scholarly journals THE EFFECT OF COPPER ON NITROGEN FIXATION IN SUBTERRANEAN CLOVER (TRIFOLIUM SUBTERRANEUM)

1980 ◽  
Vol 85 (1) ◽  
pp. 63-72 ◽  
Author(s):  
K. SNOWBALL ◽  
A. D. ROBSON ◽  
J. F. LONERAGAN
1954 ◽  
Vol 5 (2) ◽  
pp. 247 ◽  
Author(s):  
JR Harris

A study was made of a number of effective and ineffective strains of Rhizobium trifolii in association with subterranean clover (Trifolium subterraneum L.), variety Bacchus Marsh. Where these were inoculated singly into sterilized soils in pot experiments conducted in the greenhouse, typical effective, ineffective, or intermediate plant reactions were obtained. Where more than one strain was inoculated, competition between strains took place and was reflected in nodule pattern and plant reaction. Some strains consistently failed to produce appreciable nodules in the presence of other rhizobia, some showed modification to varying degrees, and some were unaffected. Such behaviour was found to be closely linked with the ability of the strain to proliferate in the rhizosphere of the host plant, and placement of inoculum was shown to influence plant reaction. The concept of "incursion" as a property of a rhizobial strain is suggested. An incursive strain is one able to migrate from the initial site of inoculation and establish an adequate population in the root zone of the susceptible host despite the presence of active elements of the indigenous microflora and microfauna, including antagonistic and predatory forms. The property of incursion is independent of considerations of efficiency of nitrogen fixation or virulence for the host plant. Strains of rhizobia which are poorly incursive may fail to infect the host upon which they have been inoculated if subject to competition from indigenous rhizobia already established in the soil. The necessity of assessing properties of virulence and incursion as well as efficiency of nitrogen fixation in selecting rhizobia for purposes of commercial inoculation is stressed, and the methods of making such tests are discussed.


1951 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
RC Rossiter

The results of pot-culture experiments and field trials designed to examine the effects of copper, zinc, and potassium on the growth of Dwalganup subterranean clover on a number of Western Australian soils are presented and discussed. Highly significant yield increases from application of one or more nutrients were observed on all soils examined. The effects of applied copper were greater in the second year than in the seeding year under deficiency conditions in the field. Significant interaction effects were observed only with copper and potassium. Maximum yields in two of the field trials were low even with application of all three nutrients. Reasons for this are suggested. Data on leaf area changes indicated that, in contrast to the increasing severity of potassium deficiency with age of the plant, both copper and zinc deficiency tended to diminish after the commencement of the flowering stage. The importance of such time trends in the interpretation of interaction effects is emphasized. The economic significance of the potassium problem is stressed and a number of aspects requiring investigation are outlined.


1959 ◽  
Vol 12 (1) ◽  
pp. 26 ◽  
Author(s):  
JF Loneragan

It has been shown that calcium has a specific effect upon nitrogen fixation by subterranean clover (Trifolium subterraneum. L.).


1953 ◽  
Vol 4 (2) ◽  
pp. 151 ◽  
Author(s):  
KD McLachlan

The results of a field experiment on two acid podzolized soils in the Heytesbury area, Victoria, are presented. Nutrient elements were tested on a phalaris (Phalaris tuberosa L.) - subterranean clover (Trifolium subterraneum L.) pasture on an area cleared from heath and on an adjoining area cleared from timber with an understorey of heath. Responses of clover to lime and copper, and interactions between these treatments were obtained. There was no response to potash, zinc, manganese, molybdenum, iron, magnesium, or boron on either soil. Lime increased the yield of clover at both sites, the greater effect occurring on the area cleared from timber. The effect of lime was not due to the release of soil molybdenum. Lime corrected defective nodulation, increased the survival of the plants, and to some extent also increased the yield of individual plants. The relative significance of these effects of lime in determining the final yield of clover per acre is discussed. The effect of copper was very different on the two soils. On the area cleared from heath, copper deficiency was severe. Here, copper increased the yield in the absence as well as in the presence of lime. The effect was considerably greater in the presence of lime, and this positive interaction was highly significant. Copper increased the number of plants per unit area and the yield of individual clover plants. On the area cleared from timber, copper decreased the yield in the absence of lime. Where lime had been applied, the effect of copper on the yield, though not significant, was positive. The evidence presented indicates that copper is also required on this soil. A copper deficiency symptom of subterranean clover is described. It is suggested that the copper status of the soils may influence the distribution of trees in the area.


2000 ◽  
Vol 51 (3) ◽  
pp. 377 ◽  
Author(s):  
G. M. Lodge

Seedlings of 3 perennial grasses, Danthonia linkii Kunthcv. Bunderra, D. richardsonii Cashmore cv. Taranna(wallaby grasses), and Phalaris aquatica L. cv. Sirosa,were each grown in replacement series mixtures with seedlings ofTrifolium repens L. (white clover),Trifolium subterraneum L. var. brachycalycinum (Katzn.et Morley) Zorahy & Heller cv. Clare (subterraneanclover), and Lolium rigidum L. (annual ryegrass). Plantswere sown 5 cm apart in boxes (45 by 29 by 20 cm) at a density of 307plants/m2. Maximum likelihood estimates were usedto derive parameters of a non-linear competition model using the dry matterweights of perennial grasses and competitors at 3 harvests, approximately 168,216, and 271 days after sowing. Intra-plant competition was examined inmonocultures of each species, grown at plant spacings of 2, 5, and 8 cm apartwith plants harvested at the above times.Competition occurred in all perennial grass–competitor mixtures, exceptin those of each perennial grass with white clover and thephalaris–subterranean clover mixture (Harvest 1) and those withD. richardsonii and phalaris grown with white clover(Harvest 2). For D. richardsonii (Harvests 1 and 2) andD. linkii (Harvest 1 only) grown with white clover andthe phalaris–subterranean clover (Harvest 1), the two species in themixture were not competing. In the phalaris–white clover mixture, eachspecies was equally competitive (Harvests 1 and 2). These differences incompetition and aggressiveness reflected differences in individual plantweights in monocultures where there was an effect (P < 0.05) of species ondry matter weight per box, but no significant effect of plant spacing.These data indicated that for successful establishment,D. richardsonii and D. linkiishould not be sown in swards with either subterranean clover or white clover,or where populations of annual ryegrass seedlings are likely to be high.Phalaris was more compatible with both white clover and subterranean clover,but aggressively competed with by annual ryegrass.


2004 ◽  
Vol 94 (5) ◽  
pp. 433-439 ◽  
Author(s):  
S.N. Johnson ◽  
P.J. Gregory ◽  
P.J. Murray ◽  
X Zhang ◽  
I.M. Young

AbstractThis study investigated the ability of neonatal larvae of the root-feeding weevil, Sitona lepidus Gyllenhal, to locate white clover Trifolium repens L. (Fabaceae) roots growing in soil and to distinguish them from the roots of other species of clover and a co-occurring grass species. Choice experiments used a combination of invasive techniques and the novel technique of high resolution X-ray microtomography to non-invasively track larval movement in the soil towards plant roots. Burrowing distances towards roots of different plant species were also examined. Newly hatched S. lepidus recognized T. repens roots and moved preferentially towards them when given a choice of roots of subterranean clover, Trifolium subterraneum L. (Fabaceae), strawberry clover Trifolium fragiferum L. (Fabaceae), or perennial ryegrass Lolium perenneL. (Poaceae). Larvae recognized T. repens roots, whether released in groups of five or singly, when released 25 mm (meso-scale recognition) or 60 mm (macro-scale recognition) away from plant roots. There was no statistically significant difference in movement rates of larvae.


1956 ◽  
Vol 7 (2) ◽  
pp. 98 ◽  
Author(s):  
JN Black

Changes in the pre-emergence distribution of dry matter in subterranean clover (Trifolium subterraneum L.) variety Bacchus Marsh were followed at 21°C, using three sizes of seed and three depths of sowing, ½, 1¼, and 2 in. Decreasing seed size and increasing depth of sowing both reduce the weight of the cotyledons a t emergence. Seed of the three sizes were sown a t three depths in pot culture a t staggered intervals so that emergence was simultaneous. Dry weight in the early vegetative stage was proportional to seed size, and total leaf area and leaf numbers showed similar trends. Plants of each seed size grew at the same relative rate. No effect of depth of sowing could be detected, and this was shown to be due to the cotyledon area a t emergence being constant for any given seed size, regardless of varying depth of sowing and hence of cotyledon weight. It was concluded that seed size in a plant having epigeal germination and without endosperm is of importance: firstly, in limiting the maximum hypocotyl elongation and hence depth of sowing, and secondly, in determining cotyledon area. Cotyledon area in turn influences seedling growth, which is not affected by cotyledon weight. Once emergence has taken place, cotyledonary reserves are of no further significance in the growth of the plants.


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