scholarly journals The scaffolding and signalling functions of a localization factor impact polar development

2012 ◽  
Vol 84 (4) ◽  
pp. 712-735 ◽  
Author(s):  
Patrick D. Curtis ◽  
Ellen M. Quardokus ◽  
Melanie L. Lawler ◽  
Xiaoyun Guo ◽  
David Klein ◽  
...  
Keyword(s):  
Localization Factor ◽  
Polar Development

scholarly journals Identification of Genes Required for Synthesis of the Adhesive Holdfast in Caulobacter crescentus

2003 ◽  
Vol 185 (4) ◽  
pp. 1432-1442 ◽  
Author(s):  
Chris S. Smith ◽  
Aaron Hinz ◽  
Diane Bodenmiller ◽  
David E. Larson ◽  
Yves V. Brun
Keyword(s):  
Outer Membrane ◽  
Gene Cluster ◽  
Xanthomonas Campestris ◽  
Caulobacter Crescentus ◽  
Sequence Similarity ◽  
Gram Negative ◽  
Transmission Electron ◽  
Polar Development ◽  
Transposon Insertions ◽  
Polar Organelle

ABSTRACT Adhesion to both abiotic and biotic surfaces by the gram-negative prothescate bacterium Caulobacter crescentus is mediated by a polar organelle called the “holdfast,” which enables the bacterium to form stable monolayer biofilms. The holdfast, a complex polysaccharide composed in part of N-acetylglucosamine, localizes to the tip of the stalk (a thin cylindrical extension of the cell wall and membranes). We report here the isolation of adhesion mutants with transposon insertions in an uncharacterized gene cluster involved in holdfast biogenesis (hfs) as well as in previously identified polar development genes (podJ and pleC), and the holdfast attachment genes (hfa). Clean deletions of three of the four genes in the hfs gene cluster (hfsDAB) resulted in a severe holdfast biogenesis phenotype. These mutants do not bind to surfaces or to a fluorescently labeled lectin, specific for N-acetylglucosamine. Transmission electron microscopy indicated that the hfsDAB mutants fail to synthesize a holdfast at the stalk tip. The predicted hfs gene products have significant sequence similarity to proteins necessary for exopolysaccharide export in gram-negative bacteria. HfsA has sequence similarity to GumC from Xanthomonas campestris, which is involved in exopolysaccharide export in the periplasm. HfsD has sequence similarity to Wza from Escherichia coli, an outer membrane protein involved in secretion of polysaccharide through the outer membrane. HfsB is a novel protein involved in holdfast biogenesis. These data suggest that the hfs genes play an important role in holdfast export.

scholarly journals Dissection of functional domains of the polar localization factor PodJ in Caulobacter crescentus

2006 ◽  
Vol 59 (1) ◽  
pp. 301-316 ◽  
Author(s):  
Melanie L. Lawler ◽  
David E. Larson ◽  
Aaron J. Hinz ◽  
David Klein ◽  
Yves V. Brun
Keyword(s):  
Caulobacter Crescentus ◽  
Functional Domains ◽  
Polar Localization ◽  
Localization Factor

The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease

2016 ◽  
Vol 23 (8) ◽  
pp. 705-713 ◽  
Author(s):  
Daniela Lazzaretti ◽  
Katharina Veith ◽  
Katharina Kramer ◽  
Claire Basquin ◽  
Henning Urlaub ◽  
...  
Keyword(s):  
Rna Binding ◽  
Mrna Localization ◽  
Localization Factor

scholarly journals Role of the GGDEF regulator PleD in polar development of Caulobacter crescentus

2003 ◽  
Vol 47 (6) ◽  
pp. 1695-1708 ◽  
Author(s):  
Phillip Aldridge ◽  
Ralf Paul ◽  
Patrick Goymer ◽  
Paul Rainey ◽  
Urs Jenal
Keyword(s):  
Caulobacter Crescentus ◽  
Polar Development

scholarly journals Kajian Isu Pemekaran di Kota Medan: Tinjauan Berdasarkan Kondisi Ketimpangan Wilayah

2015 ◽  
Vol 3 (3) ◽  
pp. 151
Author(s):  
Cynthia Mutiara Pasaribu
Keyword(s):  
Descriptive Analysis ◽  
Statistical Correlation ◽  
Economic Activities ◽  
Social Demography ◽  
Interview Method ◽  
Polar Development ◽  
Infrastructure Services ◽  
North And South ◽  
Development Institution

<p class="Abstract"><em>Generally, disparities between regions and inter-regions happened because of concentration, agglomeration, external factors, globalization, internal factor, grouping, critical growth or polar development, institution involvement in economic activities. Initially development in Medan concentrated in several sub districts as the center of government and trade activities. Based on long-term development plan of Medan 2006-2025, stated that development policy in Medan not notice of the regional gap. Disparities development between north and south of Medan are the problems for segregation issue at fourth sub-districts in the northern part of Medan. The purpose of this research is to analyze the gap condition in economic, social demography and the infrastructure’s service in Medan related to the emergence of segregation issue in North Medan. The analysis that used in research is descriptive statistic analysis, analysis of statistical correlation, crosstab analysis, and verification of statistic results using interview method so that at the end of the research will be generated economic, social demography and infrastructure services index for every sub-district in the Medan to know the development difference. </em><em>The result of correlation analysis shows that there was a correlation between disparity and segregation issue. Descriptive analysis produce the index for 4 sub districts in north side have the medium category while 17 sub districts in the south have a good category. Crosstab analysis shows that there is a correlation between disparities condition and the emergence of segregation issue. Crosstab analysis also shows that variable education facilities (senior high school), population density, and labor variables show the disparity condition significantly.</em></p>

Vibration localization for a rotating mistuning bladed disk with the Coriolis effect by a state-space decoupling method

2017 ◽  
Vol 233 (3) ◽  
pp. 1011-1020 ◽  
Author(s):  
Xuanen Kan ◽  
Zili Xu
Keyword(s):  
State Space ◽  
Natural Frequency ◽  
Coriolis Force ◽  
High Speed ◽  
Forced Response ◽  
Coriolis Effect ◽  
Decoupling Method ◽  
Resonant Amplitude ◽  
Bladed Disk ◽  
Localization Factor

Slight deviations of blades due to manufacturing tolerances can cause mistuning of bladed disk leading to localized vibration, which can accelerate fatigue. Moreover, the rotating blades are subjected to the Coriolis effect and the influence of the Coriolis force on the natural frequencies of high-speed rotational bladed disks such as those of an aero-engine become more apparent. In this paper, the effect of Coriolis force on the forced response localization of a mistuned bladed disk are investigated, for conditions where the natural frequency located in the first and second modal families of the bladed disk. Mistuning is introduced by varying the Young’s modulus of each blade. Due to the asymmetric Coriolis matrix, it is not possible to directly decouple the system. A state-space decoupling method is developed to decouple the system to effectively calculate the forced response of bladed disk with the consideration of the Coriolis effect. The results show that response localization factor is increased by 13.09% considering the Coriolis force compared to the system without considering the Coriolis effect, in the case of where the first modal family is considered. In addition, the response localization factor with the consideration of the Coriolis force is decreased by 30.85% compared to the system without considering the Coriolis force, when the second modal family is considered. It indicates that the forced response localization with the consideration of the Coriolis effect will be changed obviously with the rotational speed increasing, when the concerning natural frequency is located in the first and second modal families. Furthermore, the effect of Coriolis force causes changes in the resonant frequencies and resonant amplitude, but does not introduce additional resonant peaks for the case of the mistuned bladed disk.

Poradenské inštitúcie a ich význam pre prístup k znalostiam v regióne

2021 ◽  
Author(s):  
Michal Hrivnák ◽  
Peter Moritz ◽  
Mária Fáziková ◽  
Jana Jarábková
Keyword(s):  
Private Sector ◽  
21St Century ◽  
Regional Economy ◽  
Public Support ◽  
The State ◽  
Counseling Services ◽  
Counseling Centers ◽  
Localization Factor ◽  
Sources Of Knowledge ◽  
Comparison Of The Results

In the 21st century, knowledge is becoming the main source of competitiveness for private sector actors, but also the main source of territorial competitiveness of regions. The development of counseling centers and counseling services is becoming a tool of public support for the productivity of actors in the regional economy, and at the same time a significant localization factor of allocation of technologically intensive firms. The aim of the paper is to evaluate the state of development of counseling for business in the conditions of Slovakia, to describe the services that counseling provides and specify the knowledge that disseminates in space, with regard to evaluating the sources of knowledge used by counseling centers for their own activities. Based on a comparison of the results, we segmented these institutions according to the framework of services provided and activities implemented.

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