIM ◽

SANG-DO HA

Keyword(s):

Quorum Sensing ◽

Aeromonas Hydrophila ◽

Biofilm Formation ◽

Opportunistic Pathogen ◽

Homoserine Lactone ◽

Protease Production ◽

Acyl Homoserine Lactone ◽

Human Pathogen ◽

Initial Biofilm ◽

Motility Inhibition

Aeromonas hydrophila recently has received increased attention because it is opportunistic and a primary human pathogen. A. hydrophila biofilm formation and its control are a major concern for food safety because biofilms are related to virulence. Therefore, we investigated biofilm formation, motility inhibition, quorum sensing, and exoprotease production of this opportunistic pathogen in response to various glucose concentrations from 0.05 to 2.5% (wt/vol). More than 0.05% glucose significantly impaired (P < 0.05) quorum sensing, biofilm formation, protease production, and swarming and swimming motility, whereas bacteria treated with 0.05% glucose had activity similar to that of the control (0% glucose). A stage shift biofilm assay revealed that the addition of glucose (2.5%) inhibited initial biofilm formation but not later stages. However, addition of quorum sensing molecules N-3-butanoyl-DL-homoserine lactone and N-3-hexanoyl homoserine lactone partially restored protease production, indicating that quorum sensing is controlled by glucose concentrations. Thus, glucose present in food or added as a preservative could regulate acyl-homoserine lactone quorum sensing molecules, which mediate biofilm formation and virulence in A. hydrophila.

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Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

mBio ◽

10.1128/mbio.00398-17 ◽

2017 ◽

Vol 8 (3) ◽

Cited By ~ 13

Author(s):

Rita S. Valente ◽

Pol Nadal-Jimenez ◽

André F. P. Carvalho ◽

Filipe J. D. Vieira ◽

Karina B. Xavier

Keyword(s):

Signal Transduction ◽

Quorum Sensing ◽

Plant Pathogen ◽

Bacterial Species ◽

Homoserine Lactone ◽

Signal Molecules ◽

Acyl Homoserine Lactone ◽

Interspecies Interactions ◽

Major Factors

ABSTRACT Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks—the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway—control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants. We show that the AHL system controls the Gac/Rsm system by affecting the expression of the regulatory RNA RsmB. This regulation is mediated by ExpR2, the quorum-sensing receptor that responds to the P. wasabiae cognate AHL but also to AHLs produced by other bacterial species. As a consequence, this level of regulation allows P. wasabiae to bypass the Gac-dependent regulation of RsmB in the presence of exogenous AHLs or AHL-producing bacteria. We provide in vivo evidence that this pivotal role of RsmB in signal transduction is important for the ability of P. wasabiae to induce virulence in response to other AHL-producing bacteria in multispecies plant lesions. Our results suggest that the signaling architecture in P. wasabiae was coopted to prime the bacteria to eavesdrop on other bacteria and quickly join the efforts of other species, which are already exploiting host resources. IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae. This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways. IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae. This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways.

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