The Relationship Between Peroxidase Activity and the Resistance of Tomatoes (Lycopersicum esculentum) to Verticillium dahliae

1985 ◽  
Vol 112 (3) ◽  
pp. 193-197 ◽  
Author(s):  
R. Reuveni ◽  
J. F. Ferreira
Keyword(s):  
Verticillium Dahliae ◽  
Peroxidase Activity ◽  
Lycopersicum Esculentum ◽  
The Relationship

scholarly journals Peroxidase activity in human cutaneous mast cells: an ultrastructural demonstration.

1984 ◽  
Vol 32 (6) ◽  
pp. 573-578 ◽  
Author(s):  
L M Escribano ◽  
L C Gabriel ◽  
T Sainz ◽  
A Rocamora ◽  
J M Arrazola ◽  
...  
Keyword(s):  
Mast Cell ◽  
Enzyme Activity ◽  
Mast Cells ◽  
Peroxidase Activity ◽  
Ultrastructural Level ◽  
Prostaglandin Synthesis ◽  
Cell Disease ◽  
Mast Cell Disease ◽  
Cytochemical Technique ◽  
The Relationship

An intense and reproducible peroxidase staining in the cutaneous mast cells of two patients with systemic mast cell disease and urticaria pigmentosa is demonstrated at the ultrastructural level. This enzyme activity was demonstrated by use of a cytochemical technique employing 3,3'- diaminobenzicine (DAB) as an oxidizable substrate, after fixation by a tannic acid-aldehyde mixture. Enzyme activity was localized in the perinuclear cisterna and strands of endoplasmic reticulum. Granules appeared unreactive. This peroxidase activity appears sensitive to fixation by aldehydes; it is inhibited by 3-amino-1,2,4-triazole (AMT) and by lack of H2O2 or DAB in the incubation medium. These characteristics are fundamentally different from the peroxidase activity of basophils, and the demonstration of this enzyme is therefore not a further argument for a common ontogenetic origin of both cells. On the other hand, the cytochemical characteristics of this enzyme are very similar to those of platelet peroxidase (P-PO), which has been connected to the synthesis by platelets of prostaglandins. Since the mast cell is known to generate prostaglandins, the relationship between the enzyme described and prostaglandin synthesis by mast cells is discussed.

scholarly journals Influence of Inoculum Density of Verticillium dahliae on Root Discoloration of Horseradish

Plant Disease ◽  
2000 ◽  
Vol 84 (3) ◽  
pp. 309-315 ◽  
Author(s):  
A. Khan ◽  
N. Atibalentja ◽  
D. M. Eastburn
Keyword(s):  
Verticillium Dahliae ◽  
Commercial Production ◽  
Inoculum Density ◽  
Forecast System ◽  
Factors Affecting ◽  
Initial Inoculum ◽  
Disease Forecast ◽  
Low Levels ◽  
Negative Exponential ◽  
The Relationship

The relationship between inoculum density (number of microsclerotia per gram of air-dried soil) of Verticillium dahliae at the time of planting and the severity and incidence of root discoloration of horseradish at harvest was investigated in a 2-year study conducted in the greenhouse, microplots, and commercial production fields. The objective of the study was to develop a disease-forecast system that would assist growers in assessing the risk of the disease before planting horseradish in a particular field. Significant correlations were observed between inoculum density and severity and incidence of root discoloration in the greenhouse and microplots, although the form of the relationship varied with trials from linear to quadratic and negative exponential. No correlation was found between inoculum density of V. dahliae and severity and incidence of root discoloration in commercial production fields. In some fields with low inoculum densities, high ratings of severity and incidence of root discoloration were observed even with the partially resistant cultivar 769A. Conversely, in other fields with high inoculum densities, low ratings of severity and incidence of discolored roots were observed even with the susceptible cultivar 647A. These results suggest that a disease-forecast system based solely on inoculum densities of V. dahliae would be unreliable under field conditions when the other factors affecting the inoculum density-disease relationships cannot be controlled. Knowing the amount of initial inoculum may, however, save growers from planting horseradish in highly infested fields, but it would not guarantee a disease-free crop in fields with low levels of infestation.

scholarly journals Inoculum Density-Disease Development Relationship in Verticillium Wilt of Artichoke Caused by Verticillium dahliae

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1131-1136 ◽  
Author(s):  
M. Berbegal ◽  
A. Ortega ◽  
J. García-Jiménez ◽  
J. Armengol
Keyword(s):  
Population Dynamics ◽  
Verticillium Dahliae ◽  
Symptom Severity ◽  
Disease Incidence ◽  
Growing Season ◽  
Inoculum Density ◽  
Disease Development ◽  
Growing Seasons ◽  
Negative Exponential ◽  
The Relationship

The relationship between inoculum density of Verticillium dahliae in soil and disease development was studied in 10 commercial artichoke fields. Inoculum density of V. dahliae varied between 2.2 and 34.2 microsclerotia (ms) g–1 of soil near planting. Artichoke plants were monitored for disease at the beginning and the end of each growing season. There was a significant correlation, which was best described by negative exponential models, between inoculum density and disease incidence, symptom severity, and recovery of the pathogen from the plants. Inoculum densities ranging from 5 to 9 ms g–1 of soil were associated with a mean percentage of infected plants of about 50%. Additionally, three fields were monitored in two consecutive growing seasons to evaluate the population dynamics of V. dahliae microsclerotia in soil and disease development. Numbers of microsclerotia per gram of soil decreased significantly by the end of the first growing season but slightly increased at the end of the second growing season. In these fields, symptom severity was greatest during the second growing season when high percentages of infected plants also were recorded.

scholarly journals Studies on Wheat Peroxidase

1969 ◽  
Vol 22 (1) ◽  
pp. 261 ◽  
Author(s):  
RW Parish
Keyword(s):  
Peroxidase Activity ◽  
Tobacco Leaf ◽  
The Relationship ◽  
Leaf Disks

Recently, workers in this laboratory have studied the increased synthesis of peroxidase in senescing tobacco leaf disks (Parish 1968a, 1968b) and the relationship between lignification and peroxidase activity in wheat internodes (Parish and Miller 1968). The present paper reports four simple experiments designed to further clarify the interrelationships between lignification, maturation, and peroxidase activity.

Colonization of tomatoes by Verticillium dahliae: determinative phase II

1996 ◽  
Vol 74 (8) ◽  
pp. 1279-1288 ◽  
Author(s):  
Julie Gold ◽  
Barbara Lee ◽  
J. Robb
Keyword(s):  
Verticillium Dahliae ◽  
Wilt Disease ◽  
Symptom Expression ◽  
Race 1 ◽  
Race 2 ◽  
The Relationship ◽  
Ve Gene ◽  
Defensive Mechanism ◽  
Colonizing Ability

To investigate the relationship between stem colonizing ability of Verticillium and wilt disease development, the colonization capacities of Verticillium dahliae, race 1 or race 2, were compared in near-isolines of the cultivar Craigella, with (Ve+) and without (Ve−) Ve-gene resistance, and in the cultivar IRAT-L3, which also lacks the Ve-gene. Only the Ve+/V.d.1 interaction was resistant to disease. Lateral and vertical invasiveness of the pathogen were assessed cytologically and fungal biomass was monitored by PCR-based diagnostics. The pathogen was most aggressive in the Ve−/V.d.1 interaction; colonization capacity was reduced in the other five combinations, but most severely, and equivalently, in Ve+/V.d.1, IRAT/V.d.1, and IRAT/V.d.2. Further study of the development and role of the vascular coating response in IRAT-L3 indicated a pattern of expression similar to that observed in other susceptible plants, and it was concluded that the reduced colonization of this cultivar by V. dahliae resulted from another unknown defensive mechanism. Stem colonizing ability that is compatible with resistance in one cultivar and race combination may promote symptom expression and even death in another. Keywords: tomato, Verticillium dahliae, vascular coating, resistance.

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