Immunohistochemical Identification of Lymphocyte Subsets and Macrophages in Normal Human Urothelium Using Monoclonal Antibodies

1986 ◽  
Vol 58 (2-4) ◽  
pp. 436-442 ◽  
Author(s):  
M. I. M. EI-DEMIRY ◽  
T. B. HARGREAVE ◽  
A. BUSUTTIL ◽  
K. JAMES ◽  
G. D. CHISHOLM
Keyword(s):  
Monoclonal Antibodies ◽  
Lymphocyte Subsets ◽  
Normal Human ◽  
Immunohistochemical Identification ◽  
Normal Human Urothelium

scholarly journals Determination of platelet antigens and glycoproteins in the human fetus

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 488-492 ◽  
Author(s):  
Y Gruel ◽  
B Boizard ◽  
F Daffos ◽  
F Forestier ◽  
J Caen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Fetus ◽  
Antenatal Diagnosis ◽  
Polyclonal Antibodies ◽  
Umbilical Vein ◽  
Membrane Surface ◽  
Platelet Suspension ◽  
Direct Puncture ◽  
Normal Human

Abstract The autosomal recessive transmission of Glanzmann's thrombasthenia (GT) and Bernard-Soulier syndrome (BSS), together with requests of families who already had children with these diseases, prompted us to investigate the feasibility of their antenatal diagnosis. The preliminary step leading to the early detection of GT or BSS was to characterize, in the normal human fetus, the platelet antigens and glycoproteins (GPs) and to define their normal amounts on the membrane surface. Blood samples from 32 fetuses between 18 to 26 weeks of gestation were collected by direct puncture of the umbilical vein using an ultrasound-guided needle. Polyclonal antibodies from human origin directed against PLA1, Leka antigens, and the GPIIb IIIa complex (IgGL), or murine monoclonal antibodies specific for GPIb (AN51, 6D1), GPIIIa (AP-3), or GPIIb IIIa (AP-2) were studied using platelet suspension immunofluorescence tests. The binding of each antibody was quantified using a cytofluorograph (Ortho 50H). PLA1 and Leka antigens were expressed in normal amounts on fetal platelets as early as 16 weeks of intrauterine life. The GPIIb IIIa complex quantified by polyclonal or monoclonal antibodies was in the same range in fetuses (IgGL = 427 +/- 23 AUF, AP-2 = 459.5 +/- 8.5; AP-3 = 536 +/- 14) and in adults (IgGL = 420 +/- 30; AP-2 = 498 +/- 11; AP-3 = 515 +/- 13). The platelet binding of antibodies that recognized GPIb was higher in fetuses (AN51 = 491.5 +/- 14; 6D1 = 479 +/- 15) than in adults (AN51 = 426.5 +/- 9; 6D1 = 449 +/- 8.7). These results suggest that immunological techniques can be applied as early as 18 weeks of gestation for the antenatal diagnosis of GT and BSS.

Immunohistochemical identification of the pacemaker cajal cells in the normal human vagina

2005 ◽  
Vol 272 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Ahmed Shafik ◽  
Olfat El-Sibai ◽  
Ismail Shafik ◽  
Ali A. Shafik
Keyword(s):  
Cajal Cells ◽  
Human Vagina ◽  
Normal Human ◽  
Immunohistochemical Identification

scholarly journals Distribution of lymphocytes of the α E β 7 phenotype and E-cadherin in normal human urothelium and bladder carcinomas

2001 ◽  
Vol 126 (3) ◽  
pp. 397-402 ◽  
Author(s):  
J. Cresswell ◽  
H. Robertson ◽  
D. E. Neal ◽  
T. R. L. Griffiths ◽  
J. A. Kirby
Keyword(s):  
Normal Human ◽  
Bladder Carcinomas ◽  
Normal Human Urothelium ◽  
E Cadherin

Monoclonal Antibodies That Define T-Lymphocyte Subsets in the Rat

1980 ◽  
pp. 251-273 ◽  
Author(s):  
Donald W. Mason ◽  
Roger J. Brideau ◽  
W. Robert McMaster ◽  
Michael Webb ◽  
Robert A. H. White ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets

scholarly journals Human gamma interferon enhances release from phytohemagglutinin- stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1339-1347
Author(s):  
W Piacibello ◽  
L Lu ◽  
D Williams ◽  
M Aglietta ◽  
BY Rubin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
Progenitor Cells ◽  
Lymphocyte Subsets ◽  
Human Peripheral Blood ◽  
Gamma Interferon ◽  
Cell Sorter ◽  
Enhancing Effect ◽  
Macrophage Colony

Human gamma interferon (HuIFN gamma) was evaluated for its effects on the release from human peripheral blood T lymphocytes (greater than 98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granulocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFN gamma. In the presence of PHA, pure natural HuIFN gamma at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFN gamma-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFN gamma were neutralized with a monoclonal anti-natural HuIFN gamma, and recombinant HuIFN gamma mimicked the enhancing effects of the natural HuIFN gamma. This enhancing effect was noted only when HuIFN gamma was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (greater than 98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFN gamma was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFN gamma is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFN gamma and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.

Clonal analysis of expression of epithelial antigens in cultures of normal human breast

1986 ◽  
Vol 80 (1) ◽  
pp. 91-101
Author(s):  
P.A. Edwards ◽  
I.M. Brooks ◽  
H.J. Bunnage ◽  
A.V. Foster ◽  
M.L. Ellison ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Phase Contrast ◽  
Single Cells ◽  
Full Range ◽  
Human Breast ◽  
Intact Tissue ◽  
Normal Human Breast ◽  
Normal Human ◽  
Luminal Epithelial Cells

Cells from normal human breast epithelium were cloned in monolayer culture and the clones were stained with monoclonal antibodies. Tissue was from reduction mammoplasty operations. Cloning efficiencies were 5–30%. Two types of clone were identified: 10 to 30% were of relatively spread cells whose boundaries were often difficult to see by phase-contrast microscopy but where they were visible they appeared as dark lines. The edges of the clones usually appeared to be under tension. These clones were stained by two monoclonal antibodies, LICR-LON-M8 and M24, that stain luminal epithelial cells in the intact tissue, but not myoepithelial or stromal cells. Within a clone the cells showed a full range of antigenic phenotypes. This was confirmed for clones grown from single cells that had been isolated manually. The second type of clone was more compact with little evidence of tension at the edges, and cell boundaries were clearly visible and bright under phase contrast. These clones were not stained by antibodies M8 or M24. Both types of clone stained with a third monoclonal antibody that is specific for luminal epithelial cells in the intact tissue, LICR-LON-M18, but the distribution of staining was different in the different types of clone. The simplest interpretation of the two types of clone is that luminal epithelial cells give rise to the spread type of clone while the myoepithelial cells give rise to the more abundant and vigorous compact clones. Alternatively, the compact clones may be from luminal epithelial cells that have lost differentiated characteristics.

Sign in / Sign up

Export Citation Format

Share Document