Multiplex polymerase chain reaction with DNA pooling: a cost-effective strategy of genotyping rare blood types

Les pneumonies acquises à l’hôpital (PAH) sont fréquentes. À l’ère des techniques diagnostiques de biologie moléculaire (multiplex polymerase chain reaction), les rares données disponibles estiment que les virus respiratoires sont impliqués dans 22 à 32 % des épisodes. Les patients immunodéprimés constituent probablement la population la plus à risque. La présentation clinique et radiologique ne diffère pas entre pneumonies bactériennes, virales et mixtes (virus–bactérie). L’excrétion prolongée de virus respiratoires dans les voies aériennes a été rapportée chez les patients immunodéprimés. Elle pourrait promouvoir la co-infection bactérienne, associée à des durées d’hospitalisation prolongées. L’acquisition intrahospitalière a été démontrée chez tous les virus respiratoires. Elle encourage la mise en œuvre et le respect des mesures d’hygiène et de confinement, dans l’objectif de protéger soignants, visiteurs et patients. De nombreux points restent largement méconnus, relatifs aux interactions entre virus respiratoires et pathogènes non viraux, aux périodes d’incubation, ou encore aux durées d’excrétion virale. L’amélioration des techniques diagnostiques et l’accumulation de données épidémiologiques et cliniques devraient permettre de mieux appréhender le rôle des virus respiratoires dans les PAH. Cette meilleure connaissance aidera à rationaliser l’utilisation des tests de détection et facilitera l’interprétation de leurs résultats. Elle guidera aussi le clinicien dans l’utilisation future des nombreuses molécules antivirales actuellement en développement clinique chez l’homme.

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Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

Infectious Disorders - Drug Targets ◽

10.2174/1871526518666181113091322 ◽

2019 ◽

Vol 19 (3) ◽

pp. 322-326 ◽

Cited By ~ 1

Author(s):

Hassan Valadbeigi ◽

Elham Esmaeeli ◽

Sobhan Ghafourian ◽

Abbas Maleki ◽

Nourkhoda Sadeghifard

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Multiplex Polymerase Chain Reaction ◽

Virulence Genes ◽

Uropathogenic Escherichia Coli ◽

Chain Reaction ◽

Polymerase Chain ◽

Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

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Application of reverse transcriptase nested, hemi-nested And multiplex polymerase chain reaction techniques For the detection of pv 11, pv 3462 and cvs Strains of rabies virus

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2018.9.3.b280-286 ◽

2018 ◽

Vol 9 (3) ◽

Author(s):

THANGARAJ SEKAR ◽

GANESAN CHANDRA MOHAN ◽

BALA SUBRAMANIAN KARTHICK ◽

ANANDA ARONE PREMKUMAR ◽

BHEEMAN SUNDARAN ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcriptase ◽

Rabies Virus ◽

Multiplex Polymerase Chain Reaction ◽

Chain Reaction ◽

Polymerase Chain

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Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis

Microorganisms ◽

10.3390/microorganisms9010067 ◽

2020 ◽

Vol 9 (1) ◽

pp. 67

Author(s):

Seung-Min Yang ◽

Jiwon Baek ◽

Eiseul Kim ◽

Hyeon-Be Kim ◽

Seyoung Ko ◽

...

Keyword(s):

Public Health ◽

Polymerase Chain Reaction ◽

Bacterial Species ◽

Cost Effective ◽

The Other ◽

Gene Marker ◽

Diagnostic Assay ◽

Chain Reaction ◽

Polymerase Chain ◽

Substantial Economic Burden

In recent years, Salmonella Infantis has become a predominant serovariant in clinical and poultry isolates, thereby imposing a substantial economic burden on both public health and the livestock industry. With the aim of coping with the steep increase in serovar Infantis prevalence, a polymerase chain reaction (PCR)-based rapid and accurate diagnostic assay was developed in this study through pangenome profiling of 60 Salmonella serovars. A gene marker, SIN_02055, was identified, which is present in the S. Infantis genome but not in the pangenome of the other serovars. Primers specific to SIN_02055 were used to accurately detect serovar Infantis, and to successfully differentiate Infantis from the other 59 serovars in real-time PCR with a R2 of 0.999 and an efficiency of 95.76%. The developed method was applied to 54 Salmonella strains belonging to eight dominant serovars, and distinguished Infantis from the other seven serovars with an accuracy of 100%. The diagnostic primer set also did not show false positive amplification with 32 strains from eight non-Salmonella bacterial species. This cost-effective and rapid method can be considered an alternative to the classic serotyping using antisera.

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