scholarly journals Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon,Pyrococcus furiosus: A key role for NADPH

1994 ◽  
Vol 122 (3) ◽  
pp. 245-250 ◽  
Author(s):  
Kesen Ma ◽  
Zhi Hao ◽  
Michael W.W. Adams
Keyword(s):  
Hydrogen Production ◽  
Pyrococcus Furiosus ◽  
Hyperthermophilic Archaeon

scholarly journals Impact of Substrate Glycoside Linkage and Elemental Sulfur on Bioenergetics of and Hydrogen Production by the Hyperthermophilic Archaeon Pyrococcus furiosus

2007 ◽  
Vol 73 (21) ◽  
pp. 6842-6853 ◽  
Author(s):  
Chung-Jung Chou ◽  
Keith R. Shockley ◽  
Shannon B. Conners ◽  
Derrick L. Lewis ◽  
Donald A. Comfort ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Protein Production ◽  
Pyrococcus Furiosus ◽  
Fold Increase ◽  
Gas Production ◽  
Cell Protein ◽  
Response Analysis ◽  
Independent Manner ◽  
Hyperthermophilic Archaeon ◽  
Glycoside Linkage

ABSTRACT Glycoside linkage (cellobiose versus maltose) dramatically influenced bioenergetics to different extents and by different mechanisms in the hyperthermophilic archaeon Pyrococcus furiosus when it was grown in continuous culture at a dilution rate of 0.45 h−1 at 90�C. In the absence of S0, cellobiose-grown cells generated twice as much protein and had 50%-higher specific H2 generation rates than maltose-grown cultures. Addition of S0 to maltose-grown cultures boosted cell protein production fourfold and shifted gas production completely from H2 to H2S. In contrast, the presence of S0 in cellobiose-grown cells caused only a 1.3-fold increase in protein production and an incomplete shift from H2 to H2S production, with 2.5 times more H2 than H2S formed. Transcriptional response analysis revealed that many genes and operons known to be involved in α- or β-glucan uptake and processing were up-regulated in an S0-independent manner. Most differentially transcribed open reading frames (ORFs) responding to S0 in cellobiose-grown cells also responded to S0 in maltose-grown cells; these ORFs included ORFs encoding a membrane-bound oxidoreductase complex (MBX) and two hypothetical proteins (PF2025 and PF2026). However, additional genes (242 genes; 108 genes were up-regulated and 134 genes were down-regulated) were differentially transcribed when S0 was present in the medium of maltose-grown cells, indicating that there were different cellular responses to the two sugars. These results indicate that carbohydrate characteristics (e.g., glycoside linkage) have a major impact on S0 metabolism and hydrogen production in P. furiosus. Furthermore, such issues need to be considered in designing and implementing metabolic strategies for production of biofuel by fermentative anaerobes.

Balancing electron transfer rate and driving force for efficient photocatalytic hydrogen production in CdSe/CdS nanorod–[NiFe] hydrogenase assemblies

2017 ◽  
Vol 10 (10) ◽  
pp. 2245-2255 ◽  
Author(s):  
Bryant Chica ◽  
Chang-Hao Wu ◽  
Yuhgene Liu ◽  
Michael W. W. Adams ◽  
Tianquan Lian ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Hydrogen Production ◽  
Driving Force ◽  
Transfer Rate ◽  
Pyrococcus Furiosus ◽  
Electron Transfer Rate ◽  
Photocatalytic Hydrogen Production ◽  
Photocatalytic System

We describe a hybrid photocatalytic system for hydrogen production consisting of nanocrystalline CdSe/CdS dot-in-rod (DIR) structures coupled to [NiFe] soluble hydrogenase I (SHI) fromPyrococcus furiosus.

scholarly journals A Novel DNA Polymerase Family Found inArchaea

1998 ◽  
Vol 180 (8) ◽  
pp. 2232-2236 ◽  
Author(s):  
Yoshizumi Ishino ◽  
Kayoko Komori ◽  
Isaac K. O. Cann ◽  
Yosuke Koga
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Pyrococcus Furiosus ◽  
Dna Polymerases ◽  
Open Reading Frames ◽  
Gene Products ◽  
Polymerase Gene ◽  
Hyperthermophilic Archaeon ◽  
Dna Polymerase Ii ◽  
Reading Frames

ABSTRACT One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeonPyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499–512, 1997). The genes in M. jannaschiiencoding the proteins that are homologous to the DNA polymerase II ofP. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia colihad both DNA polymerizing and 3′→5′ exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

Effects of Constituents in Bio/Photocatalytic Hydrogen Production System Using Experimental Design Tool

2006 ◽  
Vol 510-511 ◽  
pp. 134-137
Author(s):  
Hyun Ku Joo ◽  
Sang Bong Lee ◽  
Yong Gun Shul ◽  
Jin Wook Ha
Keyword(s):  
Experimental Design ◽  
Hydrogen Production ◽  
Production System ◽  
Pyrococcus Furiosus ◽  
Design Tool ◽  
Fractional Factorial ◽  
Critical Conditions ◽  
Electron Mediator ◽  
Buffer Solution ◽  
Photocatalytic Hydrogen Production

This study investigated the effects of constituents in bio/photocatalytic hydrogen production system using an experimental design tool, a fractional factorial design. The factors considered included species of buffer solution, the amount of hydrogenase (Pyrococcus furiosus, Pfu), electron donor, reaction temperature and electron mediator. The experiments were designed to determine critical conditions that were required to achieve the highest level of H2 production.

scholarly journals A Novel Single-Strand Specific 3′–5′ Exonuclease Found in the Hyperthermophilic Archaeon, Pyrococcus furiosus

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58497 ◽  
Author(s):  
Kazuo Tori ◽  
Sonoko Ishino ◽  
Shinichi Kiyonari ◽  
Saki Tahara ◽  
Yoshizumi Ishino
Keyword(s):  
Pyrococcus Furiosus ◽  
Single Strand ◽  
Hyperthermophilic Archaeon
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