In vivoLocalisation of Human Urinary Bladder Carcinoma Xenograft in Nude Mice Using Radiolabeled Monoclonal Antibody

1987 ◽  
Vol 59 (6) ◽  
pp. 540-546 ◽  
Author(s):  
D. S. YÜ ◽  
M. Y. YEH ◽  
W. L. CHEN ◽  
S. Y. CHANG ◽  
C. P. MA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Urinary Bladder ◽  
Nude Mice ◽  
Bladder Carcinoma ◽  
Urinary Bladder Carcinoma ◽  
Human Urinary Bladder ◽  
Carcinoma Xenograft

scholarly journals Expression of GATA3 and Cytokeratin 14 in Urinary Bladder Carcinoma (Histopathological and Immunohistochemical Study)

2021 ◽  
Vol 9 (A) ◽  
pp. 858-864
Author(s):  
Nora Elzohery ◽  
Nourelhoda Sayed Ismael ◽  
Rasha Ahmed Khairy ◽  
Somia A. M. Soliman
Keyword(s):  
Monoclonal Antibody ◽  
Squamous Cell Carcinoma ◽  
Urinary Bladder ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Bladder Carcinoma ◽  
Urinary Bladder Carcinoma ◽  
Immunohistochemical Expression ◽  
Immunohistochemical Marker ◽  
Cytokeratin 14

BACKGROUND: Urothelial carcinoma (UC) with squamous differentiation (SD) is the most common histologic variant of bladder carcinoma and its presence is associated with poor prognosis which may need early radical cystectomy to avoid progression and recurrence. It is difficult to detect few foci of SD, especially nonkeratinizing or early switch from urothelial to squamous epithelium on only morphological basis. Combination of GATA3 and Cytokeratin 14 (CK14) could be helpful in differentiating pure UC, UC with SD and pure squamous cell carcinoma (SCC). AIM: Assessment of GATA3 and CK14 expression in urinary bladder carcinoma and correlation with clinical and histopathological variables, for both diagnostic and prognostic purposes. MATERIALS AND METHODS: Sixty cases of archived paraffin blocks of urinary bladder carcinoma were tested for GATA3 and CK14 expression by immunohistochemistry using a rabbit monoclonal antibody against human CK 14 and mouse monoclonal antibody against GATA3, respectively. RESULTS: There is a significant correlation between GATA3 immunohistochemical expression and histological tumor subtypes of bladder carcinoma (p < 0.001), i.e. the GATA3 is a useful marker for urothelial origin especially in papillary UC. There is a significant correlation between GATA3 immunohistochemical expression and UC grade (p < 0.001). CK14 showed positive cytoplasmic staining in 9/14 (64.3%) cases of UC with SD and (13/13) (100%) cases of pure SCC and negative in 33/33(100%) cases of UC other than UC with SD. CK14 had sensitivity (64.3%) and specificity (100%) for areas of SD. CONCLUSION: GATA3 is a specific immunohistochemical marker for urothelial origin. CK14 is a highly specific and sensitive immunohistochemical marker of squamous cell carcinoma.

scholarly journals THE EXPRESSION OF THE E-CADHERIN IN HUMAN URINARY BLADDER CARCINOMA

1995 ◽  
Vol 86 (7) ◽  
pp. 1208-1215 ◽  
Author(s):  
Jiro Miyazaki ◽  
Masato Fujisawa ◽  
Soichi Arakawa ◽  
Sadao Kamidono
Keyword(s):  
Urinary Bladder ◽  
Bladder Carcinoma ◽  
Urinary Bladder Carcinoma ◽  
Human Urinary Bladder ◽  
E Cadherin

scholarly journals Dominative role of MMP-14 over MMP-15 in human urinary bladder carcinoma on the basis of its enhanced specific activity

Medicine ◽  
2020 ◽  
Vol 99 (7) ◽  
pp. e19224
Author(s):  
Jacek Kudelski ◽  
Grzegorz Młynarczyk ◽  
Barbara Darewicz ◽  
Marta Bruczko-Goralewska ◽  
Lech Romanowicz
Keyword(s):  
Urinary Bladder ◽  
Bladder Carcinoma ◽  
Specific Activity ◽  
Urinary Bladder Carcinoma ◽  
Human Urinary Bladder

scholarly journals Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma

2021 ◽  
Vol 10 (16) ◽  
pp. 3683
Author(s):  
Jacek Kudelski ◽  
Grzegorz Młynarczyk ◽  
Monika Gudowska-Sawczuk ◽  
Barbara Mroczko ◽  
Barbara Darewicz ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Matrix Metalloproteinases ◽  
Urinary Bladder ◽  
Matrix Metalloproteinase ◽  
Bladder Carcinoma ◽  
Urinary Bladder Cancer ◽  
Urinary Bladder Carcinoma ◽  
Low Grade ◽  
High Grade ◽  
Human Urinary Bladder

Human urinary bladder cancer is a huge worldwide oncological problem causing many deaths every year. The degradation of extracellular matrix (ECM) induced by molecules such as matrix metalloproteinases (MMPs) is one of the main factors influencing the process of metastasis origination. The MMP expression is tied to tumor aggressiveness, stage, and patient prognosis. The cleavage of constituent proteins is initiated and prolonged by matrix metalloproteinases, such as MMP-3 and MMP-10. The aim of this study was to evaluate the expression and activity of both MMPs in human urinary bladder cancer occurring at various stages of the disease. Tissue samples from patients with urinary bladder cancer were analyzed. Samples were collected from patients with a low- and high-grade cancer. Control tissue was collected from the site opposite to the tumor. DNA content, MMPs content, and activity of MMP-3 and MMP-10 were measured using ELISA and Western blot techniques. MMP-3 and MMP-10 occur in high molecular complexes in human urinary bladder in healthy and cancerous tissues. Particularly, in high-grade tumors, the content of MMP-10 prevails over MMP-3. The actual and specific activities vary in both grades of urinary bladder cancer; however, the highest activity for MMP-3 and MMP-10 was found in low-grade tissues. In conclusion, MMP-10 had a higher content, but a lower activity in all investigated tissues compared to MMP-3. Generally, obtained results demonstrated a contrary participation of MMP-3 and MMP-10 in ECM remodeling what may be crucial in the pathogenesis of human urinary bladder carcinoma.

Sign in / Sign up

Export Citation Format

Share Document