Characterisation of Pga1, a putative Candida albicans cell wall protein necessary for proper adhesion and biofilm formation

Mycoses ◽  
2010 ◽  
Vol 54 (6) ◽  
pp. 491-500 ◽  
Author(s):  
Rami Hashash ◽  
Samer Younes ◽  
Wael Bahnan ◽  
Joseph El Koussa ◽  
Katia Maalouf ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Cell Wall Protein

scholarly journals Candida albicans Sun41p, a Putative Glycosidase, Is Involved in Morphogenesis, Cell Wall Biogenesis, and Biofilm Formation

2007 ◽  
Vol 6 (11) ◽  
pp. 2056-2065 ◽  
Author(s):  
Ekkehard Hiller ◽  
Sonja Heine ◽  
Herwig Brunner ◽  
Steffen Rupp
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Cell Monolayer ◽  
The Sun ◽  
Calcofluor White ◽  
Cellular Processes ◽  
Cell Wall Biogenesis ◽  
Reduced Adherence ◽  
Higher Sensitivity

ABSTRACT The SUN gene family has been defined in Saccharomyces cerevisiae and comprises a fungus-specific family of proteins which show high similarity in their C-terminal domains. Genes of this family are involved in different cellular processes, like DNA replication, aging, mitochondrial biogenesis, and cytokinesis. In Candida albicans the SUN family comprises two genes, SUN41 and SIM1. We demonstrate that C. albicans mutants lacking SUN41 show similar defects as found for S. cerevisiae, including defects in cytokinesis. In addition, the SUN41 mutant showed a higher sensitivity towards the cell wall-disturbing agent Congo red, whereas no difference was observed in the presence of calcofluor white. Compared to the wild type, SUN41 deletion strains exhibited a defect in biofilm formation, a reduced adherence on a Caco-2 cell monolayer, and were unable to form hyphae on solid medium under the conditions tested. Interestingly, Sun41p was found to be secreted in the medium of cells growing as blastospores as well as those forming hyphae. Our results support a function of SUN41p as a glycosidase involved in cytokinesis, cell wall biogenesis, adhesion to host tissue, and biofilm formation, indicating an important role in the host-pathogen interaction.

scholarly journals Critical role for CaFEN1 and CaFEN12 of Candida albicans in cell wall integrity and biofilm formation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Md. Alfatah ◽  
Vinay K. Bari ◽  
Anubhav S. Nahar ◽  
Swati Bijlani ◽  
K. Ganesan
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Critical Role ◽  
Cell Wall Integrity

The Candida albicans cell wall protein Rhd3/Pga29 is abundant in the yeast form and contributes to virulence

Yeast ◽  
2010 ◽  
Vol 27 (8) ◽  
pp. 611-624 ◽  
Author(s):  
Albert D. de Boer ◽  
Piet W. J. de Groot ◽  
Günther Weindl ◽  
Martin Schaller ◽  
Dietmar Riedel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein ◽  
Yeast Form

scholarly journals Hwp1 and Related Adhesins Contribute to both Mating and Biofilm Formation in Candida albicans

2009 ◽  
Vol 8 (12) ◽  
pp. 1909-1913 ◽  
Author(s):  
Iuliana V. Ene ◽  
Richard J. Bennett
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Sexual Differentiation ◽  
Biofilm Formation ◽  
Filamentous Growth ◽  
Cell Wall Proteins ◽  
Related Cell

ABSTRACT Candida albicans Hwp1, Hwp2, and Rbt1 are related cell wall proteins expressed during the programs of sexual differentiation and filamentous growth. In this study, we compare strains lacking either single factors or a combination of these genes, and we demonstrate distinct but overlapping roles in mating and biofilm formation.

scholarly journals Serologic Response to Cell Wall Mannoproteins and Proteins of Candida albicans

1998 ◽  
Vol 11 (1) ◽  
pp. 121-141 ◽  
Author(s):  
José P. Martínez ◽  
M. Luisa Gil ◽  
José L. López-Ribot ◽  
W. LaJean Chaffin
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Antibody Response ◽  
Humoral Response ◽  
Binding Activity ◽  
Cell Wall Protein ◽  
Wall Structure ◽  
Cell Mediated Immunity ◽  
Host Immune System

SUMMARY The cell wall of Candida albicans not only is the structure in which many biological functions essential for the fungal cells reside but also is a significant source of candidal antigens. The major cell wall components that elicit a response from the host immune system are proteins and glycoproteins, the latter being predominantly mannoproteins. Both the carbohydrate and protein moieties are able to trigger immune responses. Although cell-mediated immunity is often considered to be the most important line of defense against candidiasis, cell wall protein and glycoprotein components also elicit a potent humoral response from the host that may include some protective antibodies. Proteins and glycoproteins exposed at the most external layers of the wall structure are involved in several types of interactions of fungal cells with the exocellular environment. Thus, coating of fungal cells with host antibodies has the potential to influence profoundly the host-parasite interaction by affecting antibody-mediated functions such as opsonin-enhanced phagocytosis and blocking the binding activity of fungal adhesins for host ligands. In this review, the various members of the protein and glycoprotein fraction of the C. albicans cell wall that elicit an antibody response in vivo are examined. Although a number of proteins have been shown to stimulate an antibody response, for some of these species the response is not universal. On the other hand, some of the studies demonstrate that certain cell wall antigens and anti-cell wall antibodies may be the basis for developing specific and sensitive serologic tests for the diagnosis of candidasis, particularly the disseminated form. In addition, recent studies have focused on the potential for antibodies to cell wall protein determinants to protect the host against infection. Hence, a better understanding of the humoral response to cell wall antigens of C. albicans may provide the basis for the development of (i) effective procedures for the serodiagnosis of disseminated candidiasis and (ii) novel prophylactic (vaccination) and therapeutic strategies for the management of this type of infection.

scholarly journals The Adhesin Hwp1 and the First Daughter Cell Localize to the a/a Portion of the Conjugation Bridge duringCandida albicansMating

2003 ◽  
Vol 14 (12) ◽  
pp. 4920-4930 ◽  
Author(s):  
Karla J. Daniels ◽  
Shawn R. Lockhart ◽  
Janet F. Staab ◽  
Paula Sundstrom ◽  
David R. Soll
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Mating Type ◽  
Daughter Cell ◽  
Cell Wall Protein ◽  
Parent Cell ◽  
A Cells ◽  
Α Pheromone

The cell wall protein Hwp1 was originally demonstrated to be expressed exclusively in hyphae of Candida albicans and cross-linked to human epithelium by mammalian transglutaminase. Hwp1 is expressed on the walls of hyphae formed by a/α, a/a, and α/α cells. Hence, it is expressed on hyphae independently of mating type. However, Hwp1 is selectively expressed on the wall of conjugation tubes formed by a/a cells, but not α/α cells, in the mating process. This was demonstrated in all possible crosses between four unrelated natural a/a strains and four unrelated α/α strains. In zygotes, Hwp1 is restricted to that portion of the wall of the conjugation bridge contributed by the a/a parent cell. Hwp1 staining further revealed that the first daughter bud that emerges from the conjugation bridge does so from the a/a-contributed portion. Hwp1 expression and localization during the mating process is, therefore, mating type specific, opaque phase specific, and α-pheromone induced. These results indicate that the mating type-specific contributions to the conjugation bridge during the mating process in C. albicans are qualitatively and functionally distinct and that the a/a portion of the bridge, which selectively contains Hwp1, bears the first daughter cell in the mating process.

scholarly journals Characterization of Hwp2, a Candida albicans putative GPI-anchored cell wall protein necessary for invasive growth

2010 ◽  
Vol 165 (3) ◽  
pp. 250-258 ◽  
Author(s):  
Peter Hayek ◽  
Leila Dib ◽  
Pascal Yazbeck ◽  
Berna Beyrouthy ◽  
Roy A. Khalaf
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein ◽  
Invasive Growth
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