Identification of genes regulated by dark adaptation and far-red light illumination in roots of Arabidopsis thaliana*

2004 ◽  
Vol 27 (11) ◽  
pp. 1387-1394 ◽  
Author(s):  
K. SATO-NARA ◽  
A. NAGASAKA ◽  
H. YAMASHITA ◽  
J. ISHIDA ◽  
A. ENJU ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Dark Adaptation ◽  
Red Light ◽  
Light Illumination

scholarly journals Artificial complementary chromatic acclimation gene expression system in Escherichia coli

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Dwi Ariyanti ◽  
Kazunori Ikebukuro ◽  
Koji Sode
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Light Exposure ◽  
Expression System ◽  
Red Light ◽  
Green Light ◽  
Light Illumination ◽  
Multiple Gene ◽  
Gene Expression System ◽  
Chromatic Acclimation

Abstract Background The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the PtrcΔLacO promoter. Results One G-box was inserted upstream of the cpcG2 promoter and a reporter gene, the rfp gene (green light-induced gene expression), and the other G-box was inserted between the PtrcΔLacO promoter and a reporter gene, the bfp gene (red light-induced gene expression). The Escherichia coli transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the rfp gene were observed, whereas the expression of the bfp gene was repressed. Under red light illumination, the transcription and translation of the bfp gene were observed, whereas the expression of the rfp gene was repressed. During the red and green light exposure cycles at every 6 h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed. Conclusion An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp. PCC6803, in E. coli. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.

scholarly journals Long‐term adaptation of Arabidopsis thaliana to Far‐red light

2021 ◽  
Author(s):  
Chen Hu ◽  
Wojciech J. Nawrocki ◽  
Roberta Croce
Keyword(s):  
Arabidopsis Thaliana ◽  
Red Light

scholarly journals Light Gradient-Based Screening of Arabidopsis thaliana on a 384-Well Type Plant Array Chip

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 191
Author(s):  
Youn-Hee Park ◽  
Je-Kyun Park
Keyword(s):  
Arabidopsis Thaliana ◽  
White Light ◽  
Solid Medium ◽  
Red Light ◽  
Phytochrome B ◽  
Wild Type ◽  
Light Gradient ◽  
Gradient Based ◽  
Light Effects ◽  
Germination And Growth

Arabidopsis thaliana (Arabidopsis), as a model for plant research, is widely used for various aspects of plant science. To provide a more sophisticated and microscopic environment for the germination and growth of Arabidopsis, we report a 384-well type plant array chip in which each Arabidopsis seed is independently seeded in a solid medium. The plant array chip is made of a poly(methyl methacrylate) (PMMA) acrylic material and is assembled with a home-made light gradient module to investigate the light effects that significantly affect the germination and growth of Arabidopsis. The light gradient module was used to observe the growth pattern of seedlings according to the intensity of the white light and to efficiently screen for the influence of the white light. To investigate the response to red light (600 nm), which stimulates seed germination, the light gradient module was also applied to the germination test. As a result, the germination results showed that the plant array chip can be used to simultaneously screen wild type seeds and phytochrome B mutant seeds on a single array chip according to the eight red light intensities.

scholarly journals Arabidopsis chloroplast J protein DJC75/CRRJ mediates nitrate-promoted seed germination in the dark

2020 ◽  
Vol 125 (7) ◽  
pp. 1091-1099
Author(s):  
Huai-Syuan Ciou ◽  
Yu-Lun Tsai ◽  
Chi-Chou Chiu
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Germination Rate ◽  
Red Light ◽  
Phytochrome B ◽  
Biosynthetic Gene ◽  
Unknown Mechanism ◽  
J Domain ◽  
Domain Protein ◽  
J Protein

Abstract Background and Aims Nitrate can stimulate seed germination of many plant species in the absence of light; however, the molecular mechanism of nitrate-promoted seed germination in the dark remains largely unclear and no component of this pathway has been identified yet. Here, we show that a plastid J-domain protein, DJC75/CRRJ, in arabidopsis (Arabidopsis thaliana) is important for nitrate-promoted seed germination in the dark. Methods The expression of DJC75 during imbibition in the dark was investigated. The seed germination rate of mutants defective in DJC75 was determined in the presence of nitrate when light cues for seed germination were eliminated by the treatment of imbibed seeds with a pulse of far-red light to inactivate phytochrome B (phyB), or by assaying germination in the dark with seeds harbouring the phyB mutation. The germination rates of mutants defective in CRRL, a J-like protein related to DJC75, and in two chloroplast Hsp70s were also measured in the presence of nitrate in darkness. Key Results DJC75 was expressed during seed imbibition in the absence of light. Mutants defective in DJC75 showed seed germination defects in the presence of nitrate when light cues for seed germination were eliminated. Mutants defective in CRRL and in two chloroplast Hsp70s also exhibited similar seed germination defects. Upregulation of gibberellin biosynthetic gene GA3ox1 expression by nitrate in imbibed phyB mutant seeds was diminished when DJC75 was knocked out. Conclusions Our data suggest that plastid J-domain protein DJC75 regulates nitrate-promoted seed germination in the dark by upregulation of expression of the gibberellin biosynthetic gene GA3ox1 through an unknown mechanism and that DJC75 may work in concert with chloroplast Hsp70s to regulate nitrate-promoted seed germination. DJC75 is the first pathway component identified for nitrate-promoted seed germination in the dark.

Response of the photosynthetic apparatus to UV-A and red light in the phytochrome B-deficient Arabidopsis thaliana L. hy3 mutant

2016 ◽  
Vol 54 (3) ◽  
pp. 321-330 ◽  
Author(s):  
V. D. Kreslavski ◽  
F. J. Schmitt ◽  
C. Keuer ◽  
T. Friedrich ◽  
G. N. Shirshikova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Red Light ◽  
Photosynthetic Apparatus ◽  
Phytochrome B

Physiology and plastid fine structure of deetiolating Lemna minor

1976 ◽  
Vol 54 (15) ◽  
pp. 1819-1826 ◽  
Author(s):  
Hugh Frick ◽  
Raymond F. Jones
Keyword(s):  
Higher Plants ◽  
Lemna Minor ◽  
Red Light ◽  
Acid Synthesis ◽  
Light Illumination ◽  
Fresh Weight ◽  
Plastid Development ◽  
Prolamellar Bodies ◽  
Rate Of Increase ◽  
Etiolated Plants

During the 12-h lag period in chlorophyll accumulation after the onset of white-light illumination of Lemna minor etiolated for 35 days, a rapid increase in visible fronds per culture occurred. This new frond production then assumed a log-linear rate of increase, and total protein per unit fresh weight came to parallel the rate of increase in fresh weight per plant. The ribosomal RNA content of 45-day-etiolated plants was deficient in 23S and 16S species compared with green plants. The prolamellar bodies of etioplasts were either tightly or loosely paracrystalline within the same cell; they were without extended perforate lamellae, which developed during far-red-light illumination even while prolamellar bodies persisted. The development of chloroplasts in deetiolating L. minor was typical of other higher plants. The developmental sequence in green Lemna included proplastid to deeply stacked granal chloroplast within several millimetres. Plastid profiles suggestive of division configurations occurred only in primordial cells of green and etiolated plants. The relatively small numbers of plastids in any given stage of differentiation may account for the sensitivity of plastid development to inhibitors of protein and nucleic acid synthesis.

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