Association between human leukocyte antigen-G genotype and success of in vitro fertilization and pregnancy outcome

2004 ◽  
Vol 64 (1) ◽  
pp. 66-69 ◽  
Author(s):  
T.V.F. Hviid ◽  
S. Hylenius ◽  
A. Lindhard ◽  
O.B. Christiansen
Keyword(s):  
In Vitro Fertilization ◽  
Human Leukocyte Antigen ◽  
Pregnancy Outcome ◽  
Human Leukocyte ◽  
Vitro Fertilization ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

scholarly journals Human Herpesvirus 6A and 6B inhibit in vitro angiogenesis by induction of Human Leukocyte Antigen G

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Roberta Rizzo ◽  
Maria D’Accolti ◽  
Daria Bortolotti ◽  
Francesca Caccuri ◽  
Arnaldo Caruso ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Herpesvirus ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G ◽  
In Vitro Angiogenesis

scholarly journals Targeting human leukocyte antigen G with chimeric antigen receptors of natural killer cells convert immunosuppression to ablate solid tumors

2021 ◽  
Vol 9 (10) ◽  
pp. e003050
Author(s):  
Chia-Ing Jan ◽  
Shi-Wei Huang ◽  
Peter Canoll ◽  
Jeffrey N Bruce ◽  
Yu-Chuan Lin ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Natural Killer ◽  
Solid Tumors ◽  
Low Dose ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Low Dose Chemotherapy ◽  
Human Leukocyte Antigen G

BackgroundImmunotherapy against solid tumors has long been hampered by the development of immunosuppressive tumor microenvironment, and the lack of a specific tumor-associated antigen that could be targeted in different kinds of solid tumors. Human leukocyte antigen G (HLA-G) is an immune checkpoint protein (ICP) that is neoexpressed in most tumor cells as a way to evade immune attack and has been recently demonstrated as a useful target for chimeric antigen receptor (CAR)-T therapy of leukemia by in vitro studies. Here, we design and test for targeting HLA-G in solid tumors using a CAR strategy.MethodsWe developed a novel CAR strategy using natural killer (NK) cell as effector cells, featuring enhanced cytolytic effect via DAP12-based intracellular signal amplification. A single-chain variable fragment (scFv) against HLA-G is designed as the targeting moiety, and the construct is tested both in vitro and in vivo on four different solid tumor models. We also evaluated the synergy of this anti-HLA-G CAR-NK strategy with low-dose chemotherapy as combination therapy.ResultsHLA-G CAR-transduced NK cells present effective cytolysis of breast, brain, pancreatic, and ovarian cancer cells in vitro, as well as reduced xenograft tumor growth with extended median survival in orthotopic mouse models. In tumor coculture assays, the anti-HLA-G scFv moiety promotes Syk/Zap70 activation of NK cells, suggesting reversal of the HLA-G-mediated immunosuppression and hence restoration of native NK cytolytic functions. Tumor expression of HLA-G can be further induced using low-dose chemotherapy, which when combined with anti-HLA-G CAR-NK results in extensive tumor ablation both in vitro and in vivo. This upregulation of tumor HLA-G involves inhibition of DNMT1 and demethylation of transporter associated with antigen processing 1 promoter.ConclusionsOur novel CAR-NK strategy exploits the dual nature of HLA-G as both a tumor-associated neoantigen and an ICP to counteract tumor spread. Further ablation of tumors can be boosted when combined with administration of chemotherapeutic agents in clinical use. The readiness of this novel strategy envisions a wide applicability in treating solid tumors.

The effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology—a multicenter study

2013 ◽  
Vol 100 (5) ◽  
pp. 1303-1309 ◽  
Author(s):  
Dirk Kotze ◽  
Thinus F. Kruger ◽  
Carl Lombard ◽  
Trishanta Padayachee ◽  
Levent Keskintepe ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Assisted Reproductive Technology ◽  
Pregnancy Outcome ◽  
Multicenter Study ◽  
Biochemical Marker ◽  
Human Leukocyte ◽  
Reproductive Technology ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

Human Leukocyte Antigen G (HLA-G) Gene Expression is Regulated by Progesterone In Vitro

2000 ◽  
Vol 74 (3) ◽  
pp. S9
Author(s):  
S.M Yie ◽  
L.H Li ◽  
J Cross ◽  
C.L Librach
Keyword(s):  
Gene Expression ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

scholarly journals Human Leukocyte Antigen-G Is Frequently Expressed in Glioblastoma and May Be Induced in Vitro by Combined 5-Aza-2′-Deoxycytidine and Interferon-γ Treatments

2013 ◽  
Vol 182 (2) ◽  
pp. 540-552 ◽  
Author(s):  
Isabela J. Wastowski ◽  
Renata T. Simões ◽  
Layale Yaghi ◽  
Eduardo A. Donadi ◽  
João T. Pancoto ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Interferon Γ ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

Expression of human leukocyte antigen-G in systemic lupus erythematosus

2008 ◽  
Vol 69 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Silvia Rosado ◽  
Gema Perez-Chacon ◽  
Susana Mellor-Pita ◽  
Inmaculada Sanchez-Vegazo ◽  
Carmen Bellas-Menendez ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Human Leukocyte Antigen ◽  
Lupus Erythematosus ◽  
Human Leukocyte ◽  
Systemic Lupus ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

Impact of ovarian endometrioma on oocytes and pregnancy outcome in in vitro fertilization

2005 ◽  
Vol 83 (4) ◽  
pp. 908-913 ◽  
Author(s):  
Takahiro Suzuki ◽  
Shun-ichiro Izumi ◽  
Hidehiko Matsubayashi ◽  
Hideo Awaji ◽  
Kikuo Yoshikata ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Outcome ◽  
Vitro Fertilization ◽  
Ovarian Endometrioma
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