Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and coefficients of parentage

2004 ◽  
Vol 123 (4) ◽  
pp. 366-369 ◽  
Author(s):  
S. Maric ◽  
S. Bolaric ◽  
J. Martincic ◽  
I. Pejic ◽  
V. Kozumplik
Euphytica ◽  
2007 ◽  
Vol 163 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Fatemeh Amini ◽  
Ghodratollah Saeidi ◽  
Ahmad Arzani

2017 ◽  
Vol 18 (4) ◽  
pp. 1632-1637
Author(s):  
VIET THE HO ◽  
QUOC NGUYEN NGO

Ho VT, Ngo QNg. 2017. Short Communication: Using RAPD technique to evaluate genetic diversity of longan (Dimocarpus longan) population in Vietnam. Biodiversitas 18: xxxx. Longan (Dimocarpus longan L.) is an important fruit plant in Vietnam where several cultivars and landraces are being cultivated. Presently, the identification of longan cultivars and landraces is mainly based on personal experience relying on morphological traits leading the difficulty of genetic conservation and longan breeding. In this study, genetic richness and relativeness of 36 longan accessions collected from different provinces in Vietnam were evaluated by using 30 Random Amplified Polymorphic DNA (RAPD) primers. Our results reveal that there is the large variation of genetic background of studied longan accessions with separate groups in the obtained dendrogram. We also identified the combination of different RAPD markers could help to identify 15 longan genotypes. The results from this project could provide valuable information which is necessary for classifying, identifying plant origins, breeding and conserving programs of longan in Vietnam.


2005 ◽  
Vol 56 (12) ◽  
pp. 1355 ◽  
Author(s):  
Anna Mantzavinou ◽  
Penelope J. Bebeli ◽  
Pantouses J. Kaltsikes

Using the random amplified polymorphic DNA (RAPD) method, the genetic diversity of 19 Greek landraces and 9 cultivars of durum wheat [Triticum turgidum L. var. durum (Desf.)] was studied. Two commercial bread wheat (Triticum aestivum L.) cultivars and one genotype of Triticum monococcum L. were also included in the study. Eighty-seven arbitrary primers (10-mer) were evaluated in a preliminary experiment and 15 of them were selected for the main experiments based on the quality and reliability of their amplification and the polymorphism they revealed. A total of 150 DNA bands were obtained, 125 (83.3%) of which were polymorphic. On average, 10 DNA bands were amplified per primer, 8.3 of which were polymorphic. The genetic similarity between all pairs of genotypes was evaluated using the Jaccard’s or Nei and Li’s coefficients; the values of the former ranged from 0.153 to 0.973 while those of the latter were slightly higher (0.265–0.986). Cluster analysis was conducted by the UPGMA and the Njoin methods. Both methods broadly placed 26 durum genotypes into 1 branch while the other branch consisted of 2 subgroups: 1 included the 2 bread wheat cultivars; the other 1 consisted of 2 durum landraces, ‘Kontopouli’ and ‘Mavrotheri-Chios’, which showed an intruiging behaviour sharing bands with the bread wheat cultivars. The T. monococcum cultivar stood apart from all other genotypes.


2011 ◽  
Vol 41 (No. 2) ◽  
pp. 73-78 ◽  
Author(s):  
L. Milella ◽  
J. Salava ◽  
G. Martelli ◽  
I. Greco ◽  
E.F. Cusimamani ◽  
...  

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon.  


Author(s):  
Sachin Kumar ◽  
Ajay Kumar ◽  
Manoj Kumar Sharma ◽  
Manoj Kumar Sharma

In the present study, morphological and genetic diversity is revealed among the four species of Ocimum. Among the morphological characters plant height, the number of leaves, leaf area, leaf width, leaf length, stem circumference and mitotic index studied and contributed a greater proportion of variations. RAPD markers revealed a high degree of polymorphism (88.89%) among the species of Ocimum. Dendrogram study revealed that the species O. gratissimum and O. basilicum are very closely related whereas O. kilimandscharicum and O. sanctum are distantly related in the phylogenetic history.


2013 ◽  
Vol 158 ◽  
pp. 1-7 ◽  
Author(s):  
Monia Bendhifi ◽  
Ghada Baraket ◽  
Lazhar Zourgui ◽  
Sami Souid ◽  
Amel Salhi-Hannachi

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