Rotavirus electropherotypes in Malaysian children

1992 ◽  
Vol 38 (9) ◽  
pp. 996-999 ◽  
Author(s):  
K. L. Yap ◽  
Y. H. Wong ◽  
C. M. Khor ◽  
Y. E. Ooi
Keyword(s):  
Molecular Epidemiology ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Older Children ◽  
Migration Patterns ◽  
Faecal Samples ◽  
Group A Rotaviruses ◽  
Group A ◽  
Short Pattern ◽  
Predominant Strain

A 12-month study was carried out on the molecular epidemiology of rotavirus in urban and suburban Malaysian children. Analysis of faecal samples from 973 hospitalized diarrhoeic children by polyacrylamide gel electrophoresis detected 268 rotaviruses (28%). All isolates were group A rotaviruses, which produced 22 electropherotypes: 16 (91.5%) with long RNA migration patterns and 6 (8.5%) with short patterns. One of the long-pattern electropherotypes was the predominant strain (71.1% of the total electropherotypes) isolated during this study. Although 3 other strains were detected sporadically over the study period, 16 others were present only during the first 7 months and 2 others were confined to the last 5 months. Long- and short-pattern electropherotypes were found to co-circulate extensively. There was a significant association of short-pattern electropherotypes with infection in older children. In addition, the prevalence of vomiting and mean duration of diarrhoea were significantly associated with different electropherotypes. Key words: rotavirus electropherotypes.

scholarly journals Electropherotypes and G-Types of Group A Rotaviruses Detected in Children with Diarrhea in Lagos, Nigeria

ISRN Virology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Christianah Idowu Ayolabi ◽  
David Ajiboye Ojo ◽  
George Enyimah Armah
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Migration Pattern ◽  
Genomic Diversity ◽  
Rt Pcr ◽  
Group A Rotaviruses ◽  
Group A ◽  
Stool Samples ◽  
Health Care Centers ◽  
Virus Isolates

Approximately over 500,000 children die annually due to severe dehydrating diarrhea caused by rotaviruses. This work investigated rotavirus infection among children less than 5 years with diarrhea in Lagos and determined the circulating electropherotypes and genotypes of the virus isolates. Three hundred and two (n=302) stool samples from children below 60 months were collected from different hospitals and health care centers in Lagos and subjected to enzyme immunoassay (EIA) to determine the presence of Group A rotavirus, RT-PCR to determine the G-types, and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes. The results show that 60.3% of the samples showed distinct rotavirus RNA migration pattern, having long electropherotypes (55.3%) of seven variations dominating over the short electropherotypes (44.5%). Six different G-types were detected (G1, G2, G3, G4, G9, and G12). Serotypes G1 and G12 showed long electropherotypic pattern while G2, G3, and G9 exhibited either short or long electropherotype. All G4 detected show short electropherotypic pattern. In conclusion, information on the genomic diversity and RNA electropherotypes of rotaviruses detected in children with diarrhea in Lagos is reported in this study.

scholarly journals Group B rotaviruses similar to strain CAL-1, have been circulating in Western India since 1993

2004 ◽  
Vol 132 (4) ◽  
pp. 745-749 ◽  
Author(s):  
S. D. KELKAR ◽  
J. K. ZADE
Keyword(s):  
Severe Disease ◽  
Polyacrylamide Gel Electrophoresis ◽  
Epidemiological Studies ◽  
Western India ◽  
Human Group ◽  
Older Children ◽  
Pcr Products ◽  
Group A Rotaviruses ◽  
Group A ◽  
Group B

Generally, group A rotaviruses are the most common cause of paediatric diarrhoea. However, group B rotavirus, adult diarrhoea rotavirus (ADRV), was found to be involved in epidemics of severe gastroenteritis in several areas of China during 1982–1983 and had resulted in more than one million cases among adults as well as older children. Human group B rotavirus has been rarely reported outside China, but has been detected first from five adults with diarrhoea in Kolkata, India during 1997–1998 (strain CAL-1). During epidemiological studies at the National Institute of Virology (NIV) on hospitalized diarrhoea patients at Pune, India, faecal specimens from patients of >5 years age, which were negative for group A rotavirus by ELISA were tested by polyacrylamide gel electrophoresis (PAGE). We detected rotavirus RNA migration patterns similar to that of group B rotavirus in three faecal specimens from adults, two from the specimens collected in 1993 and one in 1998 from sporadic diarrhoea cases. RT–PCR was carried out using primers derived from gene 8 which codes for the NS2 protein, followed by nested PCR, which confirmed the presence of group B rotavirus in all three specimens. The sequences of the PCR products of NIV specimens were compared with that of CAL-1, ADRV and IDIR (infectious diarrhoea of infant rat) belonging to group B rotaviruses. The sequence analysis of the PCR products showed the highest identity with CAL-1, which was reported from Kolkata, India during 1997–1998. The finding suggests that human group B rotaviruses have been circulating in Pune, India, since 1993. This emerging virus may lead to more severe disease among adults in India. There is a need for surveillance of group B rotavirus infections, especially in adult diarrhoea cases and seroepidemiological studies on group B rotavirus are required among humans and animals of Western Maharashtra, India.

Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

2018 ◽  
Author(s):  
Rinsha Balan ◽  
M. Mini ◽  
P. M. Priya ◽  
Siju Joseph ◽  
Surya Sankar
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Virus Detection ◽  
Migration Pattern ◽  
Polyacrylamide Gel ◽  
Rota Virus ◽  
Poultry Birds ◽  
Faecal Samples ◽  
Group A ◽  
Different Parts

The present work was conducted to determine the prevalence of rotavirus infection among poultry birds having diarrhoea. A total of 143 faecal samples were collected from different parts of Kerala and screened for the presence of rotavirus using RNA polyacrylamide gel electrophoresis (RNA-PAGE). Out of 143 samples, 5 (3.49%) were found to be positive in RNA PAGE with a migration pattern 4:2:3:2 of a mammalian-like electropherogroup A rotavirus. The study records the first evidence of rota virus detection from avian species in Kerala.

Molecular epidemiologic study on avian rotavirus prevailing in Bangladesh

1970 ◽  
pp. 43-48
Author(s):  
MR Karim ◽  
FI Rume ◽  
MM Alam ◽  
MU Ahmed
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Epidemiologic Study ◽  
Summer Season ◽  
Migration Speed ◽  
Migration Patterns ◽  
Rotavirus Infection ◽  
Faecal Samples ◽  
Group D ◽  
Molecular Epidemiologic Study

The present study on molecular epidemiologic study on avian rotavirus prevailing in Bangladesh was attempted to know the present status of avian rotavirus in Bangladesh. A total of 210 faecal samples of chickens were collected from August 2006 to March 2007 and examined by polyacrylamide gel electrophoresis and silver staining (PAGE-ss) technique for the presence of rotavirus dsRNA. The prevalence of avian rotavirus infection was 13.81% in chickens. The prevalence of avian rotavirus infection in Chittagong, Mymensingh, Gazipur and Barisal were 2.86%, 21.25%, 5% and 22.5%, respectively. The highest prevalence was recorded in Barisal (22.5%) and the lowest in Chittagong (2.86%). The highest prevalence (40%) was observed in 12 day-old birds and the lowest (5.71%) in 22 days old birds. The highest percentage (18%) of avian rotavirus was detected in the summer season. The migration patterns of all detected positive strains were similar in gel electrophoresis and their migration speed was same as previously designated avian rotavirus group D. Key words: Epidemiology, PAGE, rotavirus, chickens DOI = 10.3329/bjvm.v5i1.1308 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 43-48

scholarly journals Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces

1997 ◽  
Vol 68 (3) ◽  
Author(s):  
M. De Beer ◽  
I. Peenze ◽  
V.M. Da Costa Mendes ◽  
A.D. Steele
Keyword(s):  
Electron Microscopy ◽  
Gold Standard ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Latex Agglutination ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Rotavirus ◽  
Group A Rotaviruses ◽  
Group A

The performance characteristics of 2 enzyme immunoassays (ELISAs) and 4 latex agglutination assays (LXs) were evaluated for the detection of bovine rotavirus in faecal specimens of young calves with diarrhoea. A total of 26 specimens from calves less than 5 months of age were examined with different commercial assays and compared with electron microscopy (EM) as the gold standard and with polyacrylamide gel electrophoresis (PAGE) for the detection of atypical, non-group A rotaviruses. In the 2nd study, EIA (Dako) and LX (Murex), the assays of choice, were used to analyse 97 further faecal specimens from calves with diarrhoea. The ELISAs proved to be the most sensitive compared with the other tests used. The EM and PAGE are 100 % specific although slightly less sensitive than the commercial assays. The results show that all the commercial assays can accurately detect rotavirus in the stools of calves with gastroenteritis, although the suitability and choice of assay will depend upon the requirements of individual laboratories.

scholarly journals Characterization of rotavirus electropherotypes excreted by symptomatic and asymptomatic infants

1991 ◽  
Vol 106 (1) ◽  
pp. 189-198 ◽  
Author(s):  
J. Fernández ◽  
A. M. Sandino ◽  
J. Pizarro ◽  
L. F. Avendaño ◽  
J. M. Pizarro ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Mixed Infections ◽  
Migration Patterns ◽  
Virulent Strains ◽  
Human Rotavirus ◽  
Stool Samples

SUMMARYHuman rotavirus isolates from 1100 stool samples were analyzed by polyacrylamide gel electrophoresis, and 48 different migration patterns were detected. Heterogeneity in the migration of segment 10 was observed in both long and short electropherotypes in which three long and two short patterns were identified. In spite of these variations all short and long electropherotypes were subgrouped by enzyme immunoassay as subgroups I and II respectively. Mixed infections were detected in 17% of cases and the subgrouping correlated with the corresponding electropherotypes. The same electropherotypes were present in severe, mild and asymptomatic cases and no electropherotype was particularly associated with greater virulence. Furthermore, the electropherotypes isolated from nosocomial asymptomatic cases were the same as those detected from those admitted with severe diarrheoa. It seems unlikely that electropherotyping can be used to identify more virulent strains of rotavirus.

scholarly journals Immunochemical evidence for an additional H-2 region closely linked to H-2D.

1977 ◽  
Vol 145 (2) ◽  
pp. 438-442 ◽  
Author(s):  
T H Hansen ◽  
S E Cullen ◽  
D H Sachs
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Spleen Cell ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Molecular Size ◽  
Migration Patterns ◽  
Different Types ◽  
D Region ◽  
Chemical Evidence

Anti-H-2 reagents have been tested on solubilized spleen cell preparations in combinations expected to be specific for D region products. Two different types of molecules were detected. One showed the expected reactivity with both antisera to private and antisera to public specificities. However, an additional molecule was detected which reacted only with antisera to public specificities. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis migration patterns indicated that both products have a similar molecular size of approximately 45,000 daltons. The data therefore present chemical evidence for the existence of a third H-2-associated gene product of 45,000 mol wt in addition to the classical H-2K and H-2D antigens.

G and P types of bovine group A rotavirus in northern India

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
Tripti Singh ◽  
Rashmi Singh ◽  
Ajay Pratap Singh ◽  
Y. P.S. Malik ◽  
Minakshi Prasad
Keyword(s):  
Interspecies Transmission ◽  
Buffalo Calves ◽  
Potential Threat ◽  
Specific Primers ◽  
Northern India ◽  
Faecal Samples ◽  
Group A Rotaviruses ◽  
Group A ◽  
Vp6 Gene ◽  
Faecal Suspension

Animal rotaviruses (RVs) are considered as a potential threat to humans due to possibility of interspecies transmission and exchange of genomic materials. Among several RVs, the group A rotaviruses (RVA) are the major cause of diarrhoea in cattle and buffalo calves worldwide. The present study was carried out to understand epidemiology and types of RVA circulating in Mathura region of northern India. One hundred faecal samples were collected from diarrhoeic cattle (n=94) and buffalo calves (n=6) from organized dairy farms. Viral RNA was extracted from faecal suspension and was transcribed to cDNA using RT-PCR. The cDNA was amplified for VP6, VP7 and VP4 genes and multiplex nested PCR was done for G and P genotyping. Twelve samples were detected positive for RVA by antigen detection ELISA and eleven samples produced expected amplicon for group A specific VP6 gene. On genotyping with G3, G6, G8 and G10 specific primers for VP7 gene and P [1] and P [11] specific primers for VP4 gene, G6 genotype predominated over other genotypes. Dual genotypes were also observed. In P typing only one isolate was found to be positive for P [1] and rest all samples remained untypable. The result of present study indicates G6 as a major G genotype and change in frequencies of distribution of G types in this part of the country.

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