scholarly journals Determination of G and P type diversity of group A rotaviruses in faecal samples of diarrhoeic calves in Kashmir, India

2010 ◽  
Vol 51 (5) ◽  
pp. 595-599 ◽  
Author(s):  
S.A. Beg ◽  
S.A. Wani ◽  
I. Hussain ◽  
M.A. Bhat
Keyword(s):  
Faecal Samples ◽  
Group A Rotaviruses ◽  
Group A ◽  
P Type

scholarly journals Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Heliyon ◽  
2020 ◽  
Vol 6 (8) ◽  
pp. e04521
Author(s):  
Abhay Raorane ◽  
Zunjar Dubal ◽  
Sandeep Ghatak ◽  
Michael Mawlong ◽  
B. Susngi ◽  
...  
Keyword(s):  
Human Group ◽  
Group A Rotaviruses ◽  
Group A

G and P types of bovine group A rotavirus in northern India

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
Tripti Singh ◽  
Rashmi Singh ◽  
Ajay Pratap Singh ◽  
Y. P.S. Malik ◽  
Minakshi Prasad
Keyword(s):  
Interspecies Transmission ◽  
Buffalo Calves ◽  
Potential Threat ◽  
Specific Primers ◽  
Northern India ◽  
Faecal Samples ◽  
Group A Rotaviruses ◽  
Group A ◽  
Vp6 Gene ◽  
Faecal Suspension

Animal rotaviruses (RVs) are considered as a potential threat to humans due to possibility of interspecies transmission and exchange of genomic materials. Among several RVs, the group A rotaviruses (RVA) are the major cause of diarrhoea in cattle and buffalo calves worldwide. The present study was carried out to understand epidemiology and types of RVA circulating in Mathura region of northern India. One hundred faecal samples were collected from diarrhoeic cattle (n=94) and buffalo calves (n=6) from organized dairy farms. Viral RNA was extracted from faecal suspension and was transcribed to cDNA using RT-PCR. The cDNA was amplified for VP6, VP7 and VP4 genes and multiplex nested PCR was done for G and P genotyping. Twelve samples were detected positive for RVA by antigen detection ELISA and eleven samples produced expected amplicon for group A specific VP6 gene. On genotyping with G3, G6, G8 and G10 specific primers for VP7 gene and P [1] and P [11] specific primers for VP4 gene, G6 genotype predominated over other genotypes. Dual genotypes were also observed. In P typing only one isolate was found to be positive for P [1] and rest all samples remained untypable. The result of present study indicates G6 as a major G genotype and change in frequencies of distribution of G types in this part of the country.

Prevalence and genotypic (G and P) determination of porcine group A rotaviruses from different regions of India

2012 ◽  
Vol 45 (2) ◽  
pp. 609-615 ◽  
Author(s):  
Zunjar Baburao Dubal ◽  
Kiran N. Bhilegaonkar ◽  
Sukhadeo B. Barbuddhe ◽  
Rahul P. Kolhe ◽  
Simranpreet Kaur ◽  
...  
Keyword(s):  
Group A Rotaviruses ◽  
Group A

scholarly journals Unusual rotavirus genotypes in humans and animals with acute diarrhoea in Northeast India

2016 ◽  
Vol 144 (13) ◽  
pp. 2780-2789 ◽  
Author(s):  
P. CHAKRABORTY ◽  
M. J. BHATTACHARJEE ◽  
I. SHARMA ◽  
P. PANDEY ◽  
N. N. BARMAN
Keyword(s):  
Vaccine Development ◽  
Acute Diarrhoea ◽  
Northeast India ◽  
Post Vaccination ◽  
Infantile Diarrhoea ◽  
Faecal Samples ◽  
Close Proximity ◽  
Global Spread ◽  
Group A Rotaviruses ◽  
Group A

SUMMARYRotavirus (RV) infection causes acute infantile diarrhoea in humans and animals and remains a major concern for vaccine development. The close proximity of humans to animals may foster cross-species infection resulting in the emergence of novel/unusual strains by genetic reassortment. In this study, we characterized 500 diarrhoeal samples for group A rotaviruses (RVA) from children (n= 290), piglets (n= 95) and calves (n= 115) in Northeast India during 2012–2013. The data showed that 142/500 (28·4%) faecal samples were positive for RVA with the highest level of infection detected in piglets (57/142, 40·1%) followed by children (51/142, 35·9%) and calves (34/142, 23·9%). Sequence-based G- and P-typing showed G1P[8] (25%) and G1P[7] (35%) were the prevailing genotypes in both humans and animals. Single cases of unusual genotypes, i.e. G9P[8], G5P[8] in humans and G1P[13], G1P[23] and G3P[7] in animals were also identified. Cluster analyses of the sequences showed regional strains were genetically closer to their homologous strains. However, human G5P[8] and porcine G1P[8] strains showed homology to heterologous hosts of their prototype strains. The subsequent global spread of unusual RV strains may result in their establishment over time, presenting challenges to future vaccine evaluation programmes. More studies on emerging genotypes are required to elucidate how RVA strains evolve post-vaccination. This study supports the need for continuous surveillance of RVA infections after detecting from diverse hosts in a common setting.

scholarly journals Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

2009 ◽  
Vol 54 (No. 1) ◽  
pp. 12-18 ◽  
Author(s):  
R. Smitalova ◽  
L. Rodak ◽  
B. Smid ◽  
I. Psikal
Keyword(s):  
Nucleotide Sequence ◽  
Nucleotide Sequence Identity ◽  
Sequence Identity ◽  
Faecal Samples ◽  
Two Samples ◽  
Pcr Products ◽  
Group A Rotaviruses ◽  
Group A ◽  
Cowden Strain ◽  
Group B

Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain.

Subgroup Determination of Group A Rotaviruses Recovered from Piglets in Nigeria

1995 ◽  
Vol 8 (3) ◽  
pp. 151-157 ◽  
Author(s):  
D.J.I. ATII ◽  
C.K. OJEH
Keyword(s):  
Group A Rotaviruses ◽  
Group A

Rotavirus electropherotypes in Malaysian children

1992 ◽  
Vol 38 (9) ◽  
pp. 996-999 ◽  
Author(s):  
K. L. Yap ◽  
Y. H. Wong ◽  
C. M. Khor ◽  
Y. E. Ooi
Keyword(s):  
Molecular Epidemiology ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Older Children ◽  
Migration Patterns ◽  
Faecal Samples ◽  
Group A Rotaviruses ◽  
Group A ◽  
Short Pattern ◽  
Predominant Strain

A 12-month study was carried out on the molecular epidemiology of rotavirus in urban and suburban Malaysian children. Analysis of faecal samples from 973 hospitalized diarrhoeic children by polyacrylamide gel electrophoresis detected 268 rotaviruses (28%). All isolates were group A rotaviruses, which produced 22 electropherotypes: 16 (91.5%) with long RNA migration patterns and 6 (8.5%) with short patterns. One of the long-pattern electropherotypes was the predominant strain (71.1% of the total electropherotypes) isolated during this study. Although 3 other strains were detected sporadically over the study period, 16 others were present only during the first 7 months and 2 others were confined to the last 5 months. Long- and short-pattern electropherotypes were found to co-circulate extensively. There was a significant association of short-pattern electropherotypes with infection in older children. In addition, the prevalence of vomiting and mean duration of diarrhoea were significantly associated with different electropherotypes. Key words: rotavirus electropherotypes.

Determination of arsenic atom distribution arsenic doped silicon using HOLZ-line analysis

1996 ◽  
Vol 54 ◽  
pp. 976-977
Author(s):  
Y. Kikuchi ◽  
N. Hashikawa ◽  
F. Uesugi ◽  
E. Wakai ◽  
K. Watanabe ◽  
...  
Keyword(s):  
Inelastic Scattering ◽  
Zone Axis ◽  
Experimental Result ◽  
Background Intensity ◽  
Crystal Thickness ◽  
Arsenic Atom ◽  
Doped Silicon ◽  
P Type ◽  
Line Analysis

In order to measure the concentration of arsenic atoms in nanometer regions of arsenic doped silicon, the HOLZ analysis is carried out underthe exact [011] zone axis observation. In previous papers, it is revealed that the position of two bright lines in the outer SOLZ structures on the[011] zone axis is little influenced by the crystal thickness and the background intensity caused by inelastic scattering electrons, but is sensitive to the concentration of As atoms substitutbnal for Siatomic site.As the result, it becomes possible to determine the concentration of electrically activated As atoms in silicon within an observed area by means of the simple fitting between experimental result and dynamical simulatioan. In the present work, in order to investigate the distribution of electrically activated As in silicon, the outer HOLZ analysis is applied using a nanometer sized probe of TEM equipped with a FEG.Czodiralsld-gown<100>orientated p-type Si wafers with a resistivity of 10 Ώ cm are used for the experiments.TheAs+ implantation is performed at a dose of 5.0X1015cm-2at 25keV.

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